Reviewed, UniProtKB/Swiss-Prot Q7MN70 (PUR4_VIBVY)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	VV0847

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	1297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm. HAMAP MF_00419
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1297	1297	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000100423
Regions
	Domain	1044 – 1297	254	Glutamine amidotransferase type-1
	Nucleotide binding	307 – 318	12	ATP Potential
Sites
	Active site	1137	1	Nucleophile By similarity
	Active site	1262	1	By similarity
	Active site	1264	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MN70-1 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: DC9035912AD88570	FASTA	1,297	140,905
	10 20 30 40 50 60 MRILRGSPAL SEFRVNKLLE LCREQDLPVT GIYAEFMHFA DLTSELDAEA LEKLEKLLTY 70 80 90 100 110 120 GPTIEEHEPQ GLLLLVTPRP GTISPWSSKA TDIAQNCGLN AVKRLERGTA YYVESSSELS 130 140 150 160 170 180 SVQIDIVKSI IHDRMMEAVF GDLEAAAALF SVAQPAPMTQ VDILSGGRLA LEEANVSLGL 190 200 210 220 230 240 ALAEDEIDYL VENFTKLGRN PNDIELMMFA QANSEHCRHK IFNADWTIDG VEQPKSLFKM 250 260 270 280 290 300 IKNTFETTPD HVLSAYKDNA AVMTGSKVGR FFPDPETRQY NYHHEDAHIL MKVETHNHPT 310 320 330 340 350 360 AISPWPGAST GSGGEIRDEG ATGIGGKPKA GLVGFTTSNL RIPGFEQPWE TDFGKPGRIV 370 380 390 400 410 420 NALDIMLEGP LGGAAFNNEF GRPNLLGYFR TYEEKVTSHA GEEVRGYHKP IMIAGGMGNI 430 440 450 460 470 480 RDEHVQKKEI PVGASLIVLG GPAMNIGLGG GAASSMASGQ SAEDLDFASV QRENPEMERR 490 500 510 520 530 540 CQEVIDRCWQ LGDNNPIAFI HDVGAGGISN ALPELVNDGE RGGKFQLRDV PNDEPGMSPL 550 560 570 580 590 600 EIWCNESQER YVLAVAPENM AAFDAICKRE RAPYAVVGVA TEERHLTLED AHFDNTPIDM 610 620 630 640 650 660 PMDILLGKPP KMHREATTLK VDSPAMTRDG IELNEAVDRV LRLPTVAEKT FLITIGDRTV 670 680 690 700 710 720 TGLVARDQMV GPWQVPVANC AVTAASYDTY HGEAMSMGER TPVALLDFGA SARLAVGESL 730 740 750 760 770 780 TNIAATDIGD IKRIKLSANW MSPAGHPGED AGLYEAVKAV GEELCPALGL TIPVGKDSMS 790 800 810 820 830 840 MKTKWNENGE EKEVTSPLSL IITAFARVED VRKTITPQLR TDKGETSLVL VDLGNGKNRL 850 860 870 880 890 900 GATALAQVYK QLGDKPADVD NAEQLKGFFD AMQALVRQDK LLAYHDKGDG GLLVTLAEMA 910 920 930 940 950 960 FAGHCGVNAN IAALGDDVLA ALFNEELGAV VQVKNDELDS VLSTLAANGL EACSHVIGAI 970 980 990 1000 1010 1020 DASDNFVIRS GDVVVLERSR TDLRVIWAET THKMQALRDN PACADQEFEA KKDNSDPGLN 1030 1040 1050 1060 1070 1080 VSLSYEVNED IAAPYIAKGA KPKMAILREQ GVNSHVEMAA AFDRAGFEAT DIHMSDILTG 1090 1100 1110 1120 1130 1140 QAVLDEYHGL VACGGFSYGD VLGAGEGWAK SVLFNAQARE QFQAFFNREN TFSLGVCNGC 1150 1160 1170 1180 1190 1200 QMLSNLKELI PGADLWPRFV RNESERFEAR FSLVEVQKSD SVFFDGMAGS RMPIAVSHGE 1210 1220 1230 1240 1250 1260 GRVEVRDAQH LAAIEASGTV AVRFVDNLGN PTQQYPNNPN GSPNAITGLT TKDGRVTIMM 1270 1280 1290 PHPERVFRTV ANSWAPEGWG ENGAWMRMFQ NARKNLA

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000037 Genomic DNA. Translation: BAC93611.1. Different initiation.
RefSeq	NP_933640.1.
3D structure databases
SMR	Q7MN70. Positions 1-1297.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7MN70.
Genome annotation databases
GeneID	2623625.
GenomeReviews	Gene locus VV0847 in contig BA000037_GR.
KEGG	vvy:VV0847.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7MN70.
OMA	ERGIAYY.
Enzyme and pathway databases
BioCyc	VVUL196600:VV0847-MON.
Family and domain databases
HAMAP	MF_00419. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_VIBVY

Accession

Primary (citable) accession number: Q7MN70

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)