Q7N3W6 (SYY1_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase 1
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase 1
Short name=TyrRS 1

Gene names

Name:	tyrS1
Ordered Locus Names:	plu2596

Organism

Photorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]

Taxonomic identifier

243265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02006
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity. HAMAP MF_02006
Subcellular location	Cytoplasm By similarity HAMAP MF_02006.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	Tyrosine--tRNA ligase 1 HAMAP MF_02006		PRO_0000234749
Regions
	Domain	357 – 414	58	S4 RNA-binding
	Motif	42 – 51	10	"HIGH" region HAMAP MF_02006
	Motif	235 – 239	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	37	1	Tyrosine By similarity
	Binding site	175	1	Tyrosine By similarity
	Binding site	179	1	Tyrosine By similarity
	Binding site	238	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7N3W6 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: B39BA872536DD41C	FASTA	424	47,211
	10 20 30 40 50 60 MSSNNLIKQL QERGLIAQVT DENALAERLA QGPVSLYCGF DPTADSLHLG HLVPLLCLKR 70 80 90 100 110 120 FQLAGHKPVA LVGGATGLIG DPSFKATERK LNTEATVKEW VEKIRRQVSP FLDFDCGENS 130 140 150 160 170 180 AKTANNYDWF GNMDVLAFLR DIGKHFSVNQ MINKEAVKQR LNRDDVGISF TEFSYNLLQS 190 200 210 220 230 240 YDFARLNEVH GVELQIGGSD QWGNITSGID LTRRITQKQV FGMTVPLITK SDGTKFGKTE 250 260 270 280 290 300 GGAVWLDPKK TSPYKFYQFW INTADADVYR FLKFFTFMNL EDIDALEEED KNSGKAPRAQ 310 320 330 340 350 360 YVLAEQVTGM VHGEEGLAAA KRITESLFSG AAADLTEADF AQLAQDGMPV IELEKGADLQ 370 380 390 400 410 420 QALVNAGLVP SRGQARTMIS SNAVAINGEK QSEPEYLFTD SNRLFDRYTL LRRGKKHDCL ICWK

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571867 Genomic DNA. Translation: CAE14970.1.
RefSeq	NP_929831.1. NC_005126.1.
3D structure databases
ProteinModelPortal	Q7N3W6.
SMR	Q7N3W6. Positions 1-322.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2802602.
GenomeReviews	Gene locus plu2596 in contig BX470251_GR.
KEGG	plu:plu2596.
NMPDR	fig|243265.1.peg.2455.
PATRIC	20509393. VBIPhoLum48522_2935.
Organism-specific databases
GenoList	plu2596.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288125.
OMA	TFYIGFD.
PhylomeDB	Q7N3W6.
ProtClustDB	PRK05912.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU2596-MONOMER.
Family and domain databases
HAMAP	MF_02006. Tyr_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. IPR024107. Tyr-tRNA-synth_bac_1. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY1_PHOLL

Accession

Primary (citable) accession number: Q7N3W6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program