Reviewed, UniProtKB/Swiss-Prot Q7N8D4 (PURA1_PHOLL)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase 1
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase 1
    AdSS 1
    AMPSase 1

Gene names

Name:	purA1
Ordered Locus Names:	plu0794

Organism

Photorhabdus luminescens subsp. laumondii [Complete proteome] [HAMAP]

Taxonomic identifier

141679 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Adenylosuccinate synthetase 1 HAMAP MF_00011		PRO_0000095207
Regions
	Nucleotide binding	11 – 17	7	GTP Potential
Sites
	Active site	135	1	By similarity
	Active site	142	1	By similarity
	Metal binding	12	1	Magnesium By similarity
	Metal binding	39	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7N8D4-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 7191F44A4E238351	FASTA	419	46,976
	10 20 30 40 50 60 MLTAVVGLQF GDEGKGKFVD YLSGNINNIA RFNGGANAGH CVQHGNLRGS FSQLPASLNK 70 80 90 100 110 120 KNLYICQGAL ISLPILVKEI DFIKKEDIDS HIFIDPRCHI VLPLHAELNR ASEKYKGKQK 130 140 150 160 170 180 IGSVGVGVGA CVEDKANRHG IRLIDTFDKE KLRSKLEFLW SIREKQINHV FNAQASLDFE 190 200 210 220 230 240 EMLETTHQYG KRIEPYFTFT NEIIGDLLNS GEDVLLETSQ ATFLDNSFGT YPYTVAYQTL 250 260 270 280 290 300 VQTCFAMIGI PAQKMHIVGV MKSYMIRVGN GPFPTELSTE QADYIRERGN EYGTVSKRPR 310 320 330 340 350 360 RCGWLDLSLI KHAVKLNGVI ELAITNVDVL AGLDEIKVAV AYEIDDKLVC CDNALLQLDR 370 380 390 400 410 AKPIYKTFKS WSTLNSSYTD LTELPIELID FLSFIQDYTG VPIKYISYGP DRNQTLVVK

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571861 Genomic DNA. Translation: CAE13089.1.
RefSeq	NP_928139.1.
3D structure databases
SMR	Q7N8D4. Positions 1-417.
ModBase	Search...
Genome annotation databases
GeneID	2800757.
KEGG	plu:plu0794.
NMPDR	fig|243265.1.peg.763.
Organism-specific databases
PhotoList	plu0794.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG658237.
OMA	KIAITHI.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU0794-MONOMER.
BRENDA	6.3.4.4. 308689.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA1_PHOLL

Accession

Primary (citable) accession number: Q7N8D4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	December 15, 2003
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)