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Q7N8X2

- SYI_PHOLL

UniProt

Q7N8X2 - SYI_PHOLL

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Photorhabdus luminescens subsp. laumondii (strain TT01)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (15 Dec 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
    Binding sitei605 – 6051ATPUniRule annotation
    Metal bindingi900 – 9001ZincUniRule annotation
    Metal bindingi903 – 9031ZincUniRule annotation
    Metal bindingi920 – 9201ZincUniRule annotation
    Metal bindingi923 – 9231ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:plu0591
    OrganismiPhotorhabdus luminescens subsp. laumondii (strain TT01)
    Taxonomic identifieri243265 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePhotorhabdus
    ProteomesiUP000002514: Chromosome

    Organism-specific databases

    GenoListiplu0591.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 937937Isoleucine--tRNA ligasePRO_0000098438Add
    BLAST

    Proteomic databases

    PRIDEiQ7N8X2.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243265.plu0591.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7N8X2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi602 – 6065"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7N8X2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKNTLNL PETGFPMRGD LAKREPNMLK RWYKDELYQV IRKAKAGKKT    50
    FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKGMAGYDSP YIPGWDCHGL 100
    PIELKVEQII GKPGEKFSAA EFRAECRKYA KEQIEGQKKD FIRLGILGDW 150
    ERPYLTMDFK TEADIIRALS RIIANGHLLK GAKPVHWCTD CRSSLAEAEV 200
    EYYDKTSPSI DVRFNAVDAV AVCEKFGVQA PEQPVSLVIW TTTPWTLPAN 250
    RAIALHAEFN YQLVQIEGEC LILAADLVES VMQRAGITSW TVLGHCAGSA 300
    LELLRFKHPF MGFDSPVVLG DHVTLDAGTG AVHTAPGHGP DDFVLGQKYG 350
    LEVANPVGPN GCYLPGTYPS LDGMFVFKAN DVVLNILSEN NALLHLEKLQ 400
    HSYPCCWRHK TPIIFRATPQ WFVSMDQNGL RKQSLQEIKG VQWIPGWGQA 450
    RIEAMVENRP DWCISRQRTW GTPMSLFVHK ETEELHPRTI ELMEEVAKRV 500
    EVDGIQAWWD LEPAELLGDD AANYVKIFDT LDVWFDSGST HASVVDARPE 550
    FQGNAADIYL EGSDQHRGWF MSSLMISTAI KGKAPYRQVL THGFTVDGQG 600
    RKMSKSIGNT ISPQDVMDKL GADILRLWVA STDYTGEIAV SDEILKRSAD 650
    AYRRIRNTAR FLLANLNGFD PEQHSVKPEE MAVLDRWAVG CAQAAQADIA 700
    KCYDKYDFHT VVQRMMQFCS VEMGSFYLDI IKDRQYTAKS DSLARRSCQT 750
    ALYHIAEALV RWMAPILSFT ADEVWNELPG KRAQYVFTEE WYGGLFGLAA 800
    GELMNDAFWA DLLAVRGEVN KVLEQARADK HIRSSLEAAV TLYADNELAD 850
    KLNSLGDELR FVLLTSQVVV ADYEQAGEDA QQSEIGSLKI AFRKADGEKC 900
    PRCWHYAKDV GLVAEHAELC GRCVTNVAGN GEERKFA 937
    Length:937
    Mass (Da):104,977
    Last modified:December 15, 2003 - v1
    Checksum:i54EE6A4747B7DC1A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571860 Genomic DNA. Translation: CAE12886.1.
    RefSeqiNP_927939.1. NC_005126.1.

    Genome annotation databases

    EnsemblBacteriaiCAE12886; CAE12886; plu0591.
    GeneIDi2800545.
    KEGGiplu:plu0591.
    PATRICi20504733. VBIPhoLum48522_0648.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571860 Genomic DNA. Translation: CAE12886.1 .
    RefSeqi NP_927939.1. NC_005126.1.

    3D structure databases

    ProteinModelPortali Q7N8X2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243265.plu0591.

    Proteomic databases

    PRIDEi Q7N8X2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE12886 ; CAE12886 ; plu0591 .
    GeneIDi 2800545.
    KEGGi plu:plu0591.
    PATRICi 20504733. VBIPhoLum48522_0648.

    Organism-specific databases

    GenoListi plu0591.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TT01.

    Entry informationi

    Entry nameiSYI_PHOLL
    AccessioniPrimary (citable) accession number: Q7N8X2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 15, 2003
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3