Reviewed, UniProtKB/Swiss-Prot Q7N743 (SYQ_PHOLL)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	plu1319

Organism

Photorhabdus luminescens subsp. laumondii [Complete proteome] [HAMAP]

Taxonomic identifier

141679 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00126
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_0000195842
Regions
	Motif	34 – 44	11	"HIGH" region HAMAP MF_00126
	Motif	268 – 272	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7N743-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: B13858B775EBCD58	FASTA	555	64,117
	10 20 30 40 50 60 MSEADARPTN FIRQIIDEDL ATGKHTSVHT RFPPEPNGYL HIGHAKSICL NFGVAKDYQG 70 80 90 100 110 120 QCNLRFDDTN PVKEDVEYVD SIKNDVQWLG FEWSGDIRYS SNYFDTLYQY AIELINKGLA 130 140 150 160 170 180 YVDQLSPEEI REYRGTLKEP GKNSPYRDRT IEENLVLFEK MRAGEFDEGK ACLRAKIDMA 190 200 210 220 230 240 SPFIVMRDPV LYRIKFAEHH QCGNKWCIYP MYDFTHCISD ALEGITHSLC TLEFQDNRRL 250 260 270 280 290 300 YDWVLENITI DCRPRQYEFS RLNLEYTVMS KRKLNLLVAD KIVEGWDDPR MPTISGLRRR 310 320 330 340 350 360 GYTAASIREF CRRIGVTKQD NNVEMAALES CIRDDLNENA PRAMAVLDPV RLVIENMPEG 370 380 390 400 410 420 EQILSMPNHP NKPEMGSREV PFSREIYIDR ADFREEANRQ YKRLVLGKEV RLRNAYIIKA 430 440 450 460 470 480 ERVEKDADDN ITTIYCSYDD QTLNKDPADG RKVKGVIHWV SAVHAVAAEF RLYDRLFCVP 490 500 510 520 530 540 NPGAEDDFLA TINPESLVIR QGFVEPSLAD AKSEQTYQFE REGYFCADSC HSSAENLVFN 550 RTAGLRDTWA KISQD

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571863 Genomic DNA. Translation: CAE13612.1.
RefSeq	NP_928629.1.
3D structure databases
SMR	Q7N743. Positions 9-548.
ModBase	Search...
Genome annotation databases
GeneID	2801297.
GenomeReviews	Gene locus plu1319 in contig BX470251_GR.
KEGG	plu:plu1319.
NMPDR	fig|243265.1.peg.1253.
Organism-specific databases
PhotoList	plu1319.
CMR	Search...
Phylogenomic databases
HOGENOM	Q7N743.
OMA	RMPTIAG.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU1319-MON.
BRENDA	6.1.1.18. 308689.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_PHOLL

Accession

Primary (citable) accession number: Q7N743

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	December 15, 2003
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)