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Protein

Putative histidine biosynthesis bifunctional protein HisCD

Gene

hisCD

Organism
Photorhabdus luminescens subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.By similarity

Catalytic activityi

L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate.
L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: L-histidine biosynthesis

This protein is involved in step 7 and 9 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG)
  2. Histidine biosynthesis bifunctional protein HisIE (hisI)
  3. Histidine biosynthesis bifunctional protein HisIE (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase 1 (hisA1), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase 2 (hisA2)
  5. Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisH (hisH)
  6. Histidine biosynthesis bifunctional protein HisB (hisB)
  7. Putative histidine biosynthesis bifunctional protein HisCD (hisCD)
  8. Histidine biosynthesis bifunctional protein HisB (hisB)
  9. Putative histidine biosynthesis bifunctional protein HisCD (hisCD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi261ZincBy similarity1
Metal bindingi264ZincBy similarity1
Active sitei328By similarity1
Active sitei329By similarity1
Metal bindingi362ZincBy similarity1
Metal bindingi421ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Oxidoreductase, Transferase
Biological processAmino-acid biosynthesis, Histidine biosynthesis
LigandMetal-binding, NAD, Pyridoxal phosphate, Zinc

Enzyme and pathway databases

BioCyciPLUM243265:G1GTE-1617-MONOMER
UniPathwayiUPA00031; UER00012
UPA00031; UER00014

Names & Taxonomyi

Protein namesi
Recommended name:
Putative histidine biosynthesis bifunctional protein HisCD
Including the following 2 domains:
Histidinol dehydrogenase (EC:1.1.1.23)
Short name:
HDH
Histidinol-phosphate aminotransferase (EC:2.6.1.9)
Alternative name(s):
Imidazole acetol-phosphate transaminase
Gene namesi
Name:hisCD
Synonyms:hisD
Ordered Locus Names:plu1569
OrganismiPhotorhabdus luminescens subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)
Taxonomic identifieri243265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesMorganellaceaePhotorhabdus
Proteomesi
  • UP000002514 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001534151 – 807Putative histidine biosynthesis bifunctional protein HisCDAdd BLAST807

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei655N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PRIDEiQ7N6I1

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi243265.plu1569

Structurei

3D structure databases

ProteinModelPortaliQ7N6I1
SMRiQ7N6I1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 440Histidinol dehydrogenaseAdd BLAST440
Regioni441 – 807Histidinol-phosphate aminotransferaseAdd BLAST367

Sequence similaritiesi

In the N-terminal section; belongs to the histidinol dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4105CEK Bacteria
COG0079 LUCA
COG0141 LUCA
KOiK00013
OrthoDBiPOG091H03YX

Family and domain databases

CDDicd06572 Histidinol_dh, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
HAMAPiMF_01023 HisC_aminotrans_2, 1 hit
MF_01024 HisD, 1 hit
InterProiView protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR001917 Aminotrans_II_pyridoxalP_BS
IPR004839 Aminotransferase_I/II
IPR005861 HisP_aminotrans
IPR016298 Histidine_synth_trifunct
IPR001692 Histidinol_DH_CS
IPR012131 Hstdl_DH
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR21256 PTHR21256, 1 hit
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
PF00815 Histidinol_dh, 1 hit
PIRSFiPIRSF001257 His_trifunctional, 1 hit
PRINTSiPR00083 HOLDHDRGNASE
SUPFAMiSSF53383 SSF53383, 1 hit
SSF53720 SSF53720, 1 hit
TIGRFAMsiTIGR01141 hisC, 1 hit
TIGR00069 hisD, 1 hit
PROSITEiView protein in PROSITE
PS00599 AA_TRANSFER_CLASS_2, 1 hit
PS00611 HISOL_DEHYDROGENASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q7N6I1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDHFDTLIR WQECHDEQQN ALLTRPAISA SVEISRTVEQ ILHAVKEYGD
60 70 80 90 100
HTLREFSRRF DKTVIENIRI SPEEIAAAEN SLNNDIKQAM QQAMNNIRVF
110 120 130 140 150
HEAQKPIKIE VETQPGVYCQ QVTRPIDSVG LYIPGGSAPL LSTVLMLGTP
160 170 180 190 200
AQIAGCHKVV LCSPPPIANE ILYAATLCGI TEIFQIGGAQ AIAAMAFGTE
210 220 230 240 250
SVPKVDKIFG PGNAYVTEAK RQVSQRVDGA TIDMPAGPSE LLIIADAGAN
260 270 280 290 300
PVFVAADLLS QAEHGPDSQV ILVTPDEALA KKVITEIEKQ LTRLPRNQIA
310 320 330 340 350
AKALAHSRII VTTSLQQCVE ISNRYGPEHL IIQTRQPEQL VEKITSAGSV
360 370 380 390 400
FLGDWSPESA GDYASGTNHV LPTYGYTSTY SSLGLADFLK RMTIQQLTPQ
410 420 430 440 450
GLLNLSQTIE TLAQAEQLTA HKNALTLRVA ALNIAGQGVN MNNIFDANLL
460 470 480 490 500
ARENIRKLTP YMSARRLGGK GDVWLNANEY PLAPDFQCTE QTLNRYPDCQ
510 520 530 540 550
PASVIRRYAA YAGLQPEQVL ACRGADESIE LLIRVFCEPG QDVVLFCPPT
560 570 580 590 600
YGMYSVSAET FGVEQKKITA LENWQLDIEA IENNLDRVKL IYICSPNNPT
610 620 630 640 650
GNAINPDSLR KILELTANRA IVTIDEAYIE FCPENSIASW LKNYPNLVIL
660 670 680 690 700
RTLSKAFALA GLRCGFTLAS VDIITLLLKV IAPYPLSTPV ADIAAQALTA
710 720 730 740 750
ENIAIMQKRV VEIRENRNDL QQALNKLAIV EKVFPSETNY ILVKFYDAET
760 770 780 790 800
VFKTLWHQGI ILRDQRKQPG LEGCLRITIG SRKECERVVE AISALSTVNE

QPKEIAN
Length:807
Mass (Da):88,850
Last modified:December 15, 2003 - v1
Checksum:i648F98DD2F9F706E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571864 Genomic DNA Translation: CAE13862.1
RefSeqiWP_011145866.1, NC_005126.1

Genome annotation databases

EnsemblBacteriaiCAE13862; CAE13862; plu1569
KEGGiplu:plu1569

Similar proteinsi

Entry informationi

Entry nameiHIS8_PHOLL
AccessioniPrimary (citable) accession number: Q7N6I1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 15, 2003
Last modified: April 25, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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