Q7N809 (GLO2_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	plu0940

Organism

Photorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]

Taxonomic identifier

243265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_0000309673
Sites
	Metal binding	53	1	Zinc 1 By similarity
	Metal binding	55	1	Zinc 1 By similarity
	Metal binding	57	1	Zinc 2 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	110	1	Zinc 1 By similarity
	Metal binding	127	1	Zinc 1 By similarity
	Metal binding	127	1	Zinc 2 By similarity
	Metal binding	165	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7N809 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 02B3B3641DEC52E2	FASTA	250	27,984
	10 20 30 40 50 60 MELISIPALS DNYIWLLCDE NRHVVIVDPA ESQPVLDTLE SRGWIPDAIL LTHHHHDHVG 70 80 90 100 110 120 GVPGIIDRFP ELLVYGPAET QSKGATIIVC GGDSINFGHF SFQIIDVPGH TLGHIAFYQA 130 140 150 160 170 180 PYLFCGDTLF SGGCGRLFEG TAEQMYTSIG KIAELPDDTL ICCAHEYTIS NMKFAHAVLP 190 200 210 220 230 240 NQTISEYQQK VIRMRENNQP TIPTTLQTEK KINIFLKCDD IDLQRNIGIT PNSQPLSAVF 250 HQLRRQKDQF

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571862 Genomic DNA. Translation: CAE13235.1.
RefSeq	NP_928276.1. NC_005126.1.
3D structure databases
HSSP	HSSP built from PDB template 1XM8 based on UniProtKB Q9SID3.
ProteinModelPortal	Q7N809.
SMR	Q7N809. Positions 1-250.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2800903.
GenomeReviews	Gene locus plu0940 in contig BX470251_GR.
KEGG	plu:plu0940.
NMPDR	fig|243265.1.peg.900.
PATRIC	20505527. VBIPhoLum48522_1037.
Organism-specific databases
GenoList	plu0940.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753931.
OMA	FLCRTDN.
PhylomeDB	Q7N809.
ProtClustDB	CLSK2521726.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU0940-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_PHOLL

Accession

Primary (citable) accession number: Q7N809

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program