Q7N892 (SPEE_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermidine synthase
EC=2.5.1.16

Alternative name(s):
Putrescine aminopropyltransferase
Short name=PAPT
SPDSY

Gene names

Name:	speE
Ordered Locus Names:	plu0843

Organism

Photorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]

Taxonomic identifier

243265 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus › Photorhabdus luminescens › Photorhabdus luminescens subsp. laumondii

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor By similarity. HAMAP-Rule MF_00198
Catalytic activity	S-adenosylmethioninamine + putrescine = S-methyl-5'-thioadenosine + spermidine. HAMAP-Rule MF_00198
Pathway	Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1. HAMAP-Rule MF_00198
Subunit structure	Homodimer and homotetramer By similarity.
Sequence similarities	Belongs to the spermidine/spermine synthase family.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Spermidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	spermidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	spermidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Spermidine synthase HAMAP-Rule MF_00198		PRO_0000156493
Regions
	Region	140 – 141	2	S-adenosylmethioninamine binding By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	33	1	S-adenosylmethioninamine By similarity
	Binding site	63	1	Putrescine By similarity
	Binding site	88	1	S-adenosylmethioninamine By similarity
	Binding site	108	1	S-adenosylmethioninamine By similarity
	Binding site	161	1	Putrescine By similarity
	Binding site	226	1	Putrescine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7N892 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 95411BCC84EF109C	FASTA	289	32,483
	10 20 30 40 50 60 MSQKEIWSET LHANFGQYFS VDKVLYHDKT EHQDLIIFEN ATLGRIMALD GTVQTTERDE 70 80 90 100 110 120 FIYHEMMAHV PLFAHGNAKR VLIIGGGDGG MLREVCRHQY LESITMVEID AGVVEFCRQY 130 140 150 160 170 180 LPNHNAGAYD DPRFNLIIDD GVNFVNTTDQ KFDVIISDCT DPIGPGEGLF TSEFYQGCQR 190 200 210 220 230 240 CLNEGGIFVA QNGVCFLQQD EAVTSHKKLS NYFADSGFYQ AAIPTYYGGI MTFAWASQNP 250 260 270 280 ALRHVDLATL QQRFDNASFT CRYYNPAVHV GSFALPQYLL DALFTGCTI

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571861 Genomic DNA. Translation: CAE13138.1.
RefSeq	NP_928186.1. NC_005126.1.
3D structure databases
ProteinModelPortal	Q7N892.
ModBase	Search...
Protein-protein interaction databases
STRING	243265.plu0843.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE13138; CAE13138; plu0843.
GeneID	2800806.
KEGG	plu:plu0843.
PATRIC	20505321. VBIPhoLum48522_0934.
Organism-specific databases
GenoList	plu0843.
CMR	Search...
Phylogenomic databases
eggNOG	COG0421.
HOGENOM	HOG000256146.
KO	K00797.
OMA	ELWYTEK.
ProtClustDB	PRK00811.
Enzyme and pathway databases
UniPathway	UPA00248; UER00314.
Family and domain databases
HAMAP	MF_00198. Spermidine_synth.
InterPro	IPR001045. Spermidine/spermine_synthase. [Graphical view]
PANTHER	PTHR11558. PTHR11558. 1 hit.
Pfam	PF01564. Spermine_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00417. speE. 1 hit.
PROSITE	PS01330. SPERMIDINE_SYNTHASE_1. 1 hit. PS51006. SPERMIDINE_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEE_PHOLL

Accession

Primary (citable) accession number: Q7N892

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program