Reviewed, UniProtKB/Swiss-Prot Q7N8L5 (CYSI_PHOLL)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	plu0704

Organism

Photorhabdus luminescens subsp. laumondii [Complete proteome] [HAMAP]

Taxonomic identifier

141679 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH.
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Sulfite reductase [NADPH] hemoprotein beta-component		PRO_0000199903
Sites
	Metal binding	435	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	441	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	480	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	484	1	Iron (siroheme axial ligand) By similarity
	Metal binding	484	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7N8L5-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 3CA6B1166715B15D	FASTA	576	64,829
	10 20 30 40 50 60 MNDKQHGPFI VEGKLADSER MKQESNFLRG TISEDLTNGL TGGFEGDNFL LIRFHGMYQQ 70 80 90 100 110 120 DDRDIRAERA EQKLEPRHAI MLRCRLPGGV ITPQQWLGID KFAEENTLYG SIRLTNRQTF 130 140 150 160 170 180 QFHGILKGKV KLAHQLLNQV GLDSLATAND VNRNVLCTSN PIQSELHQQA YEWAKKISEH 190 200 210 220 230 240 LLPRTRAYAE LWLDKEKVAT TDEEPILGST YLPRKFKTTV VIPPQNDVDL HANDLNFIAI 250 260 270 280 290 300 AEGSKLVGFN VLVGGGLAMT HGDKNTYPSM ASEFGYIPLE HTLAIAEAVV TTQRDWGNRT 310 320 330 340 350 360 ERKNAKTKYT LERVGVETFK AEVERRAGVK FEAIRPYEFT GRGDQIGWLK GIDDKWHLTL 370 380 390 400 410 420 FIENGRLLDY PGKPLKSGVA EIAKIHKGDF RLTANQNLII AGIPESEKQR IETIACRHGL 430 440 450 460 470 480 IDDKTTVQRK NSMACVSFPT CPLAMAEAER FLPEFVTHVE QLMNKHGIGD EHIVLRVTGC 490 500 510 520 530 540 PNGCARAMLA EVGLVGKALD RYNLHLGGNR IGTRIPRMYK ENISSQAILS IMDELIGRWA 550 560 570 TGRQPNEGFG DFLIRTDIIK PVLDSARDFY DWQEAV

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571861 Genomic DNA. Translation: CAE12999.1.
RefSeq	NP_928049.1.
3D structure databases
HSSP	HSSP built from PDB template 1AOP based on UniProtKB P17846.
SMR	Q7N8L5. Positions 81-571.
ModBase	Search...
Genome annotation databases
GeneID	2800667.
GenomeReviews	Gene locus plu0704 in contig BX470251_GR.
KEGG	plu:plu0704.
NMPDR	fig|243265.1.peg.673.
Organism-specific databases
PhotoList	plu0704.
CMR	Search...
Phylogenomic databases
HOGENOM	Q7N8L5.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU0704-MON.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_PHOLL

Accession

Primary (citable) accession number: Q7N8L5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 15, 2003
Last modified:	November 25, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)