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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Porphyromonas gingivalis (strain ATCC BAA-308 / W83)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei691ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPGIN242619:G1G02-1490-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PG_1596
OrganismiPorphyromonas gingivalis (strain ATCC BAA-308 / W83)
Taxonomic identifieri242619 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesPorphyromonadaceaePorphyromonas
Proteomesi
  • UP000000588 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985541 – 1137Isoleucine--tRNA ligaseAdd BLAST1137

Proteomic databases

PRIDEiQ7MUD3

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi242619.PG1596

Structurei

3D structure databases

ProteinModelPortaliQ7MUD3
SMRiQ7MUD3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi50 – 60"HIGH" regionAdd BLAST11
Motifi688 – 692"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 3 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 2 hits
TIGRFAMsiTIGR00392 ileS, 1 hit

Sequencei

Sequence statusi: Complete.

Q7MUD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRFAEYES LDLSRVNEEV LADWMQHRLF EESLKSREGA PSFVFYEGPP
60 70 80 90 100
SANGMPGIHH VMARAIKDTI CRYKTMKGFR VDRKAGWDTH GLPVELGVEK
110 120 130 140 150
SLGITKEDIG KSISVEEYNA ACRRDVMKFT KEWEDLTHKM GYWVDMEHPY
160 170 180 190 200
ITYDNRYIET LWWLLAELYK KGLLYKGYTI QPYSPAAGTG LSTHELNQPG
210 220 230 240 250
CYRDVKDTTC VAQFKIMDPK PEMQLHGDAF FLAWTTTPWT LPSNTALCVG
260 270 280 290 300
PEIEYLAVQT FNPYNGIPIT VVLGKPLLHT LFNPKGECEE IPASYDPAQK
310 320 330 340 350
LLPYKVIASW KGKELEGMRY EQLIPWVNPG EGAFRVITGD FVTTEDGTGI
360 370 380 390 400
VHIAPTFGAD DDRVAKKSGV PPLMLRDKEG NMRPMVDLAG RYFPTTDLDP
410 420 430 440 450
VFVEKHMDLP LYDVYAGRYV KNAYDAGKTE KDETLDVELC VMLKMQNRVF
460 470 480 490 500
RIEKMTHNYP HCWRTDKPVL YYPLDSWFIR TTACKEEMIA NNGKIYWKPE
510 520 530 540 550
STGTGRFGKW LENLQDWNLS RSRYWGTPLP IWRTEDGSEE ICIGSVEELY
560 570 580 590 600
NEIEKAVKAG MMERNPWAGF KPGVYTEENY AKIDLHRPFV DGITLCSPSG
610 620 630 640 650
QPMRRELDLI DVWFDSGAMP YAQMHYPFEN RERVEDGSVF PADFIAEGVD
660 670 680 690 700
QTRGWFFTLH AIATMISGTS SFKVVVSNGL VLDKKGNKMS KRLGNAVDPF
710 720 730 740 750
ETIKKYGSDP LRWYMITNSS PWDNLKFDTD GVEEVRRKFF GTLYNTYQFF
760 770 780 790 800
ALYANLDGFT GEEESIPFAK RPEIDRWILS ELNTLIREVD DQLSDYEPTR
810 820 830 840 850
AGRAISDFVS ENLSNWYVRL SRRRFWAGDM TEDKLSAYQT LYTSLLTVSK
860 870 880 890 900
LMAPISPFYA DRLYRDLTGK DESVHLALFP RPDQSQVDRA LEQSMQMAQQ
910 920 930 940 950
ISSMVLALRR RVNLKVRQPL ATLMIPAIDD EQRRCIESVQ PLILSEVNVK
960 970 980 990 1000
ELRFVDDSMG ILVKRIKPDF KRLGPRYGKV MKALAEAVTA MTQEEIRSLE
1010 1020 1030 1040 1050
KAGTFRMEVA GTPVELELAD VEIVSEDIPG WLVANEGNLT VALDITVTDE
1060 1070 1080 1090 1100
LRSEGLAREL VNRVQNIRKQ SGFEVSDKVD VLLLSNDIMD KVVAEHHDYI
1110 1120 1130
AQQIQAESLE ISDAVSDGVE LDFDDFVLSI QVVKHQG
Length:1,137
Mass (Da):129,945
Last modified:December 15, 2003 - v1
Checksum:i2952F7799895B543
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015924 Genomic DNA Translation: AAQ66625.1
RefSeqiWP_005874631.1, NC_002950.2

Genome annotation databases

EnsemblBacteriaiAAQ66625; AAQ66625; PG_1596
KEGGipgi:PG_1596
PATRICifig|242619.8.peg.1479

Similar proteinsi

Entry informationi

Entry nameiSYI_PORGI
AccessioniPrimary (citable) accession number: Q7MUD3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 15, 2003
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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