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Q7MUD3

- SYI_PORGI

UniProt

Q7MUD3 - SYI_PORGI

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Porphyromonas gingivalis (strain ATCC BAA-308 / W83)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei691 – 6911ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPGIN242619:GHX8-1439-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PG_1596
OrganismiPorphyromonas gingivalis (strain ATCC BAA-308 / W83)
Taxonomic identifieri242619 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesPorphyromonadaceaePorphyromonas
ProteomesiUP000000588: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11371137Isoleucine--tRNA ligasePRO_0000098554Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi242619.PG1596.

Structurei

3D structure databases

ProteinModelPortaliQ7MUD3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionAdd
BLAST
Motifi688 – 6925"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKWIISEI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7MUD3 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRRFAEYES LDLSRVNEEV LADWMQHRLF EESLKSREGA PSFVFYEGPP
60 70 80 90 100
SANGMPGIHH VMARAIKDTI CRYKTMKGFR VDRKAGWDTH GLPVELGVEK
110 120 130 140 150
SLGITKEDIG KSISVEEYNA ACRRDVMKFT KEWEDLTHKM GYWVDMEHPY
160 170 180 190 200
ITYDNRYIET LWWLLAELYK KGLLYKGYTI QPYSPAAGTG LSTHELNQPG
210 220 230 240 250
CYRDVKDTTC VAQFKIMDPK PEMQLHGDAF FLAWTTTPWT LPSNTALCVG
260 270 280 290 300
PEIEYLAVQT FNPYNGIPIT VVLGKPLLHT LFNPKGECEE IPASYDPAQK
310 320 330 340 350
LLPYKVIASW KGKELEGMRY EQLIPWVNPG EGAFRVITGD FVTTEDGTGI
360 370 380 390 400
VHIAPTFGAD DDRVAKKSGV PPLMLRDKEG NMRPMVDLAG RYFPTTDLDP
410 420 430 440 450
VFVEKHMDLP LYDVYAGRYV KNAYDAGKTE KDETLDVELC VMLKMQNRVF
460 470 480 490 500
RIEKMTHNYP HCWRTDKPVL YYPLDSWFIR TTACKEEMIA NNGKIYWKPE
510 520 530 540 550
STGTGRFGKW LENLQDWNLS RSRYWGTPLP IWRTEDGSEE ICIGSVEELY
560 570 580 590 600
NEIEKAVKAG MMERNPWAGF KPGVYTEENY AKIDLHRPFV DGITLCSPSG
610 620 630 640 650
QPMRRELDLI DVWFDSGAMP YAQMHYPFEN RERVEDGSVF PADFIAEGVD
660 670 680 690 700
QTRGWFFTLH AIATMISGTS SFKVVVSNGL VLDKKGNKMS KRLGNAVDPF
710 720 730 740 750
ETIKKYGSDP LRWYMITNSS PWDNLKFDTD GVEEVRRKFF GTLYNTYQFF
760 770 780 790 800
ALYANLDGFT GEEESIPFAK RPEIDRWILS ELNTLIREVD DQLSDYEPTR
810 820 830 840 850
AGRAISDFVS ENLSNWYVRL SRRRFWAGDM TEDKLSAYQT LYTSLLTVSK
860 870 880 890 900
LMAPISPFYA DRLYRDLTGK DESVHLALFP RPDQSQVDRA LEQSMQMAQQ
910 920 930 940 950
ISSMVLALRR RVNLKVRQPL ATLMIPAIDD EQRRCIESVQ PLILSEVNVK
960 970 980 990 1000
ELRFVDDSMG ILVKRIKPDF KRLGPRYGKV MKALAEAVTA MTQEEIRSLE
1010 1020 1030 1040 1050
KAGTFRMEVA GTPVELELAD VEIVSEDIPG WLVANEGNLT VALDITVTDE
1060 1070 1080 1090 1100
LRSEGLAREL VNRVQNIRKQ SGFEVSDKVD VLLLSNDIMD KVVAEHHDYI
1110 1120 1130
AQQIQAESLE ISDAVSDGVE LDFDDFVLSI QVVKHQG
Length:1,137
Mass (Da):129,945
Last modified:December 15, 2003 - v1
Checksum:i2952F7799895B543
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015924 Genomic DNA. Translation: AAQ66625.1.
RefSeqiNP_905726.1. NC_002950.2.

Genome annotation databases

EnsemblBacteriaiAAQ66625; AAQ66625; PG_1596.
GeneIDi2552895.
KEGGipgi:PG1596.
PATRICi22980196. VBIPorGin134034_1479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015924 Genomic DNA. Translation: AAQ66625.1 .
RefSeqi NP_905726.1. NC_002950.2.

3D structure databases

ProteinModelPortali Q7MUD3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 242619.PG1596.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAQ66625 ; AAQ66625 ; PG_1596 .
GeneIDi 2552895.
KEGGi pgi:PG1596.
PATRICi 22980196. VBIPorGin134034_1479.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KWIISEI.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PGIN242619:GHX8-1439-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-308 / W83.

Entry informationi

Entry nameiSYI_PORGI
AccessioniPrimary (citable) accession number: Q7MUD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 15, 2003
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3