Reviewed, UniProtKB/Swiss-Prot Q7MJC0 (MAO12_VIBVY)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme 2
      Short name=NAD-ME 2
    EC=1.1.1.38

Gene names

Name:	sfcA2
Ordered Locus Names:	VV2242

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	NAD-dependent malic enzyme 2 HAMAP MF_01619		PRO_0000160239
Sites
	Active site	98	1	Proton donor By similarity
	Active site	169	1	Proton acceptor By similarity
	Metal binding	240	1	Divalent metal cation By similarity
	Metal binding	241	1	Divalent metal cation By similarity
	Metal binding	264	1	Divalent metal cation By similarity
	Binding site	151	1	NAD By similarity
	Binding site	264	1	NAD By similarity
	Binding site	413	1	NAD By similarity
	Site	264	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MJC0-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 402FC1DAC6689471	FASTA	559	62,073
	10 20 30 40 50 60 MSKKMFIRQA GNTLLNTPLL NKGSAFTLEE RKNFNLIGLL PANIETIDEQ VSRAYEQFSL 70 80 90 100 110 120 FNSAMEKHIY LRNIQDTNET LYFRLINQHI EEMMPIIYTP TVGEACQKFS QIYRRNRGLF 130 140 150 160 170 180 LSFEDQDELE AILNNAPNTH VKVIVITDGE RILGLGDQGI GGMGIPIGKL ALYTACGGIS 190 200 210 220 230 240 PEHTLPIVLD VGTNNSALLS DPMYMGWRHP RITGDQYDDF VDDCLKAIRR RWPNALIQFE 250 260 270 280 290 300 DFAQANAMPL LMRYQNQFCC FNDDIQGTAS VTVGTLLAAA HATGKKLSAQ KVLFAGAGSA 310 320 330 340 350 360 GCGIAEAIVA QMVSEGISVQ QARSQVFMVD RWGMLEQEMP NLLPFQKPLA QPASLRTEWQ 370 380 390 400 410 420 IEANREISLL DVIQHAHPDV LIGVTGVPGL FNQEIIEAMA EDCERPVVMP LSNPTSRVEA 430 440 450 460 470 480 KPEDILMWTQ GQAIVATGSP FPDVVLAGKR YPIAQCNNSY IFPGVGLGVI SANAHRVTNE 490 500 510 520 530 540 MLQQASITLA SLSPMLNGGN MLLPPLSEIQ NVSRKIALEV AKKAVEQGKA SHRTEERLLE 550 RIDEEFWYAQ YCEYRRIAS

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000037 Genomic DNA. Translation: BAC95006.1.
RefSeq	NP_935035.1.
3D structure databases
HSSP	HSSP built from PDB template 1O0S based on UniProtKB P27443.
ModBase	Search...
Genome annotation databases
GeneID	2625048.
GenomeReviews	Gene locus VV2242 in contig BA000037_GR.
KEGG	vvy:VV2242.
NMPDR	fig|196600.1.peg.2310.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7MJC0.
OMA	Q7MJC0. PANIETI.
Enzyme and pathway databases
BioCyc	VVUL196600:VV2242-MON.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO12_VIBVY

Accession

Primary (citable) accession number: Q7MJC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)