Reviewed, UniProtKB/Swiss-Prot Q7N4H8 (FUMC_PHOLL)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	plu2359

Organism

Photorhabdus luminescens subsp. laumondii [Complete proteome] [HAMAP]

Taxonomic identifier

141679 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161295
Regions
	Region	129 – 132	4	B site By similarity
	Region	139 – 141	3	Substrate binding By similarity
Sites
	Binding site	100	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7N4H8-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 085AB3882444AB3E	FASTA	464	50,119
	10 20 30 40 50 60 MAATRIEKDS MGPIEVPADQ LWGAQTQRSL EHFRISQEKM PVALIHALAL TKQAAASVNM 70 80 90 100 110 120 DLGLLPQERG EAIIAAAKEV LEGKHPTEFP LAIWQTGSGT QSNMNMNEVL ANRGSEILGG 130 140 150 160 170 180 VRGSERLIHP NDDVNKSQSS NDVFPTAMHV AAVIALSEHL IPELKILQKT LADKAEAYKD 190 200 210 220 230 240 IVKIGRTHLQ DATPLTLGQE ISGWAAMLTH NLKHIEDSIP HVCELALGGT AVGTGLNTHP 250 260 270 280 290 300 EYAVRVAKKL AELTNHSFVT APNKFEALAT CDALVHSHGA LKGLAASIMK IANDVRWLAS 310 320 330 340 350 360 GPRCGIGEIS IPENEPGSSI MPGKVNPTQC EAVTMLCAQV MGNDVAINIG GASGNFELNV 370 380 390 400 410 420 FRPMVINNFL QSVRLLADGM RSFNEHCAVG IEPNRDRITQ LLNESLMLVT ALNTHIGYDK 430 440 450 460 AAEIAKKAHK EGLTLKQSAM KLGYLTEAEF DEWVRPEDMV GSLK

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571866 Genomic DNA. Translation: CAE14652.1.
RefSeq	NP_929605.1.
3D structure databases
SMR	Q7N4H8. Positions 5-459.
ModBase	Search...
Genome annotation databases
GeneID	2802364.
GenomeReviews	Gene locus plu2359 in contig BX470251_GR.
KEGG	plu:plu2359.
NMPDR	fig|243265.1.peg.2229.
Organism-specific databases
PhotoList	plu2359.
CMR	Search...
Phylogenomic databases
HOGENOM	Q7N4H8.
OMA	GSQGHFE.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU2359-MON.
BRENDA	4.2.1.2. 308689.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_PHOLL

Accession

Primary (citable) accession number: Q7N4H8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)