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Protein

Putative histidine biosynthesis bifunctional protein HisCD

Gene

hisCD

Organism
Photorhabdus luminescens subsp. laumondii (strain TT01)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.By similarity

Catalytic activityi

L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate.
L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi261 – 2611ZincBy similarity
Metal bindingi264 – 2641ZincBy similarity
Active sitei328 – 3281By similarity
Active sitei329 – 3291By similarity
Metal bindingi362 – 3621ZincBy similarity
Metal bindingi421 – 4211ZincBy similarity

GO - Molecular functioni

  1. histidinol dehydrogenase activity Source: UniProtKB-EC
  2. histidinol-phosphate transaminase activity Source: UniProtKB-EC
  3. L-phenylalanine:2-oxoglutarate aminotransferase activity Source: UniProtKB-EC
  4. NAD binding Source: InterPro
  5. pyridoxal phosphate binding Source: InterPro
  6. zinc ion binding Source: InterPro

GO - Biological processi

  1. histidine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Pyridoxal phosphate, Zinc

Enzyme and pathway databases

UniPathwayiUPA00031; UER00012.
UPA00031; UER00014.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative histidine biosynthesis bifunctional protein HisCD
Including the following 2 domains:
Histidinol dehydrogenase (EC:1.1.1.23)
Short name:
HDH
Histidinol-phosphate aminotransferase (EC:2.6.1.9)
Alternative name(s):
Imidazole acetol-phosphate transaminase
Gene namesi
Name:hisCD
Synonyms:hisD
Ordered Locus Names:plu1569
OrganismiPhotorhabdus luminescens subsp. laumondii (strain TT01)
Taxonomic identifieri243265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePhotorhabdus
ProteomesiUP000002514 Componenti: Chromosome

Organism-specific databases

GenoListiplu1569.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 807807Putative histidine biosynthesis bifunctional protein HisCDPRO_0000153415Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei655 – 6551N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PRIDEiQ7N6I1.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi243265.plu1569.

Structurei

3D structure databases

ProteinModelPortaliQ7N6I1.
SMRiQ7N6I1. Positions 5-432, 450-794.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 440440Histidinol dehydrogenaseAdd
BLAST
Regioni441 – 807367Histidinol-phosphate aminotransferaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the histidinol dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG0079.
KOiK00013.
OMAiNTIAREM.
OrthoDBiEOG6CVVCR.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01023. HisC_aminotrans_2.
MF_01024. HisD.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR005861. HisP_aminotrans.
IPR001692. Histidinol_DH_CS.
IPR012131. Hstdl_DH.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF00815. Histidinol_dh. 1 hit.
[Graphical view]
PRINTSiPR00083. HOLDHDRGNASE.
SUPFAMiSSF53383. SSF53383. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01141. hisC. 1 hit.
TIGR00069. hisD. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
PS00611. HISOL_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7N6I1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDHFDTLIR WQECHDEQQN ALLTRPAISA SVEISRTVEQ ILHAVKEYGD
60 70 80 90 100
HTLREFSRRF DKTVIENIRI SPEEIAAAEN SLNNDIKQAM QQAMNNIRVF
110 120 130 140 150
HEAQKPIKIE VETQPGVYCQ QVTRPIDSVG LYIPGGSAPL LSTVLMLGTP
160 170 180 190 200
AQIAGCHKVV LCSPPPIANE ILYAATLCGI TEIFQIGGAQ AIAAMAFGTE
210 220 230 240 250
SVPKVDKIFG PGNAYVTEAK RQVSQRVDGA TIDMPAGPSE LLIIADAGAN
260 270 280 290 300
PVFVAADLLS QAEHGPDSQV ILVTPDEALA KKVITEIEKQ LTRLPRNQIA
310 320 330 340 350
AKALAHSRII VTTSLQQCVE ISNRYGPEHL IIQTRQPEQL VEKITSAGSV
360 370 380 390 400
FLGDWSPESA GDYASGTNHV LPTYGYTSTY SSLGLADFLK RMTIQQLTPQ
410 420 430 440 450
GLLNLSQTIE TLAQAEQLTA HKNALTLRVA ALNIAGQGVN MNNIFDANLL
460 470 480 490 500
ARENIRKLTP YMSARRLGGK GDVWLNANEY PLAPDFQCTE QTLNRYPDCQ
510 520 530 540 550
PASVIRRYAA YAGLQPEQVL ACRGADESIE LLIRVFCEPG QDVVLFCPPT
560 570 580 590 600
YGMYSVSAET FGVEQKKITA LENWQLDIEA IENNLDRVKL IYICSPNNPT
610 620 630 640 650
GNAINPDSLR KILELTANRA IVTIDEAYIE FCPENSIASW LKNYPNLVIL
660 670 680 690 700
RTLSKAFALA GLRCGFTLAS VDIITLLLKV IAPYPLSTPV ADIAAQALTA
710 720 730 740 750
ENIAIMQKRV VEIRENRNDL QQALNKLAIV EKVFPSETNY ILVKFYDAET
760 770 780 790 800
VFKTLWHQGI ILRDQRKQPG LEGCLRITIG SRKECERVVE AISALSTVNE

QPKEIAN
Length:807
Mass (Da):88,850
Last modified:December 14, 2003 - v1
Checksum:i648F98DD2F9F706E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571864 Genomic DNA. Translation: CAE13862.1.
RefSeqiNP_928860.1. NC_005126.1.

Genome annotation databases

KEGGiplu:plu1569.
PATRICi20507017. VBIPhoLum48522_1751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571864 Genomic DNA. Translation: CAE13862.1.
RefSeqiNP_928860.1. NC_005126.1.

3D structure databases

ProteinModelPortaliQ7N6I1.
SMRiQ7N6I1. Positions 5-432, 450-794.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243265.plu1569.

Proteomic databases

PRIDEiQ7N6I1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiplu:plu1569.
PATRICi20507017. VBIPhoLum48522_1751.

Organism-specific databases

GenoListiplu1569.

Phylogenomic databases

eggNOGiCOG0079.
KOiK00013.
OMAiNTIAREM.
OrthoDBiEOG6CVVCR.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00012.
UPA00031; UER00014.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01023. HisC_aminotrans_2.
MF_01024. HisD.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR005861. HisP_aminotrans.
IPR001692. Histidinol_DH_CS.
IPR012131. Hstdl_DH.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF00815. Histidinol_dh. 1 hit.
[Graphical view]
PRINTSiPR00083. HOLDHDRGNASE.
SUPFAMiSSF53383. SSF53383. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01141. hisC. 1 hit.
TIGR00069. hisD. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
PS00611. HISOL_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TT01.

Entry informationi

Entry nameiHIS8_PHOLL
AccessioniPrimary (citable) accession number: Q7N6I1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 18, 2004
Last sequence update: December 14, 2003
Last modified: March 31, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.