Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q7N4V5 (HCAD_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component
EC=1.18.1.3
Alternative name(s):
Digoxigenin system ferredoxin--NAD(+) reductase component
Gene names
Name:hcaD
Ordered Locus Names:plu2209
OrganismPhotorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]
Taxonomic identifier243265 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Part of the multicomponent 3-phenylpropionate dioxygenase, that converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively By similarity. HAMAP MF_01651

Catalytic activity

Reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH. HAMAP MF_01651

Cofactor

FAD By similarity. HAMAP MF_01651

Pathway

Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01651

Subunit structure

This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (hcaE and hcaF), a ferredoxin (hcaC) and a ferredoxin reductase (hcaD) By similarity.

Sequence similarities

Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3943943-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component HAMAP MF_01651
PRO_0000333727

Regions

Nucleotide binding5 – 3632FAD Potential
Nucleotide binding146 – 17429NAD Potential

Sequences

Sequence LengthMass (Da)Tools
Q7N4V5 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: F5977C83A10EBBC9

FASTA39443,579
        10         20         30         40         50         60 
MRNQTFIIVG AGQAGAMAAA TLRQQQFDGD IILIGKEYHA PYERPILSKD YLINPEEAPK 

        70         80         90        100        110        120 
YLFSEDFYLE KQIDLRIGQL VSQIMPSKHC VVLENGGKLR YDKLLLTMGA RARRFPLLDQ 

       130        140        150        160        170        180 
LGENIYTLRT LDDAQRLRQA VKKDKRILIV GGGVIGLELA ATSCELGANV TVIEQADNIM 

       190        200        210        220        230        240 
GRCAPPLLQD YLLNRHQEKG VQFFLDTNIV SAQKQGSELV LILNTGEKVI GDIIIYGIGA 

       250        260        270        280        290        300 
EFRDQLAADA GLVTDGGIVI DSRCQTSEPD IFAAGDVCLQ REPLTGDLQR RETWENANRQ 

       310        320        330        340        350        360 
ATIAAHAMMG LAPPQPGAPW FWTDQWGINI QMVGNMQAEE WHIQGDLQSD KAILFGTENE 

       370        380        390 
VLVGAVAINQ GREMRNLRKL LANPAQVVSG VEWA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX571866 Genomic DNA. Translation: CAE14502.1.
RefSeqNP_929467.1. NC_005126.1.

3D structure databases

HSSPHSSP built from PDB template 1Q1R based on UniProtKB P16640.
ProteinModelPortalQ7N4V5.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2802214.
GenomeReviewsGene locus plu2209 in contig BX470251_GR.
KEGGplu:plu2209.
NMPDRfig|243265.1.peg.2091.
PATRIC20508529. VBIPhoLum48522_2503.

Organism-specific databases

GenoListplu2209.
CMRSearch...

Phylogenomic databases

HOGENOMHBG644911.
OMANAQEQGT.
ProtClustDBPRK09754.

Enzyme and pathway databases

BioCycPLUM243265:PLU2209-MONOMER.

Family and domain databases

HAMAPMF_01651. HcaD.
[Tree]
InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR023744. HcaD.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
Gene3DG3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
KOK00529.
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHCAD_PHOLL
AccessionPrimary (citable) accession number: Q7N4V5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 15, 2003
Last modified: January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents