Q7N4V9 (HCAF_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phenylpropionate/cinnamic acid dioxygenase subunit beta
EC=1.14.12.19

Alternative name(s):
Digoxigenin subunit beta

Gene names

Name:	hcaF
Ordered Locus Names:	plu2205

Organism

Photorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]

Taxonomic identifier

243265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively By similarity. HAMAP MF_01649
Catalytic activity	3-phenylpropanoate + NADH + O2 = 3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+. (2E)-3-phenylprop-2-enoate + NADH + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NAD+.
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01649
Subunit structure	This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (hcaE and hcaF), a ferredoxin (hcaC) and a ferredoxin reductase (hcaD) By similarity.
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-phenylpropionate dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 180	180	3-phenylpropionate/cinnamic acid dioxygenase subunit beta HAMAP MF_01649		PRO_0000333713

Sequences

	Sequence		Length	Mass (Da)
	Q7N4V9 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 3F29E545C70C1065	FASTA	180	21,783
	10 20 30 40 50 60 MKELIVNQEK RVSPELHYEI SQFLYYEIAL LDEWRFRDWL ELLSEDLSYT MRTIVNAQTR 70 80 90 100 110 120 DRRKSIQPPT TWLFNDNKFQ LERRIARLET GMAWAEEPPS RTRHLLTNLV ITETDLPDQF 130 140 150 160 170 180 HVQSNYLLYR SQKERDEVFY VGKRLDCVRR NPKTDSWLIC KREITLDQAV LTFHNLSVLF

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571866 Genomic DNA. Translation: CAE14498.1.
RefSeq	NP_929463.1. NC_005126.1.
3D structure databases
HSSP	HSSP built from PDB template 2B1X based on UniProtKB Q9WVZ0.
ProteinModelPortal	Q7N4V9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2802210.
GenomeReviews	Gene locus plu2205 in contig BX470251_GR.
KEGG	plu:plu2205.
NMPDR	fig|243265.1.peg.2087.
PATRIC	20508521. VBIPhoLum48522_2499.
Organism-specific databases
GenoList	plu2205.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG582498.
OMA	ARRTICV.
ProtClustDB	PRK10069.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU2205-MONOMER.
Family and domain databases
HAMAP	MF_01649. HcaF. [Tree]
InterPro	IPR023712. HcaF. IPR000391. Rng_hydr_dOase-bsu. [Graphical view]
KO	K05709.
Pfam	PF00866. Ring_hydroxyl_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HCAF_PHOLL

Accession

Primary (citable) accession number: Q7N4V9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program