Reviewed, UniProtKB/Swiss-Prot Q7MHL0 (E4PD_VIBVY)

Names and origin

Protein names

Recommended name:
    D-erythrose-4-phosphate dehydrogenase
      Short name=E4PDH
    EC=1.2.1.72

Gene names

Name:	epd
Ordered Locus Names:	VV2859

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent conversion of D-erythrose 4-phosphate to 4-phosphoerythronate By similarity.
Catalytic activity	D-erythrose 4-phosphate + NAD+ + H2O = 4-phosphoerythronate + NADH. HAMAP MF_01640
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 1/5. HAMAP MF_01640
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. Epd subfamily.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal phosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro erythrose-4-phosphate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	D-erythrose-4-phosphate dehydrogenase HAMAP MF_01640		PRO_0000293177
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	163 – 165	3	Substrate binding Potential
	Region	222 – 223	2	Substrate binding Potential
Sites
	Active site	164	1	Nucleophile By similarity
	Binding site	209	1	Substrate Potential
	Binding site	245	1	Substrate Potential
	Binding site	327	1	NAD By similarity
	Site	191	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MHL0-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 645B7FFE1F82C837	FASTA	346	38,189
	10 20 30 40 50 60 MLKVAINGFG RIGRNVLRAV YESGKHQQIK VVAVNELAQP EAMAHLLQYD TSHGRFGKRI 70 80 90 100 110 120 SHDQEHLYVH HDACPQGKGE FDSIRILHLS EINLLPWRDL EVDLVLDCTG VFGCQADGLE 130 140 150 160 170 180 HIKAGAKKVL FSHPGASDLD NTIIYGVNHE TLKAEHNVVS NGSCTTNCIV PIIKVLDEAF 190 200 210 220 230 240 GIESGTITTI HSSMNDQQVI DAYHSDLRRT RAASQSIIPV DTKLHKGIER IFPKFSNKFE 250 260 270 280 290 300 AISVRVPTVN VTAMDLSVTI NTNVKVNDVN QTIVNASQCT LRGIVDYTEA PLVSIDFNHD 310 320 330 340 PHSAIVDGSQ TRVSNGHLVK MLVWCDNEWG FANRMLDTAL AMQAAK

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000037 Genomic DNA. Translation: BAC95623.1.
RefSeq	NP_935652.1.
3D structure databases
HSSP	HSSP built from PDB template 1GD1 based on UniProtKB P00362.
ModBase	Search...
Genome annotation databases
GeneID	2625677.
GenomeReviews	Gene locus VV2859 in contig BA000037_GR.
KEGG	vvy:VV2859.
NMPDR	fig|196600.1.peg.2927.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7MHL0.
OMA	Q7MHL0. AMDLSVT.
Enzyme and pathway databases
BioCyc	VVUL196600:VV2859-MON.
Family and domain databases
HAMAP	MF_01640. [Tree]
InterPro	IPR006422. E4P_DH_bac. IPR000173. GlycerAld_3-P_DH. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01532. E4PD_g-proteo. 1 hit.
PROSITE	PS00071. GAPDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E4PD_VIBVY

Accession

Primary (citable) accession number: Q7MHL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)