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Q7N8L2 (CYSG_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Siroheme synthase

Including the following 3 domains:

  1. Uroporphyrinogen-III C-methyltransferase
    Short name=Urogen III methylase
    EC=2.1.1.107
    Alternative name(s):
    SUMT
    Uroporphyrinogen III methylase
    Short name=UROM
  2. Precorrin-2 dehydrogenase
    EC=1.3.1.76
  3. Sirohydrochlorin ferrochelatase
    EC=4.99.1.4
Gene names
Name:cysG
Ordered Locus Names:plu0708
OrganismPhotorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]
Taxonomic identifier243265 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme By similarity. HAMAP MF_01646

Catalytic activity

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. HAMAP MF_01646

S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. HAMAP MF_01646

Precorrin-2 + NAD+ = sirohydrochlorin + NADH. HAMAP MF_01646

Siroheme + 2 H+ = sirohydrochlorin + Fe2+. HAMAP MF_01646

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646

Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Porphyrin metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646

Porphyrin metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1. HAMAP MF_01646

Porphyrin metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Sequence similarities

Belongs to the precorrin methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 470470Siroheme synthase HAMAP MF_01646
PRO_0000330530

Regions

Region216 – 456241Uroporphyrinogen-III C-methyltransferase HAMAP MF_01646

Sequences

Sequence LengthMass (Da)Tools
Q7N8L2 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 343EAAB8BF73706F

FASTA47051,343
        10         20         30         40         50         60 
MDYLPIFVEL KGRLVLLVGG GEVAARKATL LLRAGALLQV VAPELCSELQ QRYQAGELEW 

        70         80         90        100        110        120 
YQGEFQPEYL DGIFLVIAAT DDRILNHQVF SEADKRSILV NVVDDQVHCS FIFPSIIDRS 

       130        140        150        160        170        180 
PVLVAISSAG KAPVLARLIR EKLEALLPSS LGTMAKIAGK WRERVKQRLT SMRQRRSFWE 

       190        200        210        220        230        240 
QAFNGRFAML VANGQIQQAE KQLEQQLEQS DLQGELALVG AGPGDPGLLT LKGLQVIQQA 

       250        260        270        280        290        300 
DVVLYDHLVS SDVLDLIRRD ADKICVGKRA GNHSVSQEET NRLIVKFARQ GKKVVRLKGG 

       310        320        330        340        350        360 
DPFIFGRGGE ELQVAASAGI PFQVVPGITA AIGATAYAGI PLTHREHSQS ITFITGHCRE 

       370        380        390        400        410        420 
KGNELDWPAL ARGHQTLVIY MGTVKAALIS HQLILHGRAE DTPVAVIGCG TRLEQQVLTG 

       430        440        450        460        470 
TLLELEQLAQ QAPSPALLVV GEVAQLHHQI AWFGQQSIAK ISRPAVVDFA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX571861 Genomic DNA. Translation: CAE13003.1.
RefSeqNP_928053.1. NC_005126.1.

3D structure databases

HSSPHSSP built from PDB template 1PJS based on UniProtKB P25924.
ProteinModelPortalQ7N8L2.
SMRQ7N8L2. Positions 1-455.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2800671.
GenomeReviewsGene locus plu0708 in contig BX470251_GR.
KEGGplu:plu0708.
NMPDRfig|243265.1.peg.677.
PATRIC20505013. VBIPhoLum48522_0779.

Organism-specific databases

GenoListplu0708.
CMRSearch...

Phylogenomic databases

HOGENOMHBG730212.
OMADYAQSAV.
PhylomeDBQ7N8L2.
ProtClustDBCLSK2521698.

Enzyme and pathway databases

BioCycPLUM243265:PLU0708-MONOMER.

Family and domain databases

HAMAPMF_01646. Siroheme_synth.
[Tree]
InterProIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA_cysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
Gene3DG3DSA:3.40.1010.10. 4pyrrole_Mease_sub1. 1 hit.
G3DSA:3.30.950.10. 4pyrrole_Mease_sub2. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
G3DSA:1.10.8.210. Sirohaem_synthase_dimer_dom. 1 hit.
KOK02302.
PfamPF10414. CysG_dimeriser. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMSSF53790. Cor/por_Metransf. 1 hit.
TIGRFAMsTIGR01469. CobA_cysG_Cterm. 1 hit.
TIGR01470. CysG_Nterm. 1 hit.
PROSITEPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSG_PHOLL
AccessionPrimary (citable) accession number: Q7N8L2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 15, 2003
Last modified: January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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