ID   FTHS_PORGI              Reviewed;         555 AA.
AC   Q7MUZ8;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   27-MAR-2024, entry version 109.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=PG_1321;
OS   Porphyromonas gingivalis (strain ATCC BAA-308 / W83).
OC   Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Porphyromonadaceae;
OC   Porphyromonas.
OX   NCBI_TaxID=242619;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-308 / W83;
RX   PubMed=12949112; DOI=10.1128/jb.185.18.5591-5601.2003;
RA   Nelson K.E., Fleischmann R.D., DeBoy R.T., Paulsen I.T., Fouts D.E.,
RA   Eisen J.A., Daugherty S.C., Dodson R.J., Durkin A.S., Gwinn M.L.,
RA   Haft D.H., Kolonay J.F., Nelson W.C., Mason T.M., Tallon L., Gray J.,
RA   Granger D., Tettelin H., Dong H., Galvin J.L., Duncan M.J., Dewhirst F.E.,
RA   Fraser C.M.;
RT   "Complete genome sequence of the oral pathogenic bacterium Porphyromonas
RT   gingivalis strain W83.";
RL   J. Bacteriol. 185:5591-5601(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; AE015924; AAQ66392.1; -; Genomic_DNA.
DR   RefSeq; WP_004585540.1; NC_002950.2.
DR   AlphaFoldDB; Q7MUZ8; -.
DR   SMR; Q7MUZ8; -.
DR   STRING; 242619.PG_1321; -.
DR   EnsemblBacteria; AAQ66392; AAQ66392; PG_1321.
DR   GeneID; 29256317; -.
DR   KEGG; pgi:PG_1321; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_10; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000000588; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..555
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199368"
SQ   SEQUENCE   555 AA;  60075 MW;  900DD71B42D28A9F CRC64;
     MKSDIQIARD IELQRIEQIA ESIDLPVEQL EPYGRYTAKV PLSCIDEEKV KKGNLILVTA
     ITPNKAGVGK TTVSIGLALG LNHIGKKAIV ALREPSLGPC FGMKGGAAGG GYAQVLPMEN
     INLHFTGDFH AVTSAHNMIT ALLENYIYQN RNTCDGLSEI LWKRVLDVND RSLRNAVTGL
     GTISDGIPRQ TGFDITPASE IMAILCLAKD FEDLRSRLEN ILLGYTKEGA PFTVKDLGIA
     GSIAVLLKDA IKPNLVQTTE HTPAFVHGGP FANIAHGCNS ILATKMALSF GEYAVTEAGF
     GADLGAEKFL DIKCREMGVA PKLTVLVATL RALKLHGGVA ETEIKAPNAE ALRRGLSNLD
     RHIYNLKKFG QQVIVAFNRF DTDEEEEISI VREHCIGQNV GFAVNNAFAE GGKGAEELAK
     LVVEMVENKP SQPLKYAYEP ENPVKMKIEK IAKEIYSAGS VVYSSKADGK LKKIAMQSLD
     HLPVCIAKTQ YSFSSDPKAK GDVRGFELKV SDIIINRGAG MLVVIIGEIM RMPGLPKEPQ
     AVHIDIVDGF IEGLS
//