Reviewed, UniProtKB/Swiss-Prot Q7N8K3 (PIMT_PHOLL)

Names and origin

Protein names

Recommended name:
    Protein-L-isoaspartate O-methyltransferase
    EC=2.1.1.77
Alternative name(s):
    Protein-beta-aspartate methyltransferase
      Short name=PIMT
    Protein L-isoaspartyl methyltransferase
    L-isoaspartyl protein carboxyl methyltransferase

Gene names

Name:	pcm
Ordered Locus Names:	plu0717

Organism

Photorhabdus luminescens subsp. laumondii [Complete proteome] [HAMAP]

Taxonomic identifier

141679 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity.
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester. HAMAP MF_00090
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: HAMAP protein repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Protein-L-isoaspartate O-methyltransferase HAMAP MF_00090		PRO_0000111895
Sites
	Active site	59	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7N8K3-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 5223D7B1A6FB66A4	FASTA	208	23,157
	10 20 30 40 50 60 MLSRAMKNLL TQLRQQGIED ERLLAAISAV PRERFVDEAL AHKAYENTAL PIGYGQTISQ 70 80 90 100 110 120 PYIVARMTEL LQLTPDAKIL EIGTGSGYQT AILAHLVKHV FSVERIKGLQ WQAKRRLKQL 130 140 150 160 170 180 DLHNVSTRHG DGWQGWPSRG LFDAIIVTAA PPYIPQELML QLTDGGVMVL PVGEHTQILK 190 200 SVKRHGNGFH SEVIEAVRFV PLVQGELA

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571861 Genomic DNA. Translation: CAE13012.1.
RefSeq	NP_928062.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2800680.
GenomeReviews	Gene locus plu0717 in contig BX470251_GR.
KEGG	plu:plu0717.
NMPDR	fig|243265.1.peg.686.
Organism-specific databases
PhotoList	plu0717.
CMR	Search...
Phylogenomic databases
HOGENOM	Q7N8K3.
OMA	Q7N8K3. KELNYAN.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU0717-MON.
BRENDA	2.1.1.77. 308689.
Family and domain databases
HAMAP	MF_00090. [Tree]
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PCMT. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT_PHOLL

Accession

Primary (citable) accession number: Q7N8K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)