Q7N7A4 (GSH1_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--cysteine ligase
EC=6.3.2.2

Alternative name(s):
Gamma-ECS
Short name=GCS
Gamma-glutamylcysteine synthetase

Gene names

Name:	gshA
Ordered Locus Names:	plu1252

Organism

Photorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]

Taxonomic identifier

243265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP MF_00578
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP MF_00578
Sequence similarities	Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 519	519	Glutamate--cysteine ligase HAMAP MF_00578		PRO_0000192530

Sequences

	Sequence		Length	Mass (Da)
	Q7N7A4 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 4F478A73A07BB2C0	FASTA	519	58,738
	10 20 30 40 50 60 MIPDVSRALS WLEAHPTILK GIRRGVERET LRITSDGSLA ITEHPKTLGA ALTHKWITTD 70 80 90 100 110 120 FAESLLEFIT PAGDDIKYTI SFLSDIHRHT ARVLDKEKMW PLSMPCFIDA EENITLAQYG 130 140 150 160 170 180 ASNVGRYKTL YREGLKNRYG ALMQTISGVH YNFSLPIEFW RAWVGVEDAE SGKEQISNGY 190 200 210 220 230 240 FRLIRNYYRF GWVIPYLFGA SPAICSSFLR GRETSFLFER TEDGTCYLPY ATSLRMSNLG 250 260 270 280 290 300 YTNKSQSDLN ITFNNLHTYI DGLKKAIHKP SDEFAKLGTK QGDKHIQLNT NVLQIENELY 310 320 330 340 350 360 APIRPKRVTR GNESPSDALL RGGVEYIEVR SLDINPFAAI GVNETQIRFL DLFLIWCVLA 370 380 390 400 410 420 EAPEMGSDEL DCCRKNWNRV ILEGRKPGQM IGLGCGSVEE PLAKVGKKLF SDLKRVAAIL 430 440 450 460 470 480 DSCSGTQYQK VCEELIATFD DSSLTLSARV LEKMKSQGIG GFGLELADEY HQQLISEKYE 490 500 510 VISDEQFAIE RRASVERQDA LEREDTMSFD EYLKQQTGC

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571863 Genomic DNA. Translation: CAE13546.1.
RefSeq	NP_928563.1. NC_005126.1.
3D structure databases
ProteinModelPortal	Q7N7A4.
SMR	Q7N7A4. Positions 1-502.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2801220.
GenomeReviews	Gene locus plu1252 in contig BX470251_GR.
KEGG	plu:plu1252.
NMPDR	fig|243265.1.peg.1187.
PATRIC	20506225. VBIPhoLum48522_1382.
Organism-specific databases
GenoList	plu1252.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289023.
OMA	FGLIRNY.
PhylomeDB	Q7N7A4.
ProtClustDB	PRK02107.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU1252-MONOMER.
Family and domain databases
HAMAP	MF_00578. Glu_cys_ligase. [Tree]
InterPro	IPR007370. Glu_cys_ligase. IPR006334. Glut_cys_ligase. [Graphical view]
KO	K01919.
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01434. Glu_cys_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_PHOLL

Accession

Primary (citable) accession number: Q7N7A4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program