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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Vibrio vulnificus (strain YJ016)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciVVUL196600:GJ9W-2618-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:VV2559
OrganismiVibrio vulnificus (strain YJ016)
Taxonomic identifieri196600 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000002675 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 873873Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192775Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ7MIF8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini452 – 584133HDUniRule annotationAdd
BLAST
Domaini693 – 77381ACT 1UniRule annotationAdd
BLAST
Domaini800 – 87374ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 332332UridylyltransferaseAdd
BLAST
Regioni333 – 692360Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiSTIGERV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR010043. UTase/UR.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7MIF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFQSPLTFN DEQLTVAQLK SQLDLFANAQ KQAFLNHHPV TDLVLSRAEY
60 70 80 90 100
MDLLLTRLWR YYGFSEIHNI SLVAVGGYGR GELHPLSDID ILVLSKHKLP
110 120 130 140 150
GELETKLSEF ITLLWDLRLE VGHAVRTVEE CAAIGREDLT VATNLQEARL
160 170 180 190 200
LCGSENTFQD LKKVVLSDSF WPSETFYRAK IQEQRERHAR YHDTTYNLEP
210 220 230 240 250
DIKSTPGGLR DIHTLSWVAR RHFGATSLLE MSRYGFLTDA EYRELVECQD
260 270 280 290 300
FLWRVRFALH IELKRYDNRL TFAHQIQVAE HLGFKGEGNR GIEMMMKEFY
310 320 330 340 350
RTLRRVAELN KMLLKLFDQA IINGGETEPA VIINEDFQRR GRLIEARKPA
360 370 380 390 400
LFQARPETIL DMFLHIANDS TIDSVSPPTL RQLRTARRRL NKFLHTIPEA
410 420 430 440 450
REKFMELVRH PNALHRAFSL MHKLGVLAAY LPQWSQIVGQ MQFDLFHVYT
460 470 480 490 500
VDEHSVRLLN HINTFSYAKN HDKHPICCEV YPRLQKKELL LLAAIFHDIG
510 520 530 540 550
KGRGGDHSEI GEKEAYDFCI EHGLSKPEAK LVSWLVRHHL LMSVTAQRRD
560 570 580 590 600
IYDPEVITEF AKQVRDEERL EYLVCLTVAD ICATNPELWN SWKRTLLAEL
610 620 630 640 650
FYSTQRALRR GLENPVDVRE RIRHNQQLAS ALLRKEGFTA REIEVLWQRF
660 670 680 690 700
KADYFLRHTH KQIAWHCEHI LRMDNPEQPL VLMSKKATRG GTEVFVYTKD
710 720 730 740 750
QHALFATVVA ELDRRNFNVH DAQIMSSKDG YVLDTFMVLD QHGQAIDVDN
760 770 780 790 800
HKAVIKHLMH VLTDGRPTKV KTRRTPYKLQ HFKVKTKVDF LPTKSKKRTL
810 820 830 840 850
MELVALDTPG LLAITGATFA DMGFNLHGAK ITTIGERAED LFILTSENGG
860 870
RLSEEQELQL REKLIHNIAE LAP
Length:873
Mass (Da):101,060
Last modified:December 15, 2003 - v1
Checksum:iEF8625319678607A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000037 Genomic DNA. Translation: BAC95323.1.
RefSeqiNP_935352.1. NC_005139.1.
WP_011150965.1. NC_005139.1.

Genome annotation databases

EnsemblBacteriaiBAC95323; BAC95323; BAC95323.
GeneIDi2625367.
KEGGivvy:VV2559.
PATRICi20171960. VBIVibVul40472_2563.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000037 Genomic DNA. Translation: BAC95323.1.
RefSeqiNP_935352.1. NC_005139.1.
WP_011150965.1. NC_005139.1.

3D structure databases

ProteinModelPortaliQ7MIF8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC95323; BAC95323; BAC95323.
GeneIDi2625367.
KEGGivvy:VV2559.
PATRICi20171960. VBIVibVul40472_2563.

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiSTIGERV.
OrthoDBiEOG6CCH44.

Enzyme and pathway databases

BioCyciVVUL196600:GJ9W-2618-MONOMER.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR010043. UTase/UR.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YJ016.

Entry informationi

Entry nameiGLND_VIBVY
AccessioniPrimary (citable) accession number: Q7MIF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: December 15, 2003
Last modified: June 24, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.