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Protein

Histidine biosynthesis bifunctional protein HisIE

Gene

hisI

Organism
Vibrio vulnificus (strain YJ016)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate.UniRule annotation
1-(5-phospho-beta-D-ribosyl)-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide.UniRule annotation

Pathwayi: L-histidine biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG)
  2. Histidine biosynthesis bifunctional protein HisIE (hisI)
  3. Histidine biosynthesis bifunctional protein HisIE (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit hisF1 (hisF1), Imidazole glycerol phosphate synthase subunit HisH 3 (hisH3), Imidazole glycerol phosphate synthase subunit HisH 1 (hisH1), Putative imidazole glycerol phosphate synthase subunit hisF3 (hisF3), Imidazole glycerol phosphate synthase subunit HisH 2 (hisH2), Putative imidazole glycerol phosphate synthase subunit hisF2 (hisF2)
  6. Histidine biosynthesis bifunctional protein HisB (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. Histidine biosynthesis bifunctional protein HisB (hisB)
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciVVUL196600:GJ9W-1412-MONOMER.
UniPathwayiUPA00031; UER00007.
UPA00031; UER00008.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine biosynthesis bifunctional protein HisIEUniRule annotation
Including the following 2 domains:
Phosphoribosyl-AMP cyclohydrolaseUniRule annotation (EC:3.5.4.19UniRule annotation)
Short name:
PRA-CHUniRule annotation
Phosphoribosyl-ATP pyrophosphataseUniRule annotation (EC:3.6.1.31UniRule annotation)
Short name:
PRA-PHUniRule annotation
Gene namesi
Name:hisIUniRule annotation
Synonyms:hisIEUniRule annotation
Ordered Locus Names:VV1357
OrganismiVibrio vulnificus (strain YJ016)
Taxonomic identifieri196600 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002675 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Histidine biosynthesis bifunctional protein HisIEPRO_0000136448Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ7MLS0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 122122Phosphoribosyl-AMP cyclohydrolaseAdd
BLAST
Regioni123 – 21189Phosphoribosyl-ATP pyrophosphohydrolaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the PRA-CH family.UniRule annotation
In the C-terminal section; belongs to the PRA-PH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000277503.
KOiK11755.
OMAiERSCFHQ.
OrthoDBiEOG6PGKB6.

Family and domain databases

HAMAPiMF_01019. HisIE.
MF_01020. HisE.
InterProiIPR023019. His_synth_HisIE.
IPR008179. PRib-ATP_PPHydrolase.
IPR021130. PRib-ATP_PPHydrolase-like.
IPR002496. PRib_AMP_CycHydrolase_dom.
[Graphical view]
PfamiPF01502. PRA-CH. 1 hit.
PF01503. PRA-PH. 1 hit.
[Graphical view]
ProDomiPD002610. PRA_CycHdrlase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03188. histidine_hisI. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7MLS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVKAAEVSS LAERINWEKV DGLVPAIVQD FQSSQVLMMG YMNQDALAKT
60 70 80 90 100
GETGQVTFFS RTKERLWTKG ETSGNVLQLV NMSLDCDNDT LLVKVNPIGP
110 120 130 140 150
TCHTGTTTCW DGDPQEESQM VWLHQLEQLL AARKSADPDS SYTASLYARG
160 170 180 190 200
TKRISQKVGE EGVEVALAAT SGDKAELVCE SADLIYHLLV LLQDQGLSMN
210
DVINKLKERH K
Length:211
Mass (Da):23,220
Last modified:December 15, 2003 - v1
Checksum:iA6440E671C35469B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000037 Genomic DNA. Translation: BAC94121.1.
RefSeqiWP_011150024.1. NC_005139.1.

Genome annotation databases

EnsemblBacteriaiBAC94121; BAC94121; BAC94121.
GeneIDi2624161.
KEGGivvy:VV1357.
PATRICi20169518. VBIVibVul40472_1346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000037 Genomic DNA. Translation: BAC94121.1.
RefSeqiWP_011150024.1. NC_005139.1.

3D structure databases

ProteinModelPortaliQ7MLS0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC94121; BAC94121; BAC94121.
GeneIDi2624161.
KEGGivvy:VV1357.
PATRICi20169518. VBIVibVul40472_1346.

Phylogenomic databases

HOGENOMiHOG000277503.
KOiK11755.
OMAiERSCFHQ.
OrthoDBiEOG6PGKB6.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00007.
UPA00031; UER00008.
BioCyciVVUL196600:GJ9W-1412-MONOMER.

Family and domain databases

HAMAPiMF_01019. HisIE.
MF_01020. HisE.
InterProiIPR023019. His_synth_HisIE.
IPR008179. PRib-ATP_PPHydrolase.
IPR021130. PRib-ATP_PPHydrolase-like.
IPR002496. PRib_AMP_CycHydrolase_dom.
[Graphical view]
PfamiPF01502. PRA-CH. 1 hit.
PF01503. PRA-PH. 1 hit.
[Graphical view]
ProDomiPD002610. PRA_CycHdrlase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03188. histidine_hisI. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YJ016.

Entry informationi

Entry nameiHIS2_VIBVY
AccessioniPrimary (citable) accession number: Q7MLS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: December 15, 2003
Last modified: November 11, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.