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Q7MST9 (TRMB_WOLSU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:WS0145
OrganismWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes) [Complete proteome] [HAMAP]
Taxonomic identifier273121 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella

Protein attributes

Sequence length388 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

tRNA methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 388388tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171423

Sites

Binding site1291S-adenosyl-L-methionine By similarity
Binding site1541S-adenosyl-L-methionine By similarity
Binding site1811S-adenosyl-L-methionine By similarity
Binding site2071Substrate By similarity
Binding site2371Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7MST9 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: B4D488C938188155

FASTA38845,635
        10         20         30         40         50         60 
MPHFIASSFT HPDYPFEEEG YCFSDSLSNL LHPHEELVRV EVEGKEFFLR IKHRLKENDF 

        70         80         90        100        110        120 
IIRFDKSTRV SPVGIIKKAL RIFAQKSQAQ ILSDNLSEED SIRQKRLTPF LKTPEFFMHE 

       130        140        150        160        170        180 
AHQGQYWVEI GFGSGRHLLH QAKAHPEKRF IGLEIHTPSI EQVLRRLELE EISNVSIVSY 

       190        200        210        220        230        240 
DARVFLELLP SNAIERLFVH FPVPWDKKPH RRIFSRAFLN EAIRTLGVGG VLELRTDSED 

       250        260        270        280        290        300 
YFLFAKELLL ELNRVHFSIR KNFDAPISSK YEDRWRKQNK NIYDLLLHND QHSPDPLRPS 

       310        320        330        340        350        360 
DFSFKPLKRC DAAELRREIR EGWFASIERL YRSKDGKSMA IRSSFGDFAY PEKKYLWIKG 

       370        380 
EEARYFGSSP IPSLANHQAH KLLEEWLS 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX571657 Genomic DNA. Translation: CAE09308.1.
RefSeqNP_906408.1. NC_005090.1.

3D structure databases

ProteinModelPortalQ7MST9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273121.WS0145.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE09308; CAE09308; WS0145.
GeneID2553837.
KEGGwsu:WS0145.
PATRIC24037386. VBIWolSuc63014_0140.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000252163.
KOK03439.
OMASKYEARW.
OrthoDBEOG6K6VBC.
ProtClustDBPRK14121.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_WOLSU
AccessionPrimary (citable) accession number: Q7MST9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 15, 2003
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways