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Q7N7B2 (PROB_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate 5-kinase

EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase
Short name=GK
Gene names
Name:proB
Ordered Locus Names:plu1243
OrganismPhotorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]
Taxonomic identifier243265 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePhotorhabdus

Protein attributes

Sequence length368 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00456.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: InterPro

glutamate 5-kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 368368Glutamate 5-kinase HAMAP-Rule MF_00456
PRO_0000109703

Regions

Domain276 – 35479PUA
Nucleotide binding170 – 1712ATP By similarity
Nucleotide binding212 – 2187ATP By similarity

Sites

Binding site111ATP By similarity
Binding site511Substrate By similarity
Binding site1381Substrate By similarity
Binding site1501Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7N7B2 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 74587DB033C1A6EA

FASTA36839,455
        10         20         30         40         50         60 
MMNSSQTLVV KLGTSVLTGG SRRLNRAHIV ELVRQCAQQH EKGHRIIIVT SGAIAAGREH 

        70         80         90        100        110        120 
LGYPDLPATI ASKQLLAAVG QSRLIQLWEQ FFSIYGIHVG QMLLTRADLE DRERFLNARD 

       130        140        150        160        170        180 
TLQALLDNRI VPVINENDAV ATAEIKVGDN DNLSALAAIL GGADKLLLLT DIEGLYTADP 

       190        200        210        220        230        240 
RNNPDAKLIP EVYDISDELR MVAGDSISGL GTGGMATKLQ AAGIAGRAGI DVIIAAGNKP 

       250        260        270        280        290        300 
DVISDVIEGN PVGTRFHGLE SPMENRKRWI FGAPPAGEII VDHGAETAIC EKGSSLLPKG 

       310        320        330        340        350        360 
IKNIKGDFSR GEVICIRSLS GKDLAHGVCR YNSDALRLIA GHHSQQISQI LGYEYGAVAV 


HRDDMIVS 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX571863 Genomic DNA. Translation: CAE13537.1.
RefSeqNP_928554.1. NC_005126.1.

3D structure databases

ProteinModelPortalQ7N7B2.
SMRQ7N7B2. Positions 5-366.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243265.plu1243.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE13537; CAE13537; plu1243.
GeneID2801207.
KEGGplu:plu1243.
PATRIC20506193. VBIPhoLum48522_1370.

Organism-specific databases

GenoListplu1243.
CMRSearch...

Phylogenomic databases

eggNOGCOG0263.
HOGENOMHOG000246369.
KOK00931.
OMAMRMIAGH.
OrthoDBEOG6PGK7G.

Enzyme and pathway databases

UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB_PHOLL
AccessionPrimary (citable) accession number: Q7N7B2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: December 15, 2003
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways