Reviewed, UniProtKB/Swiss-Prot Q7N574 (LUXB_PHOLL)

Names and origin

Protein names

Recommended name:
    Alkanal monooxygenase beta chain
    EC=1.14.14.3
Alternative name(s):
    Bacterial luciferase beta chain

Gene names

Name:	luxB
Ordered Locus Names:	plu2082

Organism

Photorhabdus luminescens subsp. laumondii [Complete proteome] [HAMAP]

Taxonomic identifier

141679 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity By similarity.
Catalytic activity	RCHO + reduced FMN + O(2) = RCOOH + FMN + H(2)O + light.
Subunit structure	Heterodimer of an alpha and a beta chain By similarity.
Sequence similarities	Belongs to the bacterial luciferase oxidoreductase family.

Ontologies

Keywords
Biological process	Luminescence
Ligand	FMN Flavoprotein
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkanal monooxygenase (FMN-linked) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Alkanal monooxygenase beta chain		PRO_0000220176

Sequences

	Sequence		Length	Mass (Da)
	Q7N574-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: A5EA280507019967	FASTA	324	37,291
	10 20 30 40 50 60 MKFGLFFLNF INSTTVQEQS IVRMQEITEY VDKLNFEQIL VYENHFSGNG VVGAPLTVSG 70 80 90 100 110 120 FLLGLTEKIK IGSLNHIITT HHPVRIAEEA CLLDQLSEGR FILGFSDCEK KDEMRLFNRP 130 140 150 160 170 180 VEYQQQLFEE CYEIINDALT TGYCNPDNDF YSFPKISVNP HAYTQGGPRR YVTATSHHIV 190 200 210 220 230 240 EWAAKKGIPL IFKWDDSNDV RYEYAERYKA VADKYGIDLS AIDHQLMVLV NYNEDSHKAK 250 260 270 280 290 300 QETRAFIRDY VLEMYPNENL ENKLEEIITE NAVGDYTECI AAAKLAIEKC GAKSVLLSFE 310 320 PMNDLMHQKN VINIVNDNIK KYHM

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571866 Genomic DNA. Translation: CAE14375.1.
RefSeq	NP_929343.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2802086.
GenomeReviews	Gene locus plu2082 in contig BX470251_GR.
KEGG	plu:plu2082.
NMPDR	fig|243265.1.peg.1967.
Organism-specific databases
PhotoList	plu2082.
CMR	Search...
Phylogenomic databases
HOGENOM	Q7N574.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU2082-MON.
Family and domain databases
InterPro	IPR011251. Luciferase-like_bac. IPR002103. Luciferase_bac. IPR016048. Luciferase_mOase. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
PRINTS	PR00089. LUCIFERASE.
PROSITE	PS00494. BACTERIAL_LUCIFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXB_PHOLL

Accession

Primary (citable) accession number: Q7N574

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	December 15, 2003
Last modified:	November 25, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)