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Q7N8P9

- GLND_PHOLL

UniProt

Q7N8P9 - GLND_PHOLL

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, plu0670
Organism
Photorhabdus luminescens subsp. laumondii (strain TT01)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:plu0670
OrganismiPhotorhabdus luminescens subsp. laumondii (strain TT01)
Taxonomic identifieri243265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePhotorhabdus
ProteomesiUP000002514: Chromosome

Organism-specific databases

GenoListiplu0670.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 882882Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_0000192751Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243265.plu0670.

Structurei

3D structure databases

ProteinModelPortaliQ7N8P9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini462 – 595134HD
Add
BLAST
Domaini701 – 77878ACT 1
Add
BLAST
Domaini808 – 88275ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 343343UridylyltransferaseUniRule annotation
Add
BLAST
Regioni344 – 700357Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7N8P9-1 [UniParc]FASTAAdd to Basket

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MSADIATIQA PIPPLSPADF SSEDLRYPVL KQHLEEFQLW LEHAFKAGIS    50
AEALISARSD YIDQLLQQLW YAYRFDKISS LSLIAVGGYG RRELHPLSDI 100
DVLILSEQPL TPPQAQNVGQ FITLLWDIRL EVGHSVRTLE ECLLEGLSDL 150
TIATNLIESR LICGDSSIFL RLQRHTFSDG FWPSTEFFDA KIVEQHERHQ 200
RYHSTSYNLE PDIKSSPGGL RDIHTLLWVA RRHFGATSID EMVDFGFLTA 250
EERNELNECQ SFLWRIRFAL HLVVNRYDNR LLFDRQFSIA QLLGYHGERN 300
QPVERMMKDF YRMTRRVSEL NNMLLQLFDE AILALETNEK SRSLDSEFQL 350
RGQLIDLIDE TLFIKEPAAI MRMFYRMAEH EEVQGIYSTT LRHLRYARRN 400
LSQPLCELPE ARQIFMDILR HPRAVESAFV PMHRHSVLGA YTPLWGNIVG 450
QMQFDLFHAY TVDEHTIRVL RKLESFANEN NRPAHPLCVE LYPRLPQPEL 500
LHLAALFHDI AKGRTGDHSE LGADDALAFS LKHGLNSREA DLVAWLVRHH 550
LLMSVTAQRR DIQDPEIIKQ FTHQILNETR LRYLICLTVA DICATNVNLW 600
NSWKQSLLRE LYFSTENQLR QGNTPDFRER IRHNRFQALA LLRQDNINEQ 650
KLHQLWSRCH ADYFLRHTPK QLAWHAHHLV QHDSQESLIL ISTKPTRGGT 700
EIFIWSVDRP SLFAAVVGEL DRRNLSVHHA QIFTNRDGMT MDTFVVLEPN 750
GHPLASDRHE IIRNALLQVV LAPHTKTPKT RKLPTKLRHF NVPTKVTFLP 800
THNERRTYME LFALDQPGLL ARVGNIFAEM GVSLHGAHIT TIGERVEDFF 850
VLADKDHKAL NKKVREELSE RLTATLNPKD KI 882
Length:882
Mass (Da):102,232
Last modified:December 15, 2003 - v1
Checksum:i12FEA798C315CBCD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571861 Genomic DNA. Translation: CAE12965.1.
RefSeqiNP_928015.1. NC_005126.1.
WP_011145046.1. NC_005126.1.

Genome annotation databases

EnsemblBacteriaiCAE12965; CAE12965; plu0670.
GeneIDi2800627.
KEGGiplu:plu0670.
PATRICi20504919. VBIPhoLum48522_0738.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571861 Genomic DNA. Translation: CAE12965.1 .
RefSeqi NP_928015.1. NC_005126.1.
WP_011145046.1. NC_005126.1.

3D structure databases

ProteinModelPortali Q7N8P9.
ModBasei Search...

Protein-protein interaction databases

STRINGi 243265.plu0670.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE12965 ; CAE12965 ; plu0670 .
GeneIDi 2800627.
KEGGi plu:plu0670.
PATRICi 20504919. VBIPhoLum48522_0738.

Organism-specific databases

GenoListi plu0670.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TT01.

Entry informationi

Entry nameiGLND_PHOLL
AccessioniPrimary (citable) accession number: Q7N8P9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: December 15, 2003
Last modified: September 3, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi