Reviewed, UniProtKB/Swiss-Prot Q7MLQ2 (TORA_VIBVY)

Names and origin

Protein names

Recommended name:
    Trimethylamine-N-oxide reductase
      Short name=TMAO reductase
      Short name=Trimethylamine oxidase
    EC=1.7.2.3

Gene names

Name:	torA
Ordered Locus Names:	VV1375

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	820 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions By similarity.
Catalytic activity	Trimethylamine + 2 (ferricytochrome c)-subunit + H2O = trimethylamine N-oxide + 2 (ferrocytochrome c)-subunit + 2 H+.
Cofactor	Molybdenum (molybdopterin) By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW trimethylamine-N-oxide reductase (cytochrome c) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Tat-type signal Potential
	Chain	34 – 820	787	Trimethylamine-N-oxide reductase		PRO_0000019162

Sequences

	Sequence		Length	Mass (Da)
	Q7MLQ2-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 763E05D67D3DB90C	FASTA	820	92,469
	10 20 30 40 50 60 MAITRRSFLK GVATTSAASV IGPSLLASAS ANAVETTGTW KVSGSHWGAF RAHIYAGKVQ 70 80 90 100 110 120 EIKPIELDQN PTEMLNGIKG IIYSPSRVRY PMVRLDWLKK HKYSADTRGN NRFVRVTWDE 130 140 150 160 170 180 ALDLFYRELE RVQKEYGPWA LHAGQTGWNQ TGSFNNCTAH MQRAVGMHGN YITKVGDYST 190 200 210 220 230 240 GAGQTILPYV LGSTEVYAQG TSWSEILENA DNIILWANDP VKNLQVGWNC ETHESYAYLA 250 260 270 280 290 300 QLKEKVAKGE INVISVDPVK NKTQRYLEND HLYVNPMTDV PFMLAIAHVL YTENLYDKKF 310 320 330 340 350 360 IETYCLGFEE FINYVQGKTK DKVEKTPEWA APICGVKADK IREFARMLVK GRTQILMGWC 370 380 390 400 410 420 IQRQEHGEQP YWAAAVVAAM IGQIGLPGGG ISYGHHYSSI GVPSTGFAGP GGFPRNLDAG 430 440 450 460 470 480 MKPKWDNNDF NGYSRTIPVA RWIDCLLEPG KEINYNGGKV KLPDFKMMVI SGCNPWHHHQ 490 500 510 520 530 540 DRNRMKQAFQ KLQTVVTIDF AWTATCRFSD IVLPACTQWE RNDIDVYGSY SSRGLIAMHR 550 560 570 580 590 600 LVDPLFQSKP DFQIMKELTE RFGRSEEYSR GMSEMDWIRS LYNDCKKSNE GKFEMPEFDE 610 620 630 640 650 660 FWEKSVLDFG QGQPWVRHAD FRQDPEINPL GTPSGFIEIT SRKIGRYGYE HCQEHPMWFE 670 680 690 700 710 720 KSERSHGGPG SDKHPFWLQS CHPDKRLHSQ MCESEEFRAT YAVKGREPVY INPLDAKAKG 730 740 750 760 770 780 IKEGDLVRVF NDRGQLLAGA VLTDSYPRGV IRIEEGAWYG PLSEKVGAIC TYGDPNTLTQ 790 800 810 820 DIGSSELAQA TSANTCIVDF EKFTGKVPPV TSFGGPIEVA

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000037 Genomic DNA. Translation: BAC94139.1.
RefSeq	NP_934168.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7MLQ2.
Genome annotation databases
GeneID	2624179.
GenomeReviews	Gene locus VV1375 in contig BA000037_GR.
KEGG	vvy:VV1375.
NMPDR	fig|196600.1.peg.1443.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7MLQ2.
OMA	NAETHES.
Enzyme and pathway databases
BioCyc	VVUL196600:VV1375-MON.
Family and domain databases
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR006311. TAT_signal. IPR011887. TorA. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit. TIGR02164. torA. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. False negative. PS00490. MOLYBDOPTERIN_PROK_2. 1 hit. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TORA_VIBVY

Accession

Primary (citable) accession number: Q7MLQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	December 15, 2003
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)