Reviewed, UniProtKB/Swiss-Prot Q7MZA0 (ACEK_PHOLL)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	plu4394

Organism

Photorhabdus luminescens subsp. laumondii [Complete proteome] [HAMAP]

Taxonomic identifier

141679 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000057902
Regions
	Nucleotide binding	315 – 321	7	ATP By similarity
Sites
	Active site	371	1	By similarity
	Binding site	336	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MZA0-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 6CBD22B967305EB3	FASTA	585	68,791
	10 20 30 40 50 60 MGIDSAHLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKKR INLYDHHVGL 70 80 90 100 110 120 VVEQLKCIHS AFGYDEEYIA KVKVVYTGLL PDYPRFEIAE SFFNSVYCRL FEHRNLTPDK 130 140 150 160 170 180 LFIFTSQPAR RFRDIPRPLS RDFFPNDNLA LMLRTILNDL PLRLPWENLE RDIYYITDYL 190 200 210 220 230 240 QQTFPVGELL QATFQIANEL FYRNKAAWLV GKIRIGGQVY PFLLPIHHNE SGGIFIDTCL 250 260 270 280 290 300 TDYADASIVF GFARSYFMVY APLPAALVEW LREILPAKST SELYMAIGCQ KHGKTEYYRE 310 320 330 340 350 360 YLAFITFSKE QLIIAPGVKG MVMLVFTSPS FDRVFKVIKD QFAPQKEVTQ ERVQECYRLV 370 380 390 400 410 420 KEHDRVGRMA DTQEFENFVI DKARISPELM EELQKEVPEK LEDLGDKILI KHLYMERRMT 430 440 450 460 470 480 PLNIYMEQVE ERKLKDVIEE YGNAIKQLAA ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC 490 500 510 520 530 540 YMTEVNFRDI PPPRYPEDEL AGEPWYSVAP NDVFPEEFRH FLCTDSRIRR YFEEMHSDLF 550 560 570 580 KADYWRALQE RIRSGHVEDV FAYRRKQRFS QRTEIETYSI AKVSA

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571873 Genomic DNA. Translation: CAE16766.1.
RefSeq	NP_931567.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2804410.
GenomeReviews	Gene locus plu4394 in contig BX470251_GR.
KEGG	plu:plu4394.
NMPDR	fig|243265.1.peg.4190.
Organism-specific databases
PhotoList	plu4394.
CMR	Search...
Phylogenomic databases
HOGENOM	Q7MZA0.
OMA	FYRDFLH.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU4394-MON.
BRENDA	2.7.11.5. 308689.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_PHOLL

Accession

Primary (citable) accession number: Q7MZA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	December 15, 2003
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)