Reviewed, UniProtKB/Swiss-Prot Q7MLY4 (DNAE2_VIBVY)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	VV1294

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	1024 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1024	1024	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103403

Sequences

	Sequence		Length	Mass (Da)
	Q7MLY4-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 79F35A55AE278D25	FASTA	1,024	116,479
	10 20 30 40 50 60 MFYAELFCQS NFSFLTGASH PEELIVQANF LGYHSLAITD ECSLAGIVRA HSAIKQHQLS 70 80 90 100 110 120 VKQIVGSFFK LNTECQFVLL CPNREAYAEL CRIITNARRR CEKGQYQLSQ WDVMSLKHCL 130 140 150 160 170 180 LIWLPTGQTS DEQWATWLSR YQQSRLWLGL QRHLHHDDEH YLHHCQTLAN QFDLPITACG 190 200 210 220 230 240 GVLMHRPERL PLQHTLTAIR HGCTVEQLGT QLLTNAEQSL RSEKKLRKLF KPQWLAESLY 250 260 270 280 290 300 IASLCSFDLD SLRYEYPSEL IPDGYTPDSY LEHLVEQGKK LRFPEGVPDA IEQTIQKELA 310 320 330 340 350 360 LIKEQKYPFF FLTIHDIVMF AKQQGILYQG RGSAANSVVC YCLQITSVDP RQIAVLFERF 370 380 390 400 410 420 ISKERDEPPD IDVDFEHERR EEVIQYIYQK YGRQRAALAA TVISYRFKSA VRQVGKALGI 430 440 450 460 470 480 EETQLDFFLK NINRRDRQAG WQVQLVELGL RPDSLKGQHF IQLVEEIIGF PRHLSQHVGG 490 500 510 520 530 540 FVISSGPLYE LVPVENAAMP ERTIIQWDKD DLESLKLLKV DVLSLGMLTA IRKCFRSIEQ 550 560 570 580 590 600 HHQQRLSIAD ITRRQDDQAV YKMIQKADTI GVFQIESRAQ MSMLPRLKPA CYYDLVIQIA 610 620 630 640 650 660 IVRPGPIQGD MVHPFLKRRD GEEPISYPSV HVQEVLERTL GVPIFQEQVI KLAMVAAGFS 670 680 690 700 710 720 GGEADQLRRA MAAWKKNGHV FKFKTKLING MLERGYELDF AERIFEQICG FGEYGFPESH 730 740 750 760 770 780 SASFAVLAYC SAWLKHYYPA EFYTALLNSQ PMGFYSPSQL VQDAKRHGIA VLPICVNFSN 790 800 810 820 830 840 PEHQLVRLTS GELAIRLGFN LIKGLSHEGI TRLLTHRPAQ GYQCISEVKQ ILRQAKDIQS 850 860 870 880 890 900 LASANAFYQL ADNRYLARWQ VMDNLDELPL FQTLPSISNN PLPKPSDYQN VLEDYAATGL 910 920 930 940 950 960 SLAEHPVAML EKAGGLTRFT RANQLNQCSH RSLVTVIGLV TGKQSPGTAA GVTFFTLEDD 970 980 990 1000 1010 1020 TGNINVVVWQ ATSRAQKQAY LTARLLMVKG ILEREGDVIH VIAGRLVDLT EKLSGLSPKS REFH

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000037 Genomic DNA. Translation: BAC94056.1.
RefSeq	NP_934085.1.
3D structure databases
SMR	Q7MLY4. Positions 3-867.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7MLY4.
Genome annotation databases
GeneID	2624096.
GenomeReviews	Gene locus VV1294 in contig BA000037_GR.
KEGG	vvy:VV1294.
NMPDR	fig|196600.1.peg.1360.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	MGFYSPD.
Enzyme and pathway databases
BioCyc	VVUL196600:VV1294-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_VIBVY

Accession

Primary (citable) accession number: Q7MLY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	December 15, 2003
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)