Reviewed, UniProtKB/Swiss-Prot Q7N1Z4 (PUR4_PHOLL)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	plu3317

Organism

Photorhabdus luminescens subsp. laumondii [Complete proteome] [HAMAP]

Taxonomic identifier

141679 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	1295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm. HAMAP MF_00419
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1295	1295	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000264584
Regions
	Domain	1042 – 1295	254	Glutamine amidotransferase type-1
	Nucleotide binding	306 – 317	12	ATP Potential
Sites
	Active site	1135	1	Nucleophile By similarity
	Active site	1260	1	By similarity
	Active site	1262	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7N1Z4-1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: D768C7E94B790CC7	FASTA	1,295	142,784
	10 20 30 40 50 60 MKILRGSPAL SAFRITKLLS VCQEQQLPVN DIYAEYVHFA EINASLSDVD SAKLQQLLKY 70 80 90 100 110 120 GPSLAEHEPQ GTLLLVTPRP GTISPWSSKA TDIAHNCGLS QVVRLERGVA YYIQSGEMSD 130 140 150 160 170 180 TQWQILSSLL HDRMMETVFT QLEQAEKLFS RQQPVPLKRI DILQAGRSAL ETANIELGLA 190 200 210 220 230 240 LASDEIDYLM DAFQKLGRNP TDVELYMFAQ ANSEHCRHKI FNADWIIDSQ AQPKSLFKMI 250 260 270 280 290 300 KNTYEQTPDY VLSAYKDNAA VMEGSAVGRF FASAENGSYD YHQEQAHILM KVETHNHPTA 310 320 330 340 350 360 ISPWPGASTG SGGEIRDEGA TGRGAKPKAG LVGFSVSNLR IPGFEQPWEE DFGKPERIVS 370 380 390 400 410 420 ALDIMMEGPL GGAAFNNEFG RPALLGYFRT YEEKVNSHNG SELRGYHKPI MLAGGIGNIR 430 440 450 460 470 480 DEHVKKGEIS VGAKLIVLGG PSMNIGLGGG AASSMASGQS DADLDFASVQ RDNPEMERRC 490 500 510 520 530 540 QEVIDRCWQL GENNPILFIH DVGAGGLSNA MPELVSDGGR GGRFELRKIL NDEPGMSPLE 550 560 570 580 590 600 VWCNESQERY VLAVAPEQLP LFEEICRRER APYAIIGEAT EERHLLLNDE HFDNQPIDMP 610 620 630 640 650 660 LDVLLGKTPK MLRDVTTLKA SGESLERRDI DLAEAVKRIM HLPAVAEKTF LITIGDRSVT 670 680 690 700 710 720 GMVSRDQMVG PWQIPVADCA VTTASLDSYY GEAMSMGERA PVALLDFAAS ARMAVGEALT 730 740 750 760 770 780 NIASAYIQDL KRIKLSANWM SAAGHPGEDA GLYAAVKAVG EELCPALGLT IPVGKDSMSM 790 800 810 820 830 840 KTRWHDQGEE REMTAPLSLV ITAFARVEDV RRTVTPELST DEDNALLLID LGQGKNTLGG 850 860 870 880 890 900 TALAQVYRLL GNKTADVRSA EQLAGFFNAI QQLIAEQKLL AYHDRSDGGL LVTLAEMAFA 910 920 930 940 950 960 GHCGIEADIS VFDEDILAGL FTEELGAVVQ IRASDRGFVE SILAEHGLAD CVHYLGKAQA 970 980 990 1000 1010 1020 GDDFVIFSGN TEVYRQNRST LRLWWAETTW QMQRLRDNPA CADQEHQAKQ DNQDPGLNVK 1030 1040 1050 1060 1070 1080 LTFDISEDIA APYILQQVRP KVAVLREQGV NSHVEMAAAF HRAGFEAIDV HMSDLLSGRI 1090 1100 1110 1120 1130 1140 GLSQFQTLVA CGGFSYGDVL GAGEGWAKSI LFNERVRDQF AVFFARPDTL ALGVCNGCQM 1150 1160 1170 1180 1190 1200 MSNLRELIPG AEHWPRFVRN RSERFEARFS LVEITDSPSL FLQDMVGSRI PIAVSHGEGQ 1210 1220 1230 1240 1250 1260 VEFRNRQHLE MLESNQLVAL RYVNNYGQVT ENYPANPNGS VNGITAVTSL DGRATVMMPH 1270 1280 1290 PERVSRTVNN SWHPDEWRED GPWMRIFRNA RKQLG

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571870 Genomic DNA. Translation: CAE15691.1.
RefSeq	NP_930539.1.
3D structure databases
SMR	Q7N1Z4. Positions 1-1295.
ModBase	Search...
Genome annotation databases
GeneID	2803329.
GenomeReviews	Gene locus plu3317 in contig BX470251_GR.
KEGG	plu:plu3317.
NMPDR	fig|243265.1.peg.3163.
Organism-specific databases
PhotoList	plu3317.
CMR	Search...
Phylogenomic databases
HOGENOM	Q7N1Z4.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU3317-MON.
BRENDA	6.3.5.3. 308689.
Family and domain databases
HAMAP	MF_00419. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_PHOLL

Accession

Primary (citable) accession number: Q7N1Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	September 1, 2009
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)