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Q7N8X2

- SYI_PHOLL

UniProt

Q7N8X2 - SYI_PHOLL

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Protein

Isoleucine--tRNA ligase

Gene
ileS, plu0591
Organism
Photorhabdus luminescens subsp. laumondii (strain TT01)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylate By similarity
Binding sitei605 – 6051ATP By similarity
Metal bindingi900 – 9001Zinc By similarity
Metal bindingi903 – 9031Zinc By similarity
Metal bindingi920 – 9201Zinc By similarity
Metal bindingi923 – 9231Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:plu0591
OrganismiPhotorhabdus luminescens subsp. laumondii (strain TT01)
Taxonomic identifieri243265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePhotorhabdus
ProteomesiUP000002514: Chromosome

Organism-specific databases

GenoListiplu0591.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 937937Isoleucine--tRNA ligaseUniRule annotationPRO_0000098438Add
BLAST

Proteomic databases

PRIDEiQ7N8X2.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi243265.plu0591.

Structurei

3D structure databases

ProteinModelPortaliQ7N8X2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi602 – 6065"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7N8X2-1 [UniParc]FASTAAdd to Basket

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MSDYKNTLNL PETGFPMRGD LAKREPNMLK RWYKDELYQV IRKAKAGKKT    50
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKGMAGYDSP YIPGWDCHGL 100
PIELKVEQII GKPGEKFSAA EFRAECRKYA KEQIEGQKKD FIRLGILGDW 150
ERPYLTMDFK TEADIIRALS RIIANGHLLK GAKPVHWCTD CRSSLAEAEV 200
EYYDKTSPSI DVRFNAVDAV AVCEKFGVQA PEQPVSLVIW TTTPWTLPAN 250
RAIALHAEFN YQLVQIEGEC LILAADLVES VMQRAGITSW TVLGHCAGSA 300
LELLRFKHPF MGFDSPVVLG DHVTLDAGTG AVHTAPGHGP DDFVLGQKYG 350
LEVANPVGPN GCYLPGTYPS LDGMFVFKAN DVVLNILSEN NALLHLEKLQ 400
HSYPCCWRHK TPIIFRATPQ WFVSMDQNGL RKQSLQEIKG VQWIPGWGQA 450
RIEAMVENRP DWCISRQRTW GTPMSLFVHK ETEELHPRTI ELMEEVAKRV 500
EVDGIQAWWD LEPAELLGDD AANYVKIFDT LDVWFDSGST HASVVDARPE 550
FQGNAADIYL EGSDQHRGWF MSSLMISTAI KGKAPYRQVL THGFTVDGQG 600
RKMSKSIGNT ISPQDVMDKL GADILRLWVA STDYTGEIAV SDEILKRSAD 650
AYRRIRNTAR FLLANLNGFD PEQHSVKPEE MAVLDRWAVG CAQAAQADIA 700
KCYDKYDFHT VVQRMMQFCS VEMGSFYLDI IKDRQYTAKS DSLARRSCQT 750
ALYHIAEALV RWMAPILSFT ADEVWNELPG KRAQYVFTEE WYGGLFGLAA 800
GELMNDAFWA DLLAVRGEVN KVLEQARADK HIRSSLEAAV TLYADNELAD 850
KLNSLGDELR FVLLTSQVVV ADYEQAGEDA QQSEIGSLKI AFRKADGEKC 900
PRCWHYAKDV GLVAEHAELC GRCVTNVAGN GEERKFA 937
Length:937
Mass (Da):104,977
Last modified:December 15, 2003 - v1
Checksum:i54EE6A4747B7DC1A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571860 Genomic DNA. Translation: CAE12886.1.
RefSeqiNP_927939.1. NC_005126.1.

Genome annotation databases

EnsemblBacteriaiCAE12886; CAE12886; plu0591.
GeneIDi2800545.
KEGGiplu:plu0591.
PATRICi20504733. VBIPhoLum48522_0648.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571860 Genomic DNA. Translation: CAE12886.1 .
RefSeqi NP_927939.1. NC_005126.1.

3D structure databases

ProteinModelPortali Q7N8X2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243265.plu0591.

Proteomic databases

PRIDEi Q7N8X2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE12886 ; CAE12886 ; plu0591 .
GeneIDi 2800545.
KEGGi plu:plu0591.
PATRICi 20504733. VBIPhoLum48522_0648.

Organism-specific databases

GenoListi plu0591.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TT01.

Entry informationi

Entry nameiSYI_PHOLL
AccessioniPrimary (citable) accession number: Q7N8X2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 15, 2003
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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