Reviewed, UniProtKB/Swiss-Prot Q7N571 (GHRA_PHOLL)

Names and origin

Protein names

Recommended name:
    Glyoxylate/hydroxypyruvate reductase A
    EC=1.1.1.79
    EC=1.1.1.81
Alternative name(s):
    2-ketoacid reductase

Gene names

Name:	ghrA
Ordered Locus Names:	plu2086

Organism

Photorhabdus luminescens subsp. laumondii [Complete proteome] [HAMAP]

Taxonomic identifier

141679 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity. HAMAP MF_01666
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01666 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01666
Subcellular location	Cytoplasm Probable HAMAP MF_01666.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	cofactor binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Glyoxylate/hydroxypyruvate reductase A HAMAP MF_01666		PRO_0000348366
Sites
	Active site	228	1	By similarity
	Active site	276	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7N571-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: D2468F3F3AC11161	FASTA	313	35,508
	10 20 30 40 50 60 MEIIFYHPFF DAAQWIQGMQ QRLPNINIRQ WKRGDNKHAD YAMVWAPPYE MLANRSGLKG 70 80 90 100 110 120 IFILGAGVEA ILKQEQQKPG MLPAGVPLMR LEDAGMGLQM QEYAVAMVLH YLRRMDEYKL 130 140 150 160 170 180 QQGQRRWKQL EPYDRKDFVV GVLGAGVLGR RVAQTLVEWG FIVRCWSRTP KQINNVVSFH 190 200 210 220 230 240 GEDQLGDFLS GSKVLINLLP DTPKTRGILN LSLFSQLKPK SYLINIARGA HLVEHDLLVA 250 260 270 280 290 300 IDKGYIVGAS LDVFVEEPLP EMHPFWTHPR ITVTPHVAAI TIPDIAMDTI SENIRRIEKG 310 ELSTGVVDIK LGY

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571866 Genomic DNA. Translation: CAE14379.1.
RefSeq	NP_929347.1.
3D structure databases
SMR	Q7N571. Positions 1-300.
ModBase	Search...
Genome annotation databases
GeneID	2802091.
KEGG	plu:plu2086.
NMPDR	fig|243265.1.peg.1971.
Organism-specific databases
PhotoList	plu2086.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG731446.
OMA	YALVWRA.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU2086-MONOMER.
Family and domain databases
HAMAP	MF_01666. 2-Hacid_dh_C_GhrA. [Tree]
InterPro	IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRA_PHOLL

Accession

Primary (citable) accession number: Q7N571

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 15, 2003
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)