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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway:iN(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei129 – 1291S-adenosyl-L-methionineUniRule annotation
Binding sitei154 – 1541S-adenosyl-L-methionineUniRule annotation
Binding sitei181 – 1811S-adenosyl-L-methionineUniRule annotation
Binding sitei207 – 2071SubstrateUniRule annotation
Binding sitei237 – 2371SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:WS0145
OrganismiWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Taxonomic identifieri273121 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella
ProteomesiUP000000422 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388tRNA (guanine-N(7)-)-methyltransferasePRO_0000171423Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi273121.WS0145.

Structurei

3D structure databases

ProteinModelPortaliQ7MST9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000252163.
KOiK03439.
OMAiHFPVPWN.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7MST9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHFIASSFT HPDYPFEEEG YCFSDSLSNL LHPHEELVRV EVEGKEFFLR
60 70 80 90 100
IKHRLKENDF IIRFDKSTRV SPVGIIKKAL RIFAQKSQAQ ILSDNLSEED
110 120 130 140 150
SIRQKRLTPF LKTPEFFMHE AHQGQYWVEI GFGSGRHLLH QAKAHPEKRF
160 170 180 190 200
IGLEIHTPSI EQVLRRLELE EISNVSIVSY DARVFLELLP SNAIERLFVH
210 220 230 240 250
FPVPWDKKPH RRIFSRAFLN EAIRTLGVGG VLELRTDSED YFLFAKELLL
260 270 280 290 300
ELNRVHFSIR KNFDAPISSK YEDRWRKQNK NIYDLLLHND QHSPDPLRPS
310 320 330 340 350
DFSFKPLKRC DAAELRREIR EGWFASIERL YRSKDGKSMA IRSSFGDFAY
360 370 380
PEKKYLWIKG EEARYFGSSP IPSLANHQAH KLLEEWLS
Length:388
Mass (Da):45,635
Last modified:December 15, 2003 - v1
Checksum:iB4D488C938188155
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571657 Genomic DNA. Translation: CAE09308.1.
RefSeqiWP_011138108.1. NC_005090.1.

Genome annotation databases

EnsemblBacteriaiCAE09308; CAE09308; WS0145.
KEGGiwsu:WS0145.
PATRICi24037386. VBIWolSuc63014_0140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571657 Genomic DNA. Translation: CAE09308.1.
RefSeqiWP_011138108.1. NC_005090.1.

3D structure databases

ProteinModelPortaliQ7MST9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273121.WS0145.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE09308; CAE09308; WS0145.
KEGGiwsu:WS0145.
PATRICi24037386. VBIWolSuc63014_0140.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000252163.
KOiK03439.
OMAiHFPVPWN.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.

Entry informationi

Entry nameiTRMB_WOLSU
AccessioniPrimary (citable) accession number: Q7MST9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 15, 2003
Last modified: July 22, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.