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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Photorhabdus luminescens subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei561Aminoacyl-adenylateUniRule annotation1
Binding sitei605ATPUniRule annotation1
Metal bindingi900ZincUniRule annotation1
Metal bindingi903ZincUniRule annotation1
Metal bindingi920ZincUniRule annotation1
Metal bindingi923ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPLUM243265:G1GTE-604-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:plu0591
OrganismiPhotorhabdus luminescens subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)
Taxonomic identifieri243265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesMorganellaceaePhotorhabdus
Proteomesi
  • UP000002514 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984381 – 937Isoleucine--tRNA ligaseAdd BLAST937

Proteomic databases

PRIDEiQ7N8X2

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243265.plu0591

Structurei

3D structure databases

ProteinModelPortaliQ7N8X2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi602 – 606"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q7N8X2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKNTLNL PETGFPMRGD LAKREPNMLK RWYKDELYQV IRKAKAGKKT
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKGMAGYDSP YIPGWDCHGL
110 120 130 140 150
PIELKVEQII GKPGEKFSAA EFRAECRKYA KEQIEGQKKD FIRLGILGDW
160 170 180 190 200
ERPYLTMDFK TEADIIRALS RIIANGHLLK GAKPVHWCTD CRSSLAEAEV
210 220 230 240 250
EYYDKTSPSI DVRFNAVDAV AVCEKFGVQA PEQPVSLVIW TTTPWTLPAN
260 270 280 290 300
RAIALHAEFN YQLVQIEGEC LILAADLVES VMQRAGITSW TVLGHCAGSA
310 320 330 340 350
LELLRFKHPF MGFDSPVVLG DHVTLDAGTG AVHTAPGHGP DDFVLGQKYG
360 370 380 390 400
LEVANPVGPN GCYLPGTYPS LDGMFVFKAN DVVLNILSEN NALLHLEKLQ
410 420 430 440 450
HSYPCCWRHK TPIIFRATPQ WFVSMDQNGL RKQSLQEIKG VQWIPGWGQA
460 470 480 490 500
RIEAMVENRP DWCISRQRTW GTPMSLFVHK ETEELHPRTI ELMEEVAKRV
510 520 530 540 550
EVDGIQAWWD LEPAELLGDD AANYVKIFDT LDVWFDSGST HASVVDARPE
560 570 580 590 600
FQGNAADIYL EGSDQHRGWF MSSLMISTAI KGKAPYRQVL THGFTVDGQG
610 620 630 640 650
RKMSKSIGNT ISPQDVMDKL GADILRLWVA STDYTGEIAV SDEILKRSAD
660 670 680 690 700
AYRRIRNTAR FLLANLNGFD PEQHSVKPEE MAVLDRWAVG CAQAAQADIA
710 720 730 740 750
KCYDKYDFHT VVQRMMQFCS VEMGSFYLDI IKDRQYTAKS DSLARRSCQT
760 770 780 790 800
ALYHIAEALV RWMAPILSFT ADEVWNELPG KRAQYVFTEE WYGGLFGLAA
810 820 830 840 850
GELMNDAFWA DLLAVRGEVN KVLEQARADK HIRSSLEAAV TLYADNELAD
860 870 880 890 900
KLNSLGDELR FVLLTSQVVV ADYEQAGEDA QQSEIGSLKI AFRKADGEKC
910 920 930
PRCWHYAKDV GLVAEHAELC GRCVTNVAGN GEERKFA
Length:937
Mass (Da):104,977
Last modified:December 15, 2003 - v1
Checksum:i54EE6A4747B7DC1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571860 Genomic DNA Translation: CAE12886.1
RefSeqiWP_011144970.1, NC_005126.1

Genome annotation databases

EnsemblBacteriaiCAE12886; CAE12886; plu0591
KEGGiplu:plu0591

Similar proteinsi

Entry informationi

Entry nameiSYI_PHOLL
AccessioniPrimary (citable) accession number: Q7N8X2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 15, 2003
Last modified: March 28, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health