Siroheme synthase - Photorhabdus luminescens subsp. laumondii

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Reviewed, UniProtKB/Swiss-Prot Q7N8L2 (CYSG_PHOLL)

Last modified February 9, 2010. Version 42. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Siroheme synthase
Including the following 3 domains:
    1- Recommended name:
            Uroporphyrinogen-III C-methyltransferase
                Short name=Urogen III methylase
              EC=2.1.1.107
        Alternative name(s):
            SUMT
            Uroporphyrinogen III methylase
              Short name=UROM
    2- Recommended name:
            Precorrin-2 dehydrogenase
              EC=1.3.1.76
    3- Recommended name:
            Sirohydrochlorin ferrochelatase
              EC=4.99.1.4

Gene names

Name:	cysG
Ordered Locus Names:	plu0708

Organism

Photorhabdus luminescens subsp. laumondii [Complete proteome] [HAMAP]

Taxonomic identifier

141679 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

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Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme By similarity. HAMAP MF_01646
Catalytic activity	S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. HAMAP MF_01646 S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. HAMAP MF_01646 Precorrin-2 + NAD⁺ = sirohydrochlorin + NADH. HAMAP MF_01646 Siroheme + 2 H⁺ = sirohydrochlorin + Fe²⁺. HAMAP MF_01646
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646 Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1. Porphyrin metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646 Porphyrin metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1. HAMAP MF_01646 Porphyrin metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Sequence similarities	Belongs to the precorrin methyltransferase family.

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Ontologies

Keywords
Biological process	Cobalamin biosynthesis Porphyrin biosynthesis
Ligand	NAD S-adenosyl-L-methionine
Molecular function	Lyase Methyltransferase Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW siroheme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro precorrin-2 dehydrogenase activity Inferred from electronic annotation. Source: HAMAP sirohydrochlorin ferrochelatase activity Inferred from electronic annotation. Source: EC uroporphyrin-III C-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 470

470

Siroheme synthase HAMAP MF_01646

PRO_0000330530

Regions

Region

216 – 456

241

Uroporphyrinogen-III C-methyltransferase HAMAP MF_01646

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Sequences

Sequence

Length

Mass (Da)

Tools

Q7N8L2-1 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 343EAAB8BF73706F
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FASTA

470

51,343

        10         20         30         40         50         60 
MDYLPIFVEL KGRLVLLVGG GEVAARKATL LLRAGALLQV VAPELCSELQ QRYQAGELEW 

        70         80         90        100        110        120 
YQGEFQPEYL DGIFLVIAAT DDRILNHQVF SEADKRSILV NVVDDQVHCS FIFPSIIDRS 

       130        140        150        160        170        180 
PVLVAISSAG KAPVLARLIR EKLEALLPSS LGTMAKIAGK WRERVKQRLT SMRQRRSFWE 

       190        200        210        220        230        240 
QAFNGRFAML VANGQIQQAE KQLEQQLEQS DLQGELALVG AGPGDPGLLT LKGLQVIQQA 

       250        260        270        280        290        300 
DVVLYDHLVS SDVLDLIRRD ADKICVGKRA GNHSVSQEET NRLIVKFARQ GKKVVRLKGG 

       310        320        330        340        350        360 
DPFIFGRGGE ELQVAASAGI PFQVVPGITA AIGATAYAGI PLTHREHSQS ITFITGHCRE 

       370        380        390        400        410        420 
KGNELDWPAL ARGHQTLVIY MGTVKAALIS HQLILHGRAE DTPVAVIGCG TRLEQQVLTG 

       430        440        450        460        470 
TLLELEQLAQ QAPSPALLVV GEVAQLHHQI AWFGQQSIAK ISRPAVVDFA

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References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens."
Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K.

Kunst F.
Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TT01.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571861 Genomic DNA. Translation: CAE13003.1.
RefSeq	NP_928053.1.
3D structure databases
HSSP	HSSP built from PDB template 1PJS based on UniProtKB P25924.
SMR	Q7N8L2. Positions 1-455.
ModBase	Search...
Genome annotation databases
GeneID	2800671.
KEGG	plu:plu0708.
NMPDR	fig\|243265.1.peg.677.
Organism-specific databases
PhotoList	plu0708.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG730212.
OMA	AWTPATG.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU0708-MONOMER.
Family and domain databases
HAMAP	MF_01646. Siroheme_synth. [Tree]
InterPro	IPR000878. 4pyrrol_Mease. IPR014777. 4pyrrole_Mease_sub1. IPR014776. 4pyrrole_Mease_sub2. IPR006366. CobA_cysG_C. IPR016040. NAD(P)-bd_dom. IPR019478. Sirohaem_synthase_dimer_dom. IPR006367. Sirohaem_synthase_N. IPR003043. Uropor_MeTrfase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1010.10. 4pyrrole_Mease_sub1. 1 hit. G3DSA:3.30.950.10. 4pyrrole_Mease_sub2. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF10414. CysG_dimeriser. 1 hit. PF00590. TP_methylase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01469. cobA_cysG_Cterm. 1 hit. TIGR01470. cysG_Nterm. 1 hit.
PROSITE	PS00839. SUMT_1. 1 hit. PS00840. SUMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

CYSG_PHOLL

Accession

Primary (citable) accession number: Q7N8L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	December 15, 2003
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents