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Q7MNM8

- SYI_VIBVY

UniProt

Q7MNM8 - SYI_VIBVY

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Vibrio vulnificus (strain YJ016)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei566 – 5661Aminoacyl-adenylateUniRule annotation
Binding sitei610 – 6101ATPUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi914 – 9141ZincUniRule annotation
Metal bindingi931 – 9311ZincUniRule annotation
Metal bindingi934 – 9341ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciVVUL196600:GJ9W-707-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:VV0687
OrganismiVibrio vulnificus (strain YJ016)
Taxonomic identifieri196600 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000002675: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 948948Isoleucine--tRNA ligasePRO_0000098501Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi196600.VV0687.

Structurei

3D structure databases

ProteinModelPortaliQ7MNM8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi607 – 6115"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7MNM8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEYKDTLNL PETGFPMRGD LAKREPEMLK RWYQEDLYGE IRKAKKGKKS
60 70 80 90 100
FVLHDGPPYA NGDIHIGHAL NKILKDIIIK SKTLSGFDAP YVPGWDCHGL
110 120 130 140 150
PIELMVEKKV GKPGQKVTAA EFREKCREYA AGQVEGQKES FKRLGIMGEW
160 170 180 190 200
DKPYRTMDFT TEANIIRALG QIADNGHLLK GFKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYKNKVSPSI DVRFKAADEA ALLAKFSLNE GHQGQGDVSI VIWTTTPWTL
260 270 280 290 300
PANRAVCLRD DLEYVLIQVE GDNPERIIVA AELAKDVMDR AGIEHFHNLG
310 320 330 340 350
FAKGADLELT QFQHPFYDFT VPAILGDHVT TDSGTGVVHT APGHGQEDFA
360 370 380 390 400
VGQKYNLEVA NPVGSNGVYL PDTELFAGQH VFKANDAVVE TLKEKGALLH
410 420 430 440 450
HHAYEHSYPH CWRHKTPIIF RATPQWFVSM DQAGLRAKAL ESIKGVQWMP
460 470 480 490 500
EWGQSRIEGM IEGRPEWCIS RQRTWGVPIA LFVHKETAEL HPNTSELIEK
510 520 530 540 550
VAQVVEQKGI QAWWDIDAAE LLGDDAAQYE KVLDTLDVWF DSGVTHYAVV
560 570 580 590 600
DKREEFNGAE ADMYLEGSDQ HRGWFQSSLI SSIAMKGKAP YKQVLTHGFV
610 620 630 640 650
VDGHGRKMSK SIGNVVAPKD VTNKLGADIL RLWVASTDYT GEVAVSDEIL
660 670 680 690 700
KRSADAYRRI RNTARFFLAN LNGFNPETDI VPVEEMVALD RWAVGRALAA
710 720 730 740 750
QNEIVKAYDE YNTHAVTQRL MHFCSIEMGS FYLDVIKDRQ YTAKLGGHAQ
760 770 780 790 800
RSCQTALYYI VEALVRWMAP IMSFTADEIW NQMPASLPSG ESRDKFVFTG
810 820 830 840 850
EWFDGLFGLA EGEELNNAFW SEMQKVRGAV NKLLEAARSD KTIGGSLQAE
860 870 880 890 900
LTLFADDALA AKINKLEDEL RFVLLTSAAT VKPLSEKSDA AQATDIEGLF
910 920 930 940
VEVRATEAEK CDRCWHHTPD VGTIEGHEKI CGRCVSNVDG EGEARKFA
Length:948
Mass (Da):105,881
Last modified:December 15, 2003 - v1
Checksum:iBA6B0431BE52710E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000037 Genomic DNA. Translation: BAC93451.1.
RefSeqiNP_933480.1. NC_005139.1.

Genome annotation databases

EnsemblBacteriaiBAC93451; BAC93451; BAC93451.
GeneIDi2623456.
KEGGivvy:VV0687.
PATRICi20168174. VBIVibVul40472_0707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000037 Genomic DNA. Translation: BAC93451.1 .
RefSeqi NP_933480.1. NC_005139.1.

3D structure databases

ProteinModelPortali Q7MNM8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196600.VV0687.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC93451 ; BAC93451 ; BAC93451 .
GeneIDi 2623456.
KEGGi vvy:VV0687.
PATRICi 20168174. VBIVibVul40472_0707.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci VVUL196600:GJ9W-707-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YJ016.

Entry informationi

Entry nameiSYI_VIBVY
AccessioniPrimary (citable) accession number: Q7MNM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 15, 2003
Last modified: November 26, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3