Reviewed, UniProtKB/Swiss-Prot Q7MIQ5 (CHEB1_VIBVY)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	VV2460

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon HAMAP MF_00099		PRO_0000158041
Regions
	Domain	4 – 121	118	Response regulatory
	Domain	183 – 376	194	CheB-type methylesterase
Sites
	Active site	195	1	By similarity
	Active site	222	1	By similarity
	Active site	318	1	By similarity
Amino acid modifications
	Modified residue	55	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MIQ5-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: EA0A436C18A06F10	FASTA	376	40,488
	10 20 30 40 50 60 MAIKVLVVDD SSFFRRRVSE IINAESRLEV IDVAVNGKEA VEKAKRLKPD VITMDIEMPV 70 80 90 100 110 120 MDGISAVREI MASVPTPILM FSSLTHDGAK ATLDALDAGA LDFLPKKFED IARNRDEAVS 130 140 150 160 170 180 LLQQRVIQIA SKRAFMRRPV ASSTPVQERP QSTLNRPTTG LRREASAQAP VSRAPVAAKF 190 200 210 220 230 240 RASGKKYQLT AIGTSTGGPV ALQKILTKLP ANYPHPIVLI QHMPATFTAA FASRLNSLCK 250 260 270 280 290 300 IQVKEAEDGD VLQAGVAYLA PGGKQMMIDG RPGAARLRII DGGERMNYKP CVDVTFGSAA 310 320 330 340 350 360 KIYADKVLSM VLTGMGADGR EGARMLKSAG ATIWAQDEDS CVVYGMPQAV AKAGLSTEDL 370 PLERIAERML VEVGLA

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000037 Genomic DNA. Translation: BAC95224.1.
RefSeq	NP_935253.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7MIQ5.
Genome annotation databases
GeneID	2625268.
GenomeReviews	Gene locus VV2460 in contig BA000037_GR.
KEGG	vvy:VV2460.
NMPDR	fig|196600.1.peg.2528.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	IWAQDEA.
Enzyme and pathway databases
BioCyc	VVUL196600:VV2460-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_VIBVY

Accession

Primary (citable) accession number: Q7MIQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	December 15, 2003
Last modified:	January 19, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)