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Q7MUD3

- SYI_PORGI

UniProt

Q7MUD3 - SYI_PORGI

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Protein

Isoleucine--tRNA ligase

Gene
ileS, PG_1596
Organism
Porphyromonas gingivalis (strain ATCC BAA-308 / W83)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei691 – 6911ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPGIN242619:GHX8-1439-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PG_1596
OrganismiPorphyromonas gingivalis (strain ATCC BAA-308 / W83)
Taxonomic identifieri242619 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesPorphyromonadaceaePorphyromonas
ProteomesiUP000000588: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11371137Isoleucine--tRNA ligaseUniRule annotationPRO_0000098554Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi242619.PG1596.

Structurei

3D structure databases

ProteinModelPortaliQ7MUD3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionUniRule annotationAdd
BLAST
Motifi688 – 6925"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKWIISEI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7MUD3-1 [UniParc]FASTAAdd to Basket

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MSRRFAEYES LDLSRVNEEV LADWMQHRLF EESLKSREGA PSFVFYEGPP     50
SANGMPGIHH VMARAIKDTI CRYKTMKGFR VDRKAGWDTH GLPVELGVEK 100
SLGITKEDIG KSISVEEYNA ACRRDVMKFT KEWEDLTHKM GYWVDMEHPY 150
ITYDNRYIET LWWLLAELYK KGLLYKGYTI QPYSPAAGTG LSTHELNQPG 200
CYRDVKDTTC VAQFKIMDPK PEMQLHGDAF FLAWTTTPWT LPSNTALCVG 250
PEIEYLAVQT FNPYNGIPIT VVLGKPLLHT LFNPKGECEE IPASYDPAQK 300
LLPYKVIASW KGKELEGMRY EQLIPWVNPG EGAFRVITGD FVTTEDGTGI 350
VHIAPTFGAD DDRVAKKSGV PPLMLRDKEG NMRPMVDLAG RYFPTTDLDP 400
VFVEKHMDLP LYDVYAGRYV KNAYDAGKTE KDETLDVELC VMLKMQNRVF 450
RIEKMTHNYP HCWRTDKPVL YYPLDSWFIR TTACKEEMIA NNGKIYWKPE 500
STGTGRFGKW LENLQDWNLS RSRYWGTPLP IWRTEDGSEE ICIGSVEELY 550
NEIEKAVKAG MMERNPWAGF KPGVYTEENY AKIDLHRPFV DGITLCSPSG 600
QPMRRELDLI DVWFDSGAMP YAQMHYPFEN RERVEDGSVF PADFIAEGVD 650
QTRGWFFTLH AIATMISGTS SFKVVVSNGL VLDKKGNKMS KRLGNAVDPF 700
ETIKKYGSDP LRWYMITNSS PWDNLKFDTD GVEEVRRKFF GTLYNTYQFF 750
ALYANLDGFT GEEESIPFAK RPEIDRWILS ELNTLIREVD DQLSDYEPTR 800
AGRAISDFVS ENLSNWYVRL SRRRFWAGDM TEDKLSAYQT LYTSLLTVSK 850
LMAPISPFYA DRLYRDLTGK DESVHLALFP RPDQSQVDRA LEQSMQMAQQ 900
ISSMVLALRR RVNLKVRQPL ATLMIPAIDD EQRRCIESVQ PLILSEVNVK 950
ELRFVDDSMG ILVKRIKPDF KRLGPRYGKV MKALAEAVTA MTQEEIRSLE 1000
KAGTFRMEVA GTPVELELAD VEIVSEDIPG WLVANEGNLT VALDITVTDE 1050
LRSEGLAREL VNRVQNIRKQ SGFEVSDKVD VLLLSNDIMD KVVAEHHDYI 1100
AQQIQAESLE ISDAVSDGVE LDFDDFVLSI QVVKHQG 1137
Length:1,137
Mass (Da):129,945
Last modified:December 15, 2003 - v1
Checksum:i2952F7799895B543
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015924 Genomic DNA. Translation: AAQ66625.1.
RefSeqiNP_905726.1. NC_002950.2.

Genome annotation databases

EnsemblBacteriaiAAQ66625; AAQ66625; PG_1596.
GeneIDi2552895.
KEGGipgi:PG1596.
PATRICi22980196. VBIPorGin134034_1479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015924 Genomic DNA. Translation: AAQ66625.1 .
RefSeqi NP_905726.1. NC_002950.2.

3D structure databases

ProteinModelPortali Q7MUD3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 242619.PG1596.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAQ66625 ; AAQ66625 ; PG_1596 .
GeneIDi 2552895.
KEGGi pgi:PG1596.
PATRICi 22980196. VBIPorGin134034_1479.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KWIISEI.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PGIN242619:GHX8-1439-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-308 / W83.

Entry informationi

Entry nameiSYI_PORGI
AccessioniPrimary (citable) accession number: Q7MUD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 15, 2003
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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