Reviewed, UniProtKB/Swiss-Prot Q7MII4 (GLO22_VIBVY)

Names and origin

Protein names

Recommended name:
    Hydroxyacylglutathione hydrolase 2
    EC=3.1.2.6
Alternative name(s):
    Glyoxalase II 2
      Short name=Glx II 2

Gene names

Name:	gloB2
Ordered Locus Names:	VV2532

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	Hydroxyacylglutathione hydrolase 2 HAMAP MF_01374		PRO_0000309723
Sites
	Metal binding	54	1	Zinc 1 By similarity
	Metal binding	56	1	Zinc 1 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	59	1	Zinc 2 By similarity
	Metal binding	111	1	Zinc 1 By similarity
	Metal binding	128	1	Zinc 1 By similarity
	Metal binding	128	1	Zinc 2 By similarity
	Metal binding	166	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MII4-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: A751AFD6CBB8F8D1	FASTA	252	28,248
	10 20 30 40 50 60 MLEIKSIPAF NDNYIWLIQN SDQRCAVVDP GDAKPVLHYI EQHQLTLEAI LITHHHNDHI 70 80 90 100 110 120 GGVADLVRAF PNVNVVGPKA EPIPTLTHPV EDGDRLELFD ETFLVLGLGG HTLGHIGYVG 130 140 150 160 170 180 DGKLFCGDVL FSAGCGRIFE GTAQQMFDSL NKLLALPEET EVFCAHEYTA SNVAFALAVE 190 200 210 220 230 240 PDNELLHQYR DTVNRLRAQN LPTIPTTLRQ EKWINPFLRY LQPSVIHSVS SRTKNSDPLS 250 VFTALREWKN EF

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000037 Genomic DNA. Translation: BAC95296.1.
RefSeq	NP_935325.1.
3D structure databases
HSSP	HSSP built from PDB template 1XM8 based on UniProtKB Q9SID3.
SMR	Q7MII4. Positions 2-252.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7MII4.
Genome annotation databases
GeneID	2625340.
GenomeReviews	Gene locus VV2532 in contig BA000037_GR.
KEGG	vvy:VV2532.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	WCAHEYT.
Enzyme and pathway databases
BioCyc	VVUL196600:VV2532-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Blactmase-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO22_VIBVY

Accession

Primary (citable) accession number: Q7MII4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	December 15, 2003
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)