Q7N8N0 (TILS_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA(Ile)-lysidine synthase
EC=6.3.4.19

Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase
tRNA(Ile)-lysidine synthetase

Gene names

Name:	tilS
Ordered Locus Names:	plu0689

Organism

Photorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]

Taxonomic identifier

243265 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus › Photorhabdus luminescens › Photorhabdus luminescens subsp. laumondii

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine By similarity. HAMAP-Rule MF_01161
Catalytic activity	(tRNA(Ile2))-cytidine34 + L-lysine + ATP = (tRNA(Ile2))-lysidine34 + AMP + diphosphate + H2O. HAMAP-Rule MF_01161
Subcellular location	Cytoplasm By similarity.
Domain	The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. HAMAP-Rule MF_01161
Sequence similarities	Belongs to the tRNA(Ile)-lysidine synthase family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP ligase activity, forming carbon-nitrogen bonds Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	tRNA(Ile)-lysidine synthase HAMAP-Rule MF_01161		PRO_0000181741
Regions
	Nucleotide binding	27 – 32	6	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7N8N0 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 06DB75D273109DC6	FASTA	442	51,163
	10 20 30 40 50 60 MANIDERLFV TLAKQLGEYK KILVGFSGGL DSSVLLHLLV NWRNQHNQKI QLRAIHIHHG 70 80 90 100 110 120 LNLKADQWIE HCQLICDDWQ VEFHSARVTI DARQKGIEAA ARDARYQVFK SELQKDEVLV 130 140 150 160 170 180 TAQHLDDQAE TFLLALKRGS GPAGLASMPS IAVFADTLLL RPLLDYSRNE LEKYASKHRL 190 200 210 220 230 240 NWIEDDSNQD DRYDRNFLRL HIMPLLNQRW SHFPKAAARS ASLCGEQEQL LDELLNESLK 250 260 270 280 290 300 ELITQDGALG IVSLAVCSEA KRNALLRRWF GYHGMKMPSR EQLNRLWYEV ALARTDAEPR 310 320 330 340 350 360 LQFGKYDVRR YKQKLWLVPQ WQSLRETILE WDICNTLVLP DRLGELNIAD RGIQVRVPAD 370 380 390 400 410 420 NERVTIRFGV QGMISIVGRQ HSRHSKKLWQ ELGVAPWLRE RIPLLYYNEQ LIAALGVFVT 430 440 KESEAAEGRK MLTMNWHKTL LK

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571861 Genomic DNA. Translation: CAE12984.1.
RefSeq	NP_928034.1. NC_005126.1.
3D structure databases
ProteinModelPortal	Q7N8N0.
SMR	Q7N8N0. Positions 9-440.
ModBase	Search...
Protein-protein interaction databases
STRING	243265.plu0689.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE12984; CAE12984; plu0689.
GeneID	2800646.
KEGG	plu:plu0689.
PATRIC	20504963. VBIPhoLum48522_0760.
Organism-specific databases
GenoList	plu0689.
CMR	Search...
Phylogenomic databases
eggNOG	COG0037.
HOGENOM	HOG000236507.
KO	K04075.
OMA	SNQDDAY.
ProtClustDB	PRK10660.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_01161. tRNA(Ile)-lys_synt.
InterPro	IPR012094. Lysidine-tRNA-synth. IPR012796. Lysidine-tRNA-synth_C. IPR012795. Lysidine-tRNA-synth_N. IPR015262. Lysidine-tRNA-synth_subst-bd. IPR020825. Phe-tRNA_synthase_B3/B4. IPR014729. Rossmann-like_a/b/a_fold. IPR011063. tRNA-lysidine/thiocyt_synth. [Graphical view]
PANTHER	PTHR11807:SF2. PTHR11807:SF2. 1 hit.
Pfam	PF01171. ATP_bind_3. 1 hit. PF09179. TilS. 1 hit. PF11734. TilS_C. 1 hit. [Graphical view]
SMART	SM00977. TilS_C. 1 hit. [Graphical view]
SUPFAM	SSF56037. B3_4. 1 hit.
TIGRFAMs	TIGR02433. lysidine_TilS_C. 1 hit. TIGR02432. lysidine_TilS_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TILS_PHOLL

Accession

Primary (citable) accession number: Q7N8N0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program