Q7N4V6 (MHPB_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
EC=1.13.11.16

Gene names

Name:	mhpB
Ordered Locus Names:	plu2208

Organism

Photorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]

Taxonomic identifier

243265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity. HAMAP MF_01653
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653 (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653
Cofactor	Fe2+ ion By similarity. HAMAP MF_01653
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01653
Subunit structure	Homotetramer By similarity. HAMAP MF_01653
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase HAMAP MF_01653		PRO_0000337661
Sites
	Active site	115	1	Proton donor By similarity
	Active site	179	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7N4V6 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 900635327D4E8BC6	FASTA	317	34,977
	10 20 30 40 50 60 MTVKLICTSH TPLMGFGSPP EATEKHVRQV FQQLAEQIKD YDPQLIVIFA PDHFNGFFYD 70 80 90 100 110 120 LMPAFCVGVR ANAVGDWDIG KGPLNVPENT AKDLISALYD SGIDVAHSWR MQVDHGFTQP 130 140 150 160 170 180 LMLLCQNLQR YPTIPIFINC AAKPLPTCRR AVELGRAVGQ FLFTTDQRVL LLGSGGLSHD 190 200 210 220 230 240 PPIPQMGQVP PEVEEGLIAG RNPTKEARQK RQIRVIEVGK SLARGENIVA PLNPQWDDEL 250 260 270 280 290 300 LRIFCSGDIR RLASLTEGGI AIQGGKGGQE IRCWIAAFAA LSVYGEYKAQ RHYYQPIKEW 310 LAGMAMVSAQ PVTQIGA

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571866 Genomic DNA. Translation: CAE14501.1.
RefSeq	NP_929466.1. NC_005126.1.
3D structure databases
ProteinModelPortal	Q7N4V6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2802213.
GenomeReviews	Gene locus plu2208 in contig BX470251_GR.
KEGG	plu:plu2208.
NMPDR	fig|243265.1.peg.2090.
PATRIC	20508527. VBIPhoLum48522_2502.
Organism-specific databases
GenoList	plu2208.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG565586.
OMA	AHEIRTW.
ProtClustDB	PRK13370.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU2208-MONOMER.
Family and domain databases
HAMAP	MF_01653. MhpB. [Tree]
InterPro	IPR023789. DHPP/DHXA_dioxygenase. IPR004183. Xdiol_dOase_suB. [Graphical view]
Gene3D	G3DSA:3.40.830.10. Xdiol_dOase_3B. 2 hits.
KO	K05713.
Pfam	PF02900. LigB. 1 hit. [Graphical view]
SUPFAM	SSF53213. Xdiol_dOase_3B. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MHPB_PHOLL

Accession

Primary (citable) accession number: Q7N4V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program