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Protein

Chaperone protein ClpB

Gene

clpB

Organism
Vibrio vulnificus (strain YJ016)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi206 – 213ATP 1By similarity8
Nucleotide bindingi605 – 612ATP 2By similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:VV0715
OrganismiVibrio vulnificus (strain YJ016)
Taxonomic identifieri196600 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002675 Componenti: Chromosome I

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001912031 – 857Chaperone protein ClpBAdd BLAST857

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ7MNK1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 143N-terminalBy similarityAdd BLAST143
Regioni159 – 340NBD1By similarityAdd BLAST182
Regioni341 – 545LinkerBy similarityAdd BLAST205
Regioni555 – 765NBD2By similarityAdd BLAST211
Regioni766 – 857C-terminalBy similarityAdd BLAST92

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili391 – 525By similarityAdd BLAST135

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

HOGENOMiHOG000218211.
KOiK03695.
OMAiIMVEPTS.
OrthoDBiPOG091H019M.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR017730. Chaperonin_ClpB.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR03346. chaperone_ClpB. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7MNK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLDRFTSKF QIAISDAQSL ALGRDHQYIE PVHLMVALLD QNGSPIRPLL
60 70 80 90 100
TILNVDVTHL RSKLSEMLDR LPKVSGIGGD VQLSSAMGAM FNLCDKIAQK
110 120 130 140 150
RQDAYISSEI FLLAAIEDRG PLGQLFKELG LTEQKVSQAI EQIRGGQKVN
160 170 180 190 200
DQNAEELRQA LEKFTIDLTE RAEQGKLDPV IGRDDEIRRT IQVLQRRTKN
210 220 230 240 250
NPVIIGEPGV GKTAIVEGLA QRIINNEVPE GLRGRRVLSL DMGALVAGAK
260 270 280 290 300
YRGEFEERLK SVLNELSKEE GNIILFIDEL HTMVGAGKGE GSMDAGNMLK
310 320 330 340 350
PALARGELHC VGATTLDEYR QYIEKDPALE RRFQKVLVDE PTVEDTIAIL
360 370 380 390 400
RGLKERYELH HHVEITDPAI VAAASLSHRY VSDRQLPDKA IDLIDEAASS
410 420 430 440 450
IRMQIDSKPE ALDKLERKII QLKIEQQALS NEHDEASEKR LRSLNEELNE
460 470 480 490 500
KEREFAELEE IWNAEKAALS GTQHIKAALE QARMDMEFAR RAGDLSRMSE
510 520 530 540 550
LQYGRIPELE KQLDLATQAE MQEMTLLKNK VTDNEIAEVL SKQTGIPVSK
560 570 580 590 600
MLEAEKEKLL RMEEVLHKRV IGQKEAVEVV ANAIRRSRAG LSDPNKPIGS
610 620 630 640 650
FLFLGPTGVG KTELCKTLAN FMFDSEDAMV RIDMSEFMEK HSVARLVGAP
660 670 680 690 700
PGYVGYEEGG YLTEAVRRKP YSVILLDEVE KAHPDVFNIL LQVLDDGRLT
710 720 730 740 750
DGQGRTVDFR NTVVIMTSNL GSSRIQENFA MLDYQGIKEQ VMEVVTKHFR
760 770 780 790 800
PEFLNRVDET VVFHPLSQDH IKSIAAIQLN RLANRMEEHG YQLEVSDKAL
810 820 830 840 850
ELIAQVGFDP VYGARPLKRA IQQSIENPLA KSILAGSVLP DKKIQLIVNN

DQIVAHQ
Length:857
Mass (Da):96,097
Last modified:December 15, 2003 - v1
Checksum:iF0FD9678638F8CFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000037 Genomic DNA. Translation: BAC93479.1.
RefSeqiWP_011149573.1. NC_005139.1.

Genome annotation databases

EnsemblBacteriaiBAC93479; BAC93479; BAC93479.
GeneIDi2623484.
KEGGivvy:VV0715.
PATRICi20168228. VBIVibVul40472_0734.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000037 Genomic DNA. Translation: BAC93479.1.
RefSeqiWP_011149573.1. NC_005139.1.

3D structure databases

ProteinModelPortaliQ7MNK1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC93479; BAC93479; BAC93479.
GeneIDi2623484.
KEGGivvy:VV0715.
PATRICi20168228. VBIVibVul40472_0734.

Phylogenomic databases

HOGENOMiHOG000218211.
KOiK03695.
OMAiIMVEPTS.
OrthoDBiPOG091H019M.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR017730. Chaperonin_ClpB.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR03346. chaperone_ClpB. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLPB_VIBVY
AccessioniPrimary (citable) accession number: Q7MNK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: December 15, 2003
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.