Reviewed, UniProtKB/Swiss-Prot Q7N6K4 (MAO1_PHOLL)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	plu1546

Organism

Photorhabdus luminescens subsp. laumondii [Complete proteome] [HAMAP]

Taxonomic identifier

141679 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity. HAMAP MF_01619
Subunit structure	Homotetramer By similarity. HAMAP MF_01619
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	NAD-dependent malic enzyme HAMAP MF_01619		PRO_0000160220
Sites
	Active site	104	1	Proton donor By similarity
	Active site	175	1	Proton acceptor By similarity
	Metal binding	246	1	Divalent metal cation By similarity
	Metal binding	247	1	Divalent metal cation By similarity
	Metal binding	270	1	Divalent metal cation By similarity
	Binding site	157	1	NAD By similarity
	Binding site	270	1	NAD By similarity
	Binding site	418	1	NAD By similarity
	Site	270	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7N6K4-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: F15FE165CCAC9CC7	FASTA	565	63,000
	10 20 30 40 50 60 MELEHESKRP LYIPYSGPIL LEFPLLNKGS AFTEEERRNF NLHGLLPEAV ETIEEQAERA 70 80 90 100 110 120 YRQYLDFKND ADKHIYLRNI QDTNETLFYR LLDAHLNEMM PIIYTPTVGE ACEHFSDIYR 130 140 150 160 170 180 RARGLFISHP NKAHIDDMLQ NATKQNVKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT 190 200 210 220 230 240 SCGGISPAYT LPVVLDVGTN NPQRLNDPLY MGWRHPRITG KEYDEFVDEF IQAVKRRWPN 250 260 270 280 290 300 VLLQFEDFAQ KNAMPLLNRY RHEICCFNDD IQGTAAVTLG SLIAASRAAG RQLKDQTVTF 310 320 330 340 350 360 LGAGSAGCGI AEQIVAQMKS EGLSDEQARA RIFMVDRFGL LTDKLPNLLD FQNKLVQKSS 370 380 390 400 410 420 SLAKWDVNND SISLLDVVRN AKPTVLIGVS GQAGLFTEEI IREMHKHCER PIVMPLSNPT 430 440 450 460 470 480 SRVEARPEDI INWTDGAALV ATGSPFNPVK YKDQEYPIAQ CNNAYIFPGI GLGVIASGAK 490 500 510 520 530 540 LVTDGMLMAA SRTLANCSPL AQEGQGPLLP LIDDIQEVSR KIAKQVAKEA QIQGVATVTS 550 560 DGALDEAIER NFWKPEYRVY KRTSF

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571864 Genomic DNA. Translation: CAE13839.1.
RefSeq	NP_928837.1.
3D structure databases
SMR	Q7N6K4. Positions 16-565.
ModBase	Search...
Genome annotation databases
GeneID	2801541.
KEGG	plu:plu1546.
NMPDR	fig|243265.1.peg.1461.
Organism-specific databases
PhotoList	plu1546.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289821.
OMA	IQDNNET.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU1546-MONOMER.
BRENDA	1.1.1.38. 308689.
Family and domain databases
HAMAP	MF_01619. NAD_malic_enz. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_PHOLL

Accession

Primary (citable) accession number: Q7N6K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 15, 2003
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)