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Q7MNM8

- SYI_VIBVY

UniProt

Q7MNM8 - SYI_VIBVY

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Protein
Isoleucine--tRNA ligase
Gene
ileS, VV0687
Organism
Vibrio vulnificus (strain YJ016)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei566 – 5661Aminoacyl-adenylate By similarity
Binding sitei610 – 6101ATP By similarity
Metal bindingi911 – 9111Zinc By similarity
Metal bindingi914 – 9141Zinc By similarity
Metal bindingi931 – 9311Zinc By similarity
Metal bindingi934 – 9341Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciVVUL196600:GJ9W-707-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:VV0687
OrganismiVibrio vulnificus (strain YJ016)
Taxonomic identifieri196600 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000002675: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 948948Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098501Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi196600.VV0687.

Structurei

3D structure databases

ProteinModelPortaliQ7MNM8.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi607 – 6115"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7MNM8-1 [UniParc]FASTAAdd to Basket

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MSEYKDTLNL PETGFPMRGD LAKREPEMLK RWYQEDLYGE IRKAKKGKKS    50
FVLHDGPPYA NGDIHIGHAL NKILKDIIIK SKTLSGFDAP YVPGWDCHGL 100
PIELMVEKKV GKPGQKVTAA EFREKCREYA AGQVEGQKES FKRLGIMGEW 150
DKPYRTMDFT TEANIIRALG QIADNGHLLK GFKPVHWCTD CGSALAEAEV 200
EYKNKVSPSI DVRFKAADEA ALLAKFSLNE GHQGQGDVSI VIWTTTPWTL 250
PANRAVCLRD DLEYVLIQVE GDNPERIIVA AELAKDVMDR AGIEHFHNLG 300
FAKGADLELT QFQHPFYDFT VPAILGDHVT TDSGTGVVHT APGHGQEDFA 350
VGQKYNLEVA NPVGSNGVYL PDTELFAGQH VFKANDAVVE TLKEKGALLH 400
HHAYEHSYPH CWRHKTPIIF RATPQWFVSM DQAGLRAKAL ESIKGVQWMP 450
EWGQSRIEGM IEGRPEWCIS RQRTWGVPIA LFVHKETAEL HPNTSELIEK 500
VAQVVEQKGI QAWWDIDAAE LLGDDAAQYE KVLDTLDVWF DSGVTHYAVV 550
DKREEFNGAE ADMYLEGSDQ HRGWFQSSLI SSIAMKGKAP YKQVLTHGFV 600
VDGHGRKMSK SIGNVVAPKD VTNKLGADIL RLWVASTDYT GEVAVSDEIL 650
KRSADAYRRI RNTARFFLAN LNGFNPETDI VPVEEMVALD RWAVGRALAA 700
QNEIVKAYDE YNTHAVTQRL MHFCSIEMGS FYLDVIKDRQ YTAKLGGHAQ 750
RSCQTALYYI VEALVRWMAP IMSFTADEIW NQMPASLPSG ESRDKFVFTG 800
EWFDGLFGLA EGEELNNAFW SEMQKVRGAV NKLLEAARSD KTIGGSLQAE 850
LTLFADDALA AKINKLEDEL RFVLLTSAAT VKPLSEKSDA AQATDIEGLF 900
VEVRATEAEK CDRCWHHTPD VGTIEGHEKI CGRCVSNVDG EGEARKFA 948
Length:948
Mass (Da):105,881
Last modified:December 15, 2003 - v1
Checksum:iBA6B0431BE52710E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000037 Genomic DNA. Translation: BAC93451.1.
RefSeqiNP_933480.1. NC_005139.1.

Genome annotation databases

EnsemblBacteriaiBAC93451; BAC93451; BAC93451.
GeneIDi2623456.
KEGGivvy:VV0687.
PATRICi20168174. VBIVibVul40472_0707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000037 Genomic DNA. Translation: BAC93451.1 .
RefSeqi NP_933480.1. NC_005139.1.

3D structure databases

ProteinModelPortali Q7MNM8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196600.VV0687.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC93451 ; BAC93451 ; BAC93451 .
GeneIDi 2623456.
KEGGi vvy:VV0687.
PATRICi 20168174. VBIVibVul40472_0707.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci VVUL196600:GJ9W-707-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YJ016.

Entry informationi

Entry nameiSYI_VIBVY
AccessioniPrimary (citable) accession number: Q7MNM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 15, 2003
Last modified: May 14, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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