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Q7MVS9 (TRMB_PORGI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:PG_0960
OrganismPorphyromonas gingivalis (strain ATCC BAA-308 / W83) [Complete proteome] [HAMAP]
Taxonomic identifier242619 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesPorphyromonadaceaePorphyromonas

Protein attributes

Sequence length244 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 244244tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171371

Regions

Region191 – 1944Substrate binding By similarity

Sites

Active site1161 By similarity
Binding site421S-adenosyl-L-methionine By similarity
Binding site671S-adenosyl-L-methionine By similarity
Binding site941S-adenosyl-L-methionine By similarity
Binding site1161S-adenosyl-L-methionine By similarity
Binding site1201Substrate By similarity
Binding site1501Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7MVS9 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 3F1216F3B456D4A9

FASTA24428,215
        10         20         30         40         50         60 
METFPHVFQY PFAVLQQQES GFPLRGRWHT DFFHNDHPIV LELGCGRGEY TVGLGKRFPE 

        70         80         90        100        110        120 
KNFIGIDIKG ARMWAGAKES LQEGMSNVAF LRTDIELLDR FFAEGEVAEI WITFPDPQMK 

       130        140        150        160        170        180 
KVGKRLTGTR FLSLYDKVLE RGGRIHLKTD SPFLYTYTKA LVELNGLPVH EITDDLYGKG 

       190        200        210        220        230        240 
CVENEILGIR TYYEQQWLER GLTIKYISFG LGEPDCEYRE PDIEIEPDSY RSYNRSRRSQ 


AVPS 

« Hide

References

[1]"Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83."
Nelson K.E., Fleischmann R.D., DeBoy R.T., Paulsen I.T., Fouts D.E., Eisen J.A., Daugherty S.C., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Nelson W.C., Mason T.M., Tallon L., Gray J., Granger D., Tettelin H. expand/collapse author list , Dong H., Galvin J.L., Duncan M.J., Dewhirst F.E., Fraser C.M.
J. Bacteriol. 185:5591-5601(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-308 / W83.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015924 Genomic DNA. Translation: AAQ66090.1.
RefSeqNP_905191.1. NC_002950.2.

3D structure databases

ProteinModelPortalQ7MVS9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING242619.PG0960.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAQ66090; AAQ66090; PG_0960.
GeneID2552988.
KEGGpgi:PG0960.
PATRIC22978980. VBIPorGin134034_0888.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMAGCGKGEY.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycPGIN242619:GHX8-871-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_PORGI
AccessionPrimary (citable) accession number: Q7MVS9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 15, 2003
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways