Q7MZB7 (PLSB_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	plu4376

Organism

Photorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]

Taxonomic identifier

243265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	818 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 818	818	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_0000195227
Regions
	Motif	305 – 310	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	Q7MZB7 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: DCE3FD98858A0E62	FASTA	818	93,638
	10 20 30 40 50 60 MSSWRKIYYK LLNLPLKILV KSKLIPTDPI TELRLDTTRP ILYVLPYHSK ADLLALRQQC 70 80 90 100 110 120 LEQDLPDPLN LLEIGDTELP SYVFIDNGPR VFRYCAPKQE SVKIFHAYLD LHRNNPNLDI 130 140 150 160 170 180 QLLPVSVMFG RSPGREGQNA PHLRLLNGIQ KFFAILWLGR DSFVRFSNTV SLRYMATEHG 190 200 210 220 230 240 TDKTIAHKLA RVARMHYSRQ RLAAVGPRLP VRQELFNKLL ASKAIEKAVS DEARTKKISH 250 260 270 280 290 300 EKARQNAINM MEEIAANFSY ETVRLSGRVL GWTWNRLYQG INVHNAERIR RLAQDGHELV 310 320 330 340 350 360 YAPCHRSHMD YLLLSYVLYH QGLVPPHIAA GINLNFWPAG PIFRRLGAFF IRRTFKGNKL 370 380 390 400 410 420 YATIFREYLG ELFARGYSVE YFMEGGRSRT GRLLDPKTGT LSMTLQALLR GESRPITIIP 430 440 450 460 470 480 IYIGYEHVME VATYAKELRG ATKEKEGFFQ MIRGLRKLRN LGQGYVNFGE PIPLIQYLNN 490 500 510 520 530 540 HVPSWRDSID PIEFHRPEWF NPTVNQLSEK IMVNINNTAA ANAINLCSTA LLASRQRALT 550 560 570 580 590 600 REQLLEQLDC YIQLMRNAPY ATDVTVPKKT AEELLEHALQ MDKFEVDKDS MGDIIILPRD 610 620 630 640 650 660 RAVLMTYYRN NIQHLLVLPS LIACIVIHHR RISREALLSQ VAIIYPLLKA ELFMRYSKTE 670 680 690 700 710 720 LPEVVNTLIN ELTRQCLICN KEHGMLVLNP ARIRPLQLLA AGIRETLQRY AITLSLLNAN 730 740 750 760 770 780 PVISRGVLEK ESRMLAQRLS VLHGINAPEF FDKAVFTTSV NTLREEGYIS DSGNAITANT 790 800 810 QELYQVLGEL MSPEIRLTIE SVSLPPEHND TEESAREG

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571873 Genomic DNA. Translation: CAE16748.1.
RefSeq	NP_931549.1. NC_005126.1.
3D structure databases
ProteinModelPortal	Q7MZB7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2804392.
GenomeReviews	Gene locus plu4376 in contig BX470251_GR.
KEGG	plu:plu4376.
NMPDR	fig|243265.1.peg.4172.
PATRIC	20513434. VBIPhoLum48522_4958.
Organism-specific databases
GenoList	plu4376.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU4376-MONOMER.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_PHOLL

Accession

Primary (citable) accession number: Q7MZB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program