Reviewed, UniProtKB/Swiss-Prot Q7MLG3 (MAO11_VIBVY)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme 1
      Short name=NAD-ME 1
    EC=1.1.1.38

Gene names

Name:	sfcA1
Ordered Locus Names:	VV1464

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	NAD-dependent malic enzyme 1		PRO_0000160238
Sites
	Active site	101	1	Proton donor By similarity
	Active site	172	1	Proton acceptor By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Binding site	154	1	NAD By similarity
	Binding site	267	1	NAD By similarity
	Binding site	415	1	NAD By similarity
	Site	267	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MLG3-1 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: F05F1BC579ABBAB1	FASTA	562	62,088
	10 20 30 40 50 60 MNNDKRPLYI PFAGPALLST PLLNKGSAFS AEERISFNLE GLLPETTETI QEQVERAYMQ 70 80 90 100 110 120 YKAFESDMDK HIYLRNIQDT NETLFYRLVQ NHITEMMPII YTPTVGAACE NFSNIYRRGR 130 140 150 160 170 180 GLFVSYANRD RIDDILNNAS NHNVKVIVVT DGERILGLGD QGIGGMGIPI GKLSLYTACG 190 200 210 220 230 240 GISPAYTLPI VLDVGTNNPQ RLADPMYMGW RHPRITGADY DAFVEEFIQA VQRRWPDALI 250 260 270 280 290 300 QFEDFAQKNA MPLLERYKNR ICCFNDDIQG TAAVTVGSLM AACQAAGSKL SEQRITFLGA 310 320 330 340 350 360 GSAGCGIAEA IIAQMVSEGI SDKKARSQVF MVDRWGLLQE GMPNLLDFQQ RLVQKHSVTA 370 380 390 400 410 420 KWETEANGFS LLDVVKNAKP TVLIGVSGAP GLFTQEVIQE MHKHCPRPIV FPLSNPTSRV 430 440 450 460 470 480 EAVPADIIRW TNGDALVATG SPFDPVIHEG KTYPIVQCNN SYIFPGIGLG VLAVNAKRVT 490 500 510 520 530 540 DEMLMESSRA LATCSPLAIN GKGALLPPLE EIHTVSKRIA YAVAKKAIEQ GVALEIADDA 550 560 LQVAIEQHFW QPVYRRYKRT AF

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000037 Genomic DNA. Translation: BAC94228.1. Different initiation.
RefSeq	NP_934257.1.
3D structure databases
HSSP	HSSP built from PDB template 1GQ2 based on UniProtKB P40927.
ModBase	Search...
Genome annotation databases
GeneID	2624268.
GenomeReviews	Gene locus VV1464 in contig BA000037_GR.
KEGG	vvy:VV1464.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7MLG3.
Enzyme and pathway databases
BioCyc	VVUL196600:VV1464-MON.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO11_VIBVY

Accession

Primary (citable) accession number: Q7MLG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	November 25, 2008
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)