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Reviewed, UniProtKB/Swiss-Prot Q7MM23 (NRFA_VIBVY)

Last modified June 16, 2009. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase
Gene names
Name: nrfA
Ordered Locus Names: VV1250
OrganismVibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]
Taxonomic identifier196600 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

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Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Plays a role in nitrite reduction By similarity.

Catalytic activity

NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182

Cofactor

Binds 1 calcium ion per monomer By similarity.

Binds 5 heme groups covalently per monomer By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the cytochrome c-552 family.

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Ontologies

Keywords
   Biological processElectron transport
Transport
   Cellular componentPeriplasm
   DomainSignal
   LigandCalcium
Heme
Iron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processelectron transport chain

Inferred from electronic annotation. Source: UniProtKB-KW

nitrogen compound metabolic process

Inferred from electronic annotation. Source: HAMAP

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

heme binding

Inferred from electronic annotation. Source: InterPro

nitrite reductase (cytochrome, ammonia-forming) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 474445Cytochrome c-552 HAMAP MF_01182
PRO_0000268984

Sites

Metal binding911Iron (heme 3 axial ligand) By similarity
Metal binding1231Iron (heme 1 axial ligand) By similarity
Metal binding1611Iron (heme 2 axial ligand) By similarity
Metal binding2101Iron (heme 3 axial ligand) By similarity
Metal binding2121Calcium By similarity
Metal binding2131Calcium; via carbonyl oxygen By similarity
Metal binding2581Calcium; via carbonyl oxygen By similarity
Metal binding2601Calcium By similarity
Metal binding2721Iron (heme 5 axial ligand) By similarity
Metal binding2831Iron (heme 4 axial ligand) By similarity
Metal binding2981Iron (heme 2 axial ligand) By similarity
Metal binding3151Iron (heme 5 axial ligand) By similarity
Metal binding3901Iron (heme 4 axial ligand) By similarity
Binding site1191Heme 1 (covalent) By similarity
Binding site1221Heme 1 (covalent) By similarity
Binding site1571Heme 2 (covalent) By similarity
Binding site1601Heme 2 (covalent) By similarity
Binding site2061Heme 3 (covalent) By similarity
Binding site2091Heme 3 (covalent) By similarity
Binding site2131Substrate By similarity
Binding site2611Substrate By similarity
Binding site2791Heme 4 (covalent) By similarity
Binding site2821Heme 4 (covalent) By similarity
Binding site3111Heme 5 (covalent) By similarity
Binding site3141Heme 5 (covalent) By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q7MM23-1 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: AE11CC4539C1B8A4

FASTA47453,092
        10         20         30         40         50         60 
MSIKHWMASS VSVTALVMTA LLNITAVSAE EKGLVDPRND AFEQNHPDQY QSWKKTSESV 

        70         80         90        100        110        120 
EIEDALAEDP NMVILWAGYG FAKDYNKARG HFYALDDVRQ TLRTGGPQDA KSGPMPMACW 

       130        140        150        160        170        180 
SCKSPDVARV IDERGEDGYF EGKWARLGAE IANPIGCADC HDTRSEKFKN GEPELALTRP 

       190        200        210        220        230        240 
YVERAFQAIN KPFEQQSRLD KQASVCAQCH VEYYFTGPTK AVKFPWDMGT GVQQMEEYYD 

       250        260        270        280        290        300 
ALGFADWTHA VSKAPMLKAQ HPGFETWREG IHGKNKVVCV DCHMPKVKKE DGTVYTDHKV 

       310        320        330        340        350        360 
GNPFDRFEDT CAQCHTQSKD QLREIVSTRK AQVLNMKLTA EKQIVAAHFE AGAAWKAGAT 

       370        380        390        400        410        420 
EEEMKPILQD IRHAQWRWDY AIASHGVHMH APEVALEVLG TAVDRAADAR TKLVRLLATK 

       430        440        450        460        470 
GITEPVQIPD ISTKAAAQKA LGMDMEKMNA EKQHFLKTVV PDWDKAAAER ESKY

« Hide

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References

[1]"Comparative genome analysis of Vibrio vulnificus, a marine pathogen."
Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F.
Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

BA000037 Genomic DNA. Translation: BAC94014.1.
RefSeqNP_934043.1.

3D structure databases

HSSPHSSP built from PDB template 1GU6 based on UniProtKB P32050.
ModBaseSearch...

Genome annotation databases

GeneID2624054.
GenomeReviewsGene locus VV1250 in contig BA000037_GR.
KEGGvvy:VV1250.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ7MM23.
OMAQ7MM23. CHDQSKE.

Enzyme and pathway databases

BioCycVVUL196600:VV1250-MON.

Family and domain databases

HAMAPMF_01182.
[Tree]
InterProIPR003321. Cyt_c552.
IPR017570. Cyt_c_NO2Rdtase_formate-dep.
IPR011031. Multihaem_cyt.
[Graphical view]
PfamPF02335. Cytochrom_C552. 1 hit.
[Graphical view]
PIRSFPIRSF000243. Cyt_c552. 1 hit.
TIGRFAMsTIGR03152. cyto_c552_HCOOH. 1 hit.
PROSITEPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNRFA_VIBVY
AccessionPrimary (citable) accession number: Q7MM23
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 15, 2003
Last modified: June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents