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Q7N2K3 (MEND_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Short name=SEPHCHC synthase
EC=2.2.1.9
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene names
Name:menD
Ordered Locus Names:plu3073
OrganismPhotorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]
Taxonomic identifier243265 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePhotorhabdus

Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity. HAMAP-Rule MF_01659

Catalytic activity

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2. HAMAP-Rule MF_01659

Cofactor

Magnesium or manganese By similarity. HAMAP-Rule MF_01659

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 2/8. HAMAP-Rule MF_01659

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01659

Sequence similarities

Belongs to the TPP enzyme family. MenD subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5645642-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase HAMAP-Rule MF_01659
PRO_0000341796

Sequences

Sequence LengthMass (Da)Tools
Q7N2K3 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 114D626C10D4EDAE

FASTA56462,123
        10         20         30         40         50         60 
MSNSAFNRRW AELLLEALTR HGLRHVCIAP GSRSTPLTIA AAANNKLICH THFDERGLGH 

        70         80         90        100        110        120 
LALGLSKASR EPVAVIVTSG TAVANLYPSL IEASLTGERM VFLTADRPPE LMDCGANQAI 

       130        140        150        160        170        180 
RQNGIFASHP CQTISLPRPT VDIPASWLAS VIDHSMANLQ HGAVHINCPF AEPLYGNEEP 

       190        200        210        220        230        240 
AYDEWQQQLG DWWKSKTPWL SQPVAKVVAE VPDWDCWQQK KGVVLAGRMS AEEGVNVAKW 

       250        260        270        280        290        300 
AKALSWPLIG NALSQTGQPL PCADLWLDNP HVQEILAQAQ LVVQFGSSLI GKCLLQWQAK 

       310        320        330        340        350        360 
CQPQEFWIID PIPGRLDPAN HRGKKLTCHI ADWLLAHPAQ PCIPWAQSCT LWAEELIVWS 

       370        380        390        400        410        420 
EKAVNCVRTE LAGKFGEATV AYRLRELLPE RGQLFVGNSL IIRLIDTLGQ LPAGYPVYSN 

       430        440        450        460        470        480 
RGASGIDGLI STAAGVQRAT AKPTLAVIGD LSALYDLNSL ALLRHPSAPM VLIVVNNNGG 

       490        500        510        520        530        540 
QIFSLLPTQE EARQRFYCMP QYVNFDHAAA MFGLKYASPQ NWMELQQSIK QAWQQNETTL 

       550        560 
IELKVPDREG AECLQQLLKQ VAML 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX571869 Genomic DNA. Translation: CAE15447.1.
RefSeqNP_930305.1. NC_005126.1.

3D structure databases

ProteinModelPortalQ7N2K3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243265.plu3073.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE15447; CAE15447; plu3073.
GeneID2803084.
KEGGplu:plu3073.
PATRIC20510496. VBIPhoLum48522_3487.

Organism-specific databases

GenoListplu3073.
CMRSearch...

Phylogenomic databases

eggNOGCOG1165.
HOGENOMHOG000218360.
KOK02551.
OMADGGGIFH.
OrthoDBEOG6NWBQW.
ProtClustDBPRK07449.

Enzyme and pathway databases

UniPathwayUPA00079; UER00164.

Family and domain databases

HAMAPMF_01659. MenD.
InterProIPR004433. MenaQ_synth_MenD.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamPF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFPIRSF004983. MenD. 1 hit.
TIGRFAMsTIGR00173. menD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMEND_PHOLL
AccessionPrimary (citable) accession number: Q7N2K3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: December 15, 2003
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways