Q7N2K3 (MEND_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
Short name=SEPHCHC synthase
EC=2.2.1.9

Alternative name(s):
Menaquinone biosynthesis protein MenD

Gene names

Name:	menD
Ordered Locus Names:	plu3073

Organism

Photorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]

Taxonomic identifier

243265 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus › Photorhabdus luminescens › Photorhabdus luminescens subsp. laumondii

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity. HAMAP-Rule MF_01659
Catalytic activity	Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2. HAMAP-Rule MF_01659
Cofactor	Magnesium or manganese By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 2/8. HAMAP-Rule MF_01659
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the TPP enzyme family. MenD subfamily.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis
Ligand	Magnesium Manganese Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	menaquinone biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP thiamine pyrophosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase HAMAP-Rule MF_01659		PRO_0000341796

Sequences

	Sequence		Length	Mass (Da)
	Q7N2K3 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 114D626C10D4EDAE	FASTA	564	62,123
	10 20 30 40 50 60 MSNSAFNRRW AELLLEALTR HGLRHVCIAP GSRSTPLTIA AAANNKLICH THFDERGLGH 70 80 90 100 110 120 LALGLSKASR EPVAVIVTSG TAVANLYPSL IEASLTGERM VFLTADRPPE LMDCGANQAI 130 140 150 160 170 180 RQNGIFASHP CQTISLPRPT VDIPASWLAS VIDHSMANLQ HGAVHINCPF AEPLYGNEEP 190 200 210 220 230 240 AYDEWQQQLG DWWKSKTPWL SQPVAKVVAE VPDWDCWQQK KGVVLAGRMS AEEGVNVAKW 250 260 270 280 290 300 AKALSWPLIG NALSQTGQPL PCADLWLDNP HVQEILAQAQ LVVQFGSSLI GKCLLQWQAK 310 320 330 340 350 360 CQPQEFWIID PIPGRLDPAN HRGKKLTCHI ADWLLAHPAQ PCIPWAQSCT LWAEELIVWS 370 380 390 400 410 420 EKAVNCVRTE LAGKFGEATV AYRLRELLPE RGQLFVGNSL IIRLIDTLGQ LPAGYPVYSN 430 440 450 460 470 480 RGASGIDGLI STAAGVQRAT AKPTLAVIGD LSALYDLNSL ALLRHPSAPM VLIVVNNNGG 490 500 510 520 530 540 QIFSLLPTQE EARQRFYCMP QYVNFDHAAA MFGLKYASPQ NWMELQQSIK QAWQQNETTL 550 560 IELKVPDREG AECLQQLLKQ VAML

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571869 Genomic DNA. Translation: CAE15447.1.
RefSeq	NP_930305.1. NC_005126.1.
3D structure databases
ProteinModelPortal	Q7N2K3.
ModBase	Search...
Protein-protein interaction databases
STRING	243265.plu3073.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE15447; CAE15447; plu3073.
GeneID	2803084.
KEGG	plu:plu3073.
PATRIC	20510496. VBIPhoLum48522_3487.
Organism-specific databases
GenoList	plu3073.
CMR	Search...
Phylogenomic databases
eggNOG	COG1165.
HOGENOM	HOG000218360.
KO	K02551.
OMA	DGGGIFH.
ProtClustDB	PRK07449.
Enzyme and pathway databases
UniPathway	UPA00079; UER00164.
Family and domain databases
HAMAP	MF_01659. MenD.
InterPro	IPR004433. MenaQ_synth_MenD. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR011766. TPP_enzyme-bd_C. [Graphical view]
PANTHER	PTHR18968:SF3. PTHR18968:SF3. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PIRSF	PIRSF004983. MenD. 1 hit.
TIGRFAMs	TIGR00173. menD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MEND_PHOLL

Accession

Primary (citable) accession number: Q7N2K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program