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Protein

Ribosomal RNA small subunit methyltransferase F

Gene

rsmF

Organism
Vibrio vulnificus (strain YJ016)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei147 – 1471S-adenosyl-L-methionineUniRule annotation
Binding sitei174 – 1741S-adenosyl-L-methionineUniRule annotation
Binding sitei192 – 1921S-adenosyl-L-methionineUniRule annotation
Active sitei245 – 2451NucleophileUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciVVUL196600:GJ9W-1698-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase FUniRule annotation (EC:2.1.1.178UniRule annotation)
Alternative name(s):
16S rRNA m5C1407 methyltransferaseUniRule annotation
rRNA (cytosine-C(5)-)-methyltransferase RsmFUniRule annotation
Gene namesi
Name:rsmFUniRule annotation
Ordered Locus Names:VV1641
OrganismiVibrio vulnificus (strain YJ016)
Taxonomic identifieri196600 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002675 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472Ribosomal RNA small subunit methyltransferase FPRO_0000285026Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ7MKY6.
SMRiQ7MKY6. Positions 6-471.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1297S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218115.
KOiK11392.
OMAiWCAEGFW.
OrthoDBiPOG091H02E2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01579. 16SrRNA_methyltr_F. 1 hit.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR027391. Nol1_Nop2_Fmu_2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF13636. Methyltranf_PUA. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7MKY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSNVYLPET FLEKIKTILP SALNMDDFIA YCQQPLRKSI RVNTLKISVD
60 70 80 90 100
AFLTIAQEKG WTLHPVPWCE TGFWIEADES KVPLGNTAEH MCGLFYIQEA
110 120 130 140 150
SSMMPVSALF LGENQFESVL DTAAAPGSKT TQIAALMNNQ GILVANEYAA
160 170 180 190 200
SRVKVLHANI ERCGVRNAAL SNFDGRVFGG WLPESFDAVL LDAPCSGEGT
210 220 230 240 250
VRKDEDAMKN WSQESVLEIA ATQKDLIESA FQALKPGGVM VYSTCTLSEE
260 270 280 290 300
ENQQVCHHLK HTFGDAVAFE SLSSLFDGAE KALTEDGFLH IFPQVYDSEG
310 320 330 340 350
FFVARIRKLA SVNSPDVNKK MGKFPFQKAS KKESALIEKS LSDSLDISLP
360 370 380 390 400
ADSTIWLRDN DVWLFPNALE PMLGELRFSR MGIKLAEKHK NGYRWQHQIA
410 420 430 440 450
TTLASGDEKT VVDIDTDAAR EWFMGRDIYP ENSGKGEVFV RYQGAIIGLG
460 470
KWVSNKIKNG LPRELVRDKN LF
Length:472
Mass (Da):52,548
Last modified:May 1, 2007 - v2
Checksum:i26EA702191D33857
GO

Sequence cautioni

The sequence BAC94405 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000037 Genomic DNA. Translation: BAC94405.1. Different initiation.
RefSeqiWP_011080492.1. NC_005139.1.

Genome annotation databases

EnsemblBacteriaiBAC94405; BAC94405; BAC94405.
GeneIDi2624447.
KEGGivvy:VV1641.
PATRICi20170070. VBIVibVul40472_1620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000037 Genomic DNA. Translation: BAC94405.1. Different initiation.
RefSeqiWP_011080492.1. NC_005139.1.

3D structure databases

ProteinModelPortaliQ7MKY6.
SMRiQ7MKY6. Positions 6-471.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC94405; BAC94405; BAC94405.
GeneIDi2624447.
KEGGivvy:VV1641.
PATRICi20170070. VBIVibVul40472_1620.

Phylogenomic databases

HOGENOMiHOG000218115.
KOiK11392.
OMAiWCAEGFW.
OrthoDBiPOG091H02E2.

Enzyme and pathway databases

BioCyciVVUL196600:GJ9W-1698-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01579. 16SrRNA_methyltr_F. 1 hit.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR027391. Nol1_Nop2_Fmu_2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF13636. Methyltranf_PUA. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSMF_VIBVY
AccessioniPrimary (citable) accession number: Q7MKY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: September 7, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.