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Q7MLU7 (BIOD_VIBVY) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3
Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase
Gene names
Name:bioD
Ordered Locus Names:VV1330
OrganismVibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]
Taxonomic identifier196600 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336

Catalytic activity

ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336

Cofactor

Magnesium By similarity. HAMAP MF_00336

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336

Subcellular location

Cytoplasm By similarity HAMAP MF_00336.

Sequence similarities

Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

dethiobiotin synthase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336
PRO_0000187996

Regions

Nucleotide binding13 – 186ATP By similarity
Nucleotide binding116 – 1194ATP By similarity
Nucleotide binding176 – 1772ATP By similarity
Nucleotide binding205 – 2073ATP By similarity

Sites

Metal binding131Magnesium 1 By similarity
Metal binding171Magnesium 2 By similarity
Metal binding551Magnesium 2 By similarity
Metal binding1161Magnesium 2 By similarity
Binding site551ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7MLU7 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 629A4A84ED74BFFD

FASTA22724,636
        10         20         30         40         50         60 
MMKAFFIAGT DTDVGKTVAS KAILHALAAK DLRTIGYKPV AAGSDKTPEG WRNSDALHLQ 

        70         80         90        100        110        120 
KAATVAVDYE DINPYALELP ASPHIAAKHE KVEIDYAVLS RKLAQHKENA DVVLVEGAGG 

       130        140        150        160        170        180 
WRVPVSDTDS LSTWVKQEDL PVVLVVGIKL GCLSHALLTA EVIKADGLNL VGWVANRVNP 

       190        200        210        220 
GTEHYAEIID MLEERLEVPK LGEIPYIPSA KRKDLGKYIN ADVLLEL

« Hide

References

[1]"Comparative genome analysis of Vibrio vulnificus, a marine pathogen."
Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F.
Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: YJ016.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000037 Genomic DNA. Translation: BAC94094.1.
RefSeqNP_934123.1. NC_005139.1.

3D structure databases

ProteinModelPortalQ7MLU7.
SMRQ7MLU7. Positions 3-224.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ7MLU7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2624134.
GenomeReviewsGene locus VV1330 in contig BA000037_GR.
KEGGvvy:VV1330.
PATRIC20169466. VBIVibVul40472_1320.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0132.
HOGENOMHBG650065.
OMAALAAYWQ.
ProtClustDBPRK00090.

Enzyme and pathway databases

BioCycVVUL196600:VV1330-MONOMER.

Family and domain databases

HAMAPMF_00336. BioD.
[Tree]
InterProIPR002586. CbiA_P_synth.
IPR004472. DTB_synth_BioD.
[Graphical view]
KOK01935.
PfamPF01656. CbiA. 1 hit.
[Graphical view]
PIRSFPIRSF006755. DTB_synth. 1 hit.
TIGRFAMsTIGR00347. BioD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOD_VIBVY
AccessionPrimary (citable) accession number: Q7MLU7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: December 15, 2003
Last modified: January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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