Q7MP85 (PDXA_VIBVY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenase
EC=1.1.1.262

Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenase

Gene names

Name:	pdxA
Ordered Locus Names:	VV0479

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio vulnificus

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP) By similarity. HAMAP-Rule MF_00536
Catalytic activity	4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH. HAMAP-Rule MF_00536
Cofactor	Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt By similarity.
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5. HAMAP-Rule MF_00536
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is located at the dimer interface By similarity. HAMAP-Rule MF_00536
Sequence similarities	Belongs to the PdxA family.
Sequence caution	The sequence BAC93243.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	Cobalt Magnesium Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pyridoxal phosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4-hydroxythreonine-4-phosphate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP NAD binding Inferred from electronic annotation. Source: InterPro cobalt ion binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	4-hydroxythreonine-4-phosphate dehydrogenase HAMAP-Rule MF_00536		PRO_0000188836
Sites
	Metal binding	164	1	Divalent metal cation; shared with dimeric partner By similarity
	Metal binding	209	1	Divalent metal cation; shared with dimeric partner By similarity
	Metal binding	265	1	Divalent metal cation; shared with dimeric partner By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	273	1	Substrate By similarity
	Binding site	282	1	Substrate By similarity
	Binding site	291	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MP85 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: 28BC3C09FF777ACB	FASTA	328	35,506
	10 20 30 40 50 60 MVKRLVVTAG EPAGIGPDLV LALSKEHWPH QLVVCADKKM LAQRAEQLGI NVTLLDYDAS 70 80 90 100 110 120 TAPSPQQAGT LVVEHIDMPS TCVAGQLNEE NGHYVLKTLE RAALGCMKSE FDAIVTGPVH 130 140 150 160 170 180 KGVINRAGVA FSGHTEFFAE LSNTPLVVMM LATEGLRVAL VTTHIPLAYV SKAVTAERLQ 190 200 210 220 230 240 KIIDILHRDL VEKFAIAEPK IYVCGLNPHA GEDGCLGREE IETITPTLEK IRQEKGIHLL 250 260 270 280 290 300 GPLPADTIFN EKYLNDADAV LGMYHDQVLP VLKYKGFGQS VNITLGLPFI RTSVDHGTAL 310 320 DLAGTGQANT GSFRTALQHA IELVEKKQ

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YJ016.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000037 Genomic DNA. Translation: BAC93243.1. Different initiation.
RefSeq	NP_933272.1. NC_005139.1.
3D structure databases
ProteinModelPortal	Q7MP85.
SMR	Q7MP85. Positions 3-328.
ModBase	Search...
Protein-protein interaction databases
STRING	196600.VV0479.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC93243; BAC93243; BAC93243.
GeneID	2623246.
KEGG	vvy:VV0479.
PATRIC	20167768. VBIVibVul40472_0505.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1995.
HOGENOM	HOG000221592.
KO	K00097.
OMA	RITHAAM.
ProtClustDB	PRK00232.
Enzyme and pathway databases
UniPathway	UPA00244; UER00312.
Family and domain databases
Gene3D	3.40.718.10. 1 hit.
HAMAP	MF_00536. PdxA.
InterPro	IPR024084. IsoPropMal-DH-like_dom. IPR005255. PyrdxlP_synth_PdxA. [Graphical view]
Pfam	PF04166. PdxA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00557. pdxA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PDXA_VIBVY

Accession

Primary (citable) accession number: Q7MP85

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program