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Q7N288

- FADJ_PHOLL

UniProt

Q7N288 - FADJ_PHOLL

Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Photorhabdus luminescens subsp. laumondii (strain TT01)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 2 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

    Catalytic activityi

    (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
    (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei133 – 1331Important for catalytic activityUniRule annotation
    Sitei155 – 1551Important for catalytic activityUniRule annotation

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
    2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
    3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro

    GO - Biological processi

    1. fatty acid beta-oxidation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase, Lyase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fatty acid oxidation complex subunit alphaUniRule annotation
    Including the following 2 domains:
    Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
    3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
    Gene namesi
    Name:fadJUniRule annotation
    Ordered Locus Names:plu3200
    OrganismiPhotorhabdus luminescens subsp. laumondii (strain TT01)
    Taxonomic identifieri243265 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePhotorhabdus
    ProteomesiUP000002514: Chromosome

    Organism-specific databases

    GenoListiplu3200.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 727727Fatty acid oxidation complex subunit alphaPRO_0000109303Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

    Protein-protein interaction databases

    STRINGi243265.plu3200.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7N288.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni16 – 205190Enoyl-CoA hydrataseUniRule annotationAdd
    BLAST
    Regioni321 – 7274073-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
    In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1250.
    HOGENOMiHOG000261346.
    KOiK01782.
    OrthoDBiEOG6M9F0M.

    Family and domain databases

    Gene3Di1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPiMF_01617. FadJ.
    InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsiTIGR02440. FadJ. 1 hit.
    PROSITEiPS00067. 3HCDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7N288-1 [UniParc]FASTAAdd to Basket

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    MTQAQHDAAP ITTGTNQTAS VFSFDVRPDK IGIITINVPG EKVNTLKAEF    50
    VDQFLNVFKQ AQQSSGLKGL ILISGKPDTF IAGADISMIA GCKTKEDARD 100
    LAEKGQKLFS QIANYPLPVV AAIHGACLGG GLELALACHW RVCSQDDKTR 150
    LGLPEVQLGL LPGSGGTQRL PRLIGVSSAL DIMLTGKQLR AKQALRLGLV 200
    DDAVPLDILL DIAIEKVKKG IPVRKPLPWQ QRLLVGPVGR YFLFNIVRKK 250
    TLAKTRGHYP APERIIEVVK EGLEKGMSQG LRAEAVAFGE LAMTRESAAL 300
    RNLFFAATSL KNETGSSEKP AKIKHVGILG GGLMGGGIAN VTATRGKLPV 350
    RIKDINEKGI SQVLKYTWDL LSKRVKQKRL RPAERAQQMM LISGTTDYRG 400
    FAQTDIVVEA VFEDLSLKQK MVAEIETNAK PETIFASNTS SLPIHQIAEK 450
    AQRPEQVIGL HYFSPVDKMP LVEVIPHQGT SEKTIATAVS LAKKQGKTAI 500
    VVGDKAGFYV NRILVPYISE AAHCLVAGEP IDHIDGALVN FGFPVGPINL 550
    LDEVGIDVGT KIMPVLVEQL GPRFAAPESL DAVLKDGRKG RKNGRGFYLY 600
    APGPRKFWQF GKRDKKVDSS VYTLLNITPE SHMLSSEIAQ RCVMLMLNEA 650
    VRCLDEGIIR SPRDGDIGAV FGIGFPPFFG GPFRYIDSLG CARVVEILRR 700
    LESQYGDRFV PCECLVNMAE QNKSFYP 727
    Length:727
    Mass (Da):79,265
    Last modified:December 6, 2005 - v2
    Checksum:i5173D0EE51FB2930
    GO

    Sequence cautioni

    The sequence CAE15574.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571869 Genomic DNA. Translation: CAE15574.1. Different initiation.
    RefSeqiNP_930429.2. NC_005126.1.

    Genome annotation databases

    EnsemblBacteriaiCAE15574; CAE15574; plu3200.
    GeneIDi2803211.
    KEGGiplu:plu3200.
    PATRICi20510816. VBIPhoLum48522_3649.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571869 Genomic DNA. Translation: CAE15574.1 . Different initiation.
    RefSeqi NP_930429.2. NC_005126.1.

    3D structure databases

    ProteinModelPortali Q7N288.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243265.plu3200.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE15574 ; CAE15574 ; plu3200 .
    GeneIDi 2803211.
    KEGGi plu:plu3200.
    PATRICi 20510816. VBIPhoLum48522_3649.

    Organism-specific databases

    GenoListi plu3200.

    Phylogenomic databases

    eggNOGi COG1250.
    HOGENOMi HOG000261346.
    KOi K01782.
    OrthoDBi EOG6M9F0M.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .

    Family and domain databases

    Gene3Di 1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPi MF_01617. FadJ.
    InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsi TIGR02440. FadJ. 1 hit.
    PROSITEi PS00067. 3HCDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TT01.

    Entry informationi

    Entry nameiFADJ_PHOLL
    AccessioniPrimary (citable) accession number: Q7N288
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3