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Q7MUD3

- SYI_PORGI

UniProt

Q7MUD3 - SYI_PORGI

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Porphyromonas gingivalis (strain ATCC BAA-308 / W83)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (15 Dec 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei691 – 6911ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPGIN242619:GHX8-1439-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PG_1596
    OrganismiPorphyromonas gingivalis (strain ATCC BAA-308 / W83)
    Taxonomic identifieri242619 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesPorphyromonadaceaePorphyromonas
    ProteomesiUP000000588: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11371137Isoleucine--tRNA ligasePRO_0000098554Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi242619.PG1596.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7MUD3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi50 – 6011"HIGH" regionAdd
    BLAST
    Motifi688 – 6925"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKWIISEI.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7MUD3-1 [UniParc]FASTAAdd to Basket

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    MSRRFAEYES LDLSRVNEEV LADWMQHRLF EESLKSREGA PSFVFYEGPP     50
    SANGMPGIHH VMARAIKDTI CRYKTMKGFR VDRKAGWDTH GLPVELGVEK 100
    SLGITKEDIG KSISVEEYNA ACRRDVMKFT KEWEDLTHKM GYWVDMEHPY 150
    ITYDNRYIET LWWLLAELYK KGLLYKGYTI QPYSPAAGTG LSTHELNQPG 200
    CYRDVKDTTC VAQFKIMDPK PEMQLHGDAF FLAWTTTPWT LPSNTALCVG 250
    PEIEYLAVQT FNPYNGIPIT VVLGKPLLHT LFNPKGECEE IPASYDPAQK 300
    LLPYKVIASW KGKELEGMRY EQLIPWVNPG EGAFRVITGD FVTTEDGTGI 350
    VHIAPTFGAD DDRVAKKSGV PPLMLRDKEG NMRPMVDLAG RYFPTTDLDP 400
    VFVEKHMDLP LYDVYAGRYV KNAYDAGKTE KDETLDVELC VMLKMQNRVF 450
    RIEKMTHNYP HCWRTDKPVL YYPLDSWFIR TTACKEEMIA NNGKIYWKPE 500
    STGTGRFGKW LENLQDWNLS RSRYWGTPLP IWRTEDGSEE ICIGSVEELY 550
    NEIEKAVKAG MMERNPWAGF KPGVYTEENY AKIDLHRPFV DGITLCSPSG 600
    QPMRRELDLI DVWFDSGAMP YAQMHYPFEN RERVEDGSVF PADFIAEGVD 650
    QTRGWFFTLH AIATMISGTS SFKVVVSNGL VLDKKGNKMS KRLGNAVDPF 700
    ETIKKYGSDP LRWYMITNSS PWDNLKFDTD GVEEVRRKFF GTLYNTYQFF 750
    ALYANLDGFT GEEESIPFAK RPEIDRWILS ELNTLIREVD DQLSDYEPTR 800
    AGRAISDFVS ENLSNWYVRL SRRRFWAGDM TEDKLSAYQT LYTSLLTVSK 850
    LMAPISPFYA DRLYRDLTGK DESVHLALFP RPDQSQVDRA LEQSMQMAQQ 900
    ISSMVLALRR RVNLKVRQPL ATLMIPAIDD EQRRCIESVQ PLILSEVNVK 950
    ELRFVDDSMG ILVKRIKPDF KRLGPRYGKV MKALAEAVTA MTQEEIRSLE 1000
    KAGTFRMEVA GTPVELELAD VEIVSEDIPG WLVANEGNLT VALDITVTDE 1050
    LRSEGLAREL VNRVQNIRKQ SGFEVSDKVD VLLLSNDIMD KVVAEHHDYI 1100
    AQQIQAESLE ISDAVSDGVE LDFDDFVLSI QVVKHQG 1137
    Length:1,137
    Mass (Da):129,945
    Last modified:December 15, 2003 - v1
    Checksum:i2952F7799895B543
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015924 Genomic DNA. Translation: AAQ66625.1.
    RefSeqiNP_905726.1. NC_002950.2.

    Genome annotation databases

    EnsemblBacteriaiAAQ66625; AAQ66625; PG_1596.
    GeneIDi2552895.
    KEGGipgi:PG1596.
    PATRICi22980196. VBIPorGin134034_1479.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015924 Genomic DNA. Translation: AAQ66625.1 .
    RefSeqi NP_905726.1. NC_002950.2.

    3D structure databases

    ProteinModelPortali Q7MUD3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 242619.PG1596.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAQ66625 ; AAQ66625 ; PG_1596 .
    GeneIDi 2552895.
    KEGGi pgi:PG1596.
    PATRICi 22980196. VBIPorGin134034_1479.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KWIISEI.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PGIN242619:GHX8-1439-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-308 / W83.

    Entry informationi

    Entry nameiSYI_PORGI
    AccessioniPrimary (citable) accession number: Q7MUD3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 15, 2003
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3