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Q7N288

- FADJ_PHOLL

UniProt

Q7N288 - FADJ_PHOLL

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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Photorhabdus luminescens subsp. laumondii (strain TT01)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei133 – 1331Important for catalytic activityUniRule annotation
Sitei155 – 1551Important for catalytic activityUniRule annotation

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
  2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
  3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
  4. NAD binding Source: InterPro

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
Ordered Locus Names:plu3200
OrganismiPhotorhabdus luminescens subsp. laumondii (strain TT01)
Taxonomic identifieri243265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePhotorhabdus
ProteomesiUP000002514: Chromosome

Organism-specific databases

GenoListiplu3200.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727Fatty acid oxidation complex subunit alphaPRO_0000109303Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi243265.plu3200.

Structurei

3D structure databases

ProteinModelPortaliQ7N288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni16 – 205190Enoyl-CoA hydrataseUniRule annotationAdd
BLAST
Regioni321 – 7274073-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261346.
KOiK01782.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01617. FadJ.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7N288-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQAQHDAAP ITTGTNQTAS VFSFDVRPDK IGIITINVPG EKVNTLKAEF
60 70 80 90 100
VDQFLNVFKQ AQQSSGLKGL ILISGKPDTF IAGADISMIA GCKTKEDARD
110 120 130 140 150
LAEKGQKLFS QIANYPLPVV AAIHGACLGG GLELALACHW RVCSQDDKTR
160 170 180 190 200
LGLPEVQLGL LPGSGGTQRL PRLIGVSSAL DIMLTGKQLR AKQALRLGLV
210 220 230 240 250
DDAVPLDILL DIAIEKVKKG IPVRKPLPWQ QRLLVGPVGR YFLFNIVRKK
260 270 280 290 300
TLAKTRGHYP APERIIEVVK EGLEKGMSQG LRAEAVAFGE LAMTRESAAL
310 320 330 340 350
RNLFFAATSL KNETGSSEKP AKIKHVGILG GGLMGGGIAN VTATRGKLPV
360 370 380 390 400
RIKDINEKGI SQVLKYTWDL LSKRVKQKRL RPAERAQQMM LISGTTDYRG
410 420 430 440 450
FAQTDIVVEA VFEDLSLKQK MVAEIETNAK PETIFASNTS SLPIHQIAEK
460 470 480 490 500
AQRPEQVIGL HYFSPVDKMP LVEVIPHQGT SEKTIATAVS LAKKQGKTAI
510 520 530 540 550
VVGDKAGFYV NRILVPYISE AAHCLVAGEP IDHIDGALVN FGFPVGPINL
560 570 580 590 600
LDEVGIDVGT KIMPVLVEQL GPRFAAPESL DAVLKDGRKG RKNGRGFYLY
610 620 630 640 650
APGPRKFWQF GKRDKKVDSS VYTLLNITPE SHMLSSEIAQ RCVMLMLNEA
660 670 680 690 700
VRCLDEGIIR SPRDGDIGAV FGIGFPPFFG GPFRYIDSLG CARVVEILRR
710 720
LESQYGDRFV PCECLVNMAE QNKSFYP
Length:727
Mass (Da):79,265
Last modified:December 6, 2005 - v2
Checksum:i5173D0EE51FB2930
GO

Sequence cautioni

The sequence CAE15574.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571869 Genomic DNA. Translation: CAE15574.1. Different initiation.
RefSeqiNP_930429.2. NC_005126.1.

Genome annotation databases

EnsemblBacteriaiCAE15574; CAE15574; plu3200.
GeneIDi2803211.
KEGGiplu:plu3200.
PATRICi20510816. VBIPhoLum48522_3649.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571869 Genomic DNA. Translation: CAE15574.1 . Different initiation.
RefSeqi NP_930429.2. NC_005126.1.

3D structure databases

ProteinModelPortali Q7N288.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243265.plu3200.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE15574 ; CAE15574 ; plu3200 .
GeneIDi 2803211.
KEGGi plu:plu3200.
PATRICi 20510816. VBIPhoLum48522_3649.

Organism-specific databases

GenoListi plu3200.

Phylogenomic databases

eggNOGi COG1250.
HOGENOMi HOG000261346.
KOi K01782.
OrthoDBi EOG6M9F0M.

Enzyme and pathway databases

UniPathwayi UPA00659 .

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPi MF_01617. FadJ.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsi TIGR02440. FadJ. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TT01.

Entry informationi

Entry nameiFADJ_PHOLL
AccessioniPrimary (citable) accession number: Q7N288
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: October 29, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3