Q7MWK6 (ISPH_PORGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	PG_0604

Organism

Porphyromonas gingivalis (strain ATCC BAA-308 / W83) [Complete proteome] [HAMAP]

Taxonomic identifier

242619 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Porphyromonas

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_0000128852

Sequences

	Sequence		Length	Mass (Da)
	Q7MWK6 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: F0482658CC742398	FASTA	289	31,593
	10 20 30 40 50 60 MIPIEIDSGS GFCFGVVNAI RHAEKQLEKS SDKLYCLGDI VHNTLEVERL GKKGLETIDY 70 80 90 100 110 120 DAFSRLRGAK VLLRAHGEPP EIYRMAGENG VTIIDATCPV VLRLQNKIKS RYEATRSLGA 130 140 150 160 170 180 QVVIYGKRGH AEVNGLVGQT EGTAIVIESE EELDKIDYTR PVILFSQTTK SLEGFGQIID 190 200 210 220 230 240 SISTRMQPGV TFEHHDTICR QVANRIPHIG AFATAHELVF FVAGEKSSNG KVLFGHCLAA 250 260 270 280 NPRSIFISSP EVIVPDMLVP LPASIGICGA TSTPRWQMEE VASHIKALL

References

[1]

"Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83." Nelson K.E., Fleischmann R.D., DeBoy R.T., Paulsen I.T., Fouts D.E., Eisen J.A., Daugherty S.C., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Nelson W.C., Mason T.M., Tallon L., Gray J., Granger D., Tettelin H. , Dong H., Galvin J.L., Duncan M.J., Dewhirst F.E., Fraser C.M. J. Bacteriol. 185:5591-5601(2003) [PubMed: 12949112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-308 / W83.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015924 Genomic DNA. Translation: AAQ65788.1.
RefSeq	NP_904889.1. NC_002950.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2552442.
GenomeReviews	Gene locus PG_0604 in contig AE015924_GR.
KEGG	pgi:PG0604.
NMPDR	fig|242619.1.peg.530.
PATRIC	22978290. VBIPorGin134034_0551.
TIGR	PG_0604.
Phylogenomic databases
HOGENOM	HBG335228.
OMA	HNQGKRY.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	PGIN242619:PG_0604-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_PORGI

Accession

Primary (citable) accession number: Q7MWK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program