Q7MHZ4 (SYY2_VIBVY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase 2
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase 2
Short name=TyrRS 2

Gene names

Name:	tyrS2
Ordered Locus Names:	VV2723

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02007
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02007
Subunit structure	Homodimer By similarity. HAMAP MF_02007
Subcellular location	Cytoplasm By similarity HAMAP MF_02007.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Tyrosine--tRNA ligase 2 HAMAP MF_02007		PRO_0000236779
Regions
	Domain	334 – 394	61	S4 RNA-binding
	Motif	42 – 51	10	"HIGH" region HAMAP MF_02007
	Motif	226 – 230	5	"KMSKS" region HAMAP MF_02007
Sites
	Binding site	229	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MHZ4 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: EA950AA7B3541907	FASTA	395	43,834
	10 20 30 40 50 60 MASIEAALAE IKRGVEELIP EEELIAKLKE GRPLRIKLGA DPTAPDIHLG HTVILNKLRA 70 80 90 100 110 120 FQDLGHDVTF LIGDFTGMVG DPTGKNSTRP PLTREDVLRN AETYKQQVFK ILDPAKTKIQ 130 140 150 160 170 180 FNSEWLSELG AEGMIRLAAN QTVARMLERD DFKKRYAGGQ PIAIHEFMYP LLQGYDSVAM 190 200 210 220 230 240 ETDVELGGTD QKFNLLMGRE LQKANGQKPQ VVLMMPLLVG LDGEKKMSKS ANNYIGVSEA 250 260 270 280 290 300 PSEMFGKIMS ISDDLMWSYY ELLSFRPLEE LAQFKADVAA GKNPRDIKVL LAKEIIARFH 310 320 330 340 350 360 SEADADAAEQ EFVNRFAKNQ IPDEMPEFTF AAGTPMANLL KEAELCSSTS EAMRMVKQGA 370 380 390 AKMDGEKVED AKAEPAVGTY VFQVGKRKFA RITIA

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YJ016.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000037 Genomic DNA. Translation: BAC95487.1.
RefSeq	NP_935516.1. NC_005139.1.
3D structure databases
ProteinModelPortal	Q7MHZ4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7MHZ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2625533.
GenomeReviews	Gene locus VV2723 in contig BA000037_GR.
KEGG	vvy:VV2723.
NMPDR	fig|196600.1.peg.2791.
PATRIC	20172274. VBIVibVul40472_2718.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0162.
HOGENOM	HBG288125.
OMA	IPTYFEL.
PhylomeDB	Q7MHZ4.
ProtClustDB	PRK05912.
Enzyme and pathway databases
BioCyc	VVUL196600:VV2723-MONOMER.
Family and domain databases
HAMAP	MF_02007. Tyr_tRNA_synth_type2. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. IPR024108. Tyr-tRNA-synth_bac_2. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY2_VIBVY

Accession

Primary (citable) accession number: Q7MHZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program