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Q7MKX3 (PYRD_VIBVY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydroorotate dehydrogenase (quinone)

EC=1.3.5.2
Alternative name(s):
DHOdehase
Short name=DHOD
Short name=DHODase
Dihydroorotate oxidase
Gene names
Name:pyrD
Ordered Locus Names:VV1654
OrganismVibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]
Taxonomic identifier196600 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor By similarity. HAMAP-Rule MF_00225

Catalytic activity

(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP-Rule MF_00225

Cofactor

Binds 1 FMN per subunit By similarity. HAMAP-Rule MF_00225

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP-Rule MF_00225

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00225

Subcellular location

Cell membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_00225.

Sequence similarities

Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   Cellular componentCell membrane
Membrane
   LigandFlavoprotein
FMN
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_process'de novo' UMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

'de novo' pyrimidine nucleobase biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondihydroorotate oxidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336Dihydroorotate dehydrogenase (quinone) HAMAP-Rule MF_00225
PRO_0000148488

Regions

Nucleotide binding62 – 665FMN By similarity
Nucleotide binding318 – 3192FMN By similarity
Region111 – 1155Substrate binding By similarity
Region246 – 2472Substrate binding By similarity

Sites

Active site1751Nucleophile By similarity
Binding site661Substrate By similarity
Binding site861FMN; via amide nitrogen By similarity
Binding site1391FMN By similarity
Binding site1721FMN By similarity
Binding site1721Substrate By similarity
Binding site1771Substrate By similarity
Binding site2171FMN By similarity
Binding site2451FMN; via carbonyl oxygen By similarity
Binding site2681FMN; via amide nitrogen By similarity
Binding site2971FMN; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7MKX3 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 8B105A8C1F309AEF

FASTA33636,979
        10         20         30         40         50         60 
MLYRLARAGF FQLDAEKAHD LAIQNFKRFT GTPIDLFYRQ QLPNRPVECM GLTFRNPVGL 

        70         80         90        100        110        120 
AAGLDKNGEC IEAFDAMGFG FVEIGTVTPR AQSGNDKPRL FRLVGAEGII NRMGFNNLGV 

       130        140        150        160        170        180 
DNLIENVKKA KYSCVLGINI GKNKDTPIEK GAEDYLICME KVYEYAGYIA VNISSPNTPG 

       190        200        210        220        230        240 
LRTLQYGEAL DELLVELKRK QAELEEKHGK YVPLALKIAP DLTDDEISQI CQSLINNKID 

       250        260        270        280        290        300 
GVIATNTTLD RTMVEGMKYA QEAGGLSGRP LQSRSTEVVR LLRKELQGNI PIIGVGGVDS 

       310        320        330 
YVAAKEKMLA GADLVQVYSG FIYHGPGLVR DIVKNL 

« Hide

References

[1]"Comparative genome analysis of Vibrio vulnificus, a marine pathogen."
Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F.
Genome Res. 13:2577-2587(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: YJ016.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000037 Genomic DNA. Translation: BAC94418.1.
RefSeqNP_934447.1. NC_005139.1.

3D structure databases

ProteinModelPortalQ7MKX3.
SMRQ7MKX3. Positions 1-336.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196600.VV1654.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC94418; BAC94418; BAC94418.
GeneID2624460.
KEGGvvy:VV1654.
PATRIC20170094. VBIVibVul40472_1632.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0167.
HOGENOMHOG000225103.
KOK00254.
OMAGECIDAF.
OrthoDBEOG65BDN8.

Enzyme and pathway databases

BioCycVVUL196600:GJ9W-1711-MONOMER.
UniPathwayUPA00070; UER00946.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00225. DHO_dh_type2.
InterProIPR013785. Aldolase_TIM.
IPR012135. Dihydroorotate_DH_1_2.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
PfamPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsTIGR01036. pyrD_sub2. 1 hit.
PROSITEPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRD_VIBVY
AccessionPrimary (citable) accession number: Q7MKX3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: December 15, 2003
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways