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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Photorhabdus luminescens subsp. laumondii (strain TT01)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:plu0670
OrganismiPhotorhabdus luminescens subsp. laumondii (strain TT01)
Taxonomic identifieri243265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePhotorhabdus
ProteomesiUP000002514 Componenti: Chromosome

Organism-specific databases

GenoListiplu0670.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 882882Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192751Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243265.plu0670.

Structurei

3D structure databases

ProteinModelPortaliQ7N8P9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini462 – 595134HDUniRule annotationAdd
BLAST
Domaini701 – 77878ACT 1UniRule annotationAdd
BLAST
Domaini808 – 88275ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 343343UridylyltransferaseAdd
BLAST
Regioni344 – 700357Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiSTIGERV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7N8P9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSADIATIQA PIPPLSPADF SSEDLRYPVL KQHLEEFQLW LEHAFKAGIS
60 70 80 90 100
AEALISARSD YIDQLLQQLW YAYRFDKISS LSLIAVGGYG RRELHPLSDI
110 120 130 140 150
DVLILSEQPL TPPQAQNVGQ FITLLWDIRL EVGHSVRTLE ECLLEGLSDL
160 170 180 190 200
TIATNLIESR LICGDSSIFL RLQRHTFSDG FWPSTEFFDA KIVEQHERHQ
210 220 230 240 250
RYHSTSYNLE PDIKSSPGGL RDIHTLLWVA RRHFGATSID EMVDFGFLTA
260 270 280 290 300
EERNELNECQ SFLWRIRFAL HLVVNRYDNR LLFDRQFSIA QLLGYHGERN
310 320 330 340 350
QPVERMMKDF YRMTRRVSEL NNMLLQLFDE AILALETNEK SRSLDSEFQL
360 370 380 390 400
RGQLIDLIDE TLFIKEPAAI MRMFYRMAEH EEVQGIYSTT LRHLRYARRN
410 420 430 440 450
LSQPLCELPE ARQIFMDILR HPRAVESAFV PMHRHSVLGA YTPLWGNIVG
460 470 480 490 500
QMQFDLFHAY TVDEHTIRVL RKLESFANEN NRPAHPLCVE LYPRLPQPEL
510 520 530 540 550
LHLAALFHDI AKGRTGDHSE LGADDALAFS LKHGLNSREA DLVAWLVRHH
560 570 580 590 600
LLMSVTAQRR DIQDPEIIKQ FTHQILNETR LRYLICLTVA DICATNVNLW
610 620 630 640 650
NSWKQSLLRE LYFSTENQLR QGNTPDFRER IRHNRFQALA LLRQDNINEQ
660 670 680 690 700
KLHQLWSRCH ADYFLRHTPK QLAWHAHHLV QHDSQESLIL ISTKPTRGGT
710 720 730 740 750
EIFIWSVDRP SLFAAVVGEL DRRNLSVHHA QIFTNRDGMT MDTFVVLEPN
760 770 780 790 800
GHPLASDRHE IIRNALLQVV LAPHTKTPKT RKLPTKLRHF NVPTKVTFLP
810 820 830 840 850
THNERRTYME LFALDQPGLL ARVGNIFAEM GVSLHGAHIT TIGERVEDFF
860 870 880
VLADKDHKAL NKKVREELSE RLTATLNPKD KI
Length:882
Mass (Da):102,232
Last modified:December 15, 2003 - v1
Checksum:i12FEA798C315CBCD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571861 Genomic DNA. Translation: CAE12965.1.
RefSeqiNP_928015.1. NC_005126.1.

Genome annotation databases

KEGGiplu:plu0670.
PATRICi20504919. VBIPhoLum48522_0738.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571861 Genomic DNA. Translation: CAE12965.1.
RefSeqiNP_928015.1. NC_005126.1.

3D structure databases

ProteinModelPortaliQ7N8P9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243265.plu0670.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiplu:plu0670.
PATRICi20504919. VBIPhoLum48522_0738.

Organism-specific databases

GenoListiplu0670.

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiSTIGERV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TT01.

Entry informationi

Entry nameiGLND_PHOLL
AccessioniPrimary (citable) accession number: Q7N8P9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: December 15, 2003
Last modified: April 1, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.