Q7MHK1 (GSHB_VIBVY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione synthetase
EC=6.3.2.3

Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase

Gene names

Name:	gshB
Ordered Locus Names:	VV2868

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio vulnificus

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutathione synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Glutathione synthetase HAMAP-Rule MF_00162		PRO_0000197494
Regions
	Domain	125 – 311	187	ATP-grasp
	Nucleotide binding	151 – 208	58	ATP By similarity
Sites
	Metal binding	282	1	Magnesium or manganese By similarity
	Metal binding	284	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MHK1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 10B0BAEA9F189AF8	FASTA	318	35,429
	10 20 30 40 50 60 MMIKLGIVMD PISSINIKKD SSFAMMLEAQ RRGWEIHYME MNDLHLDQGV AIADTKVVQL 70 80 90 100 110 120 KEDPNGWYEF TSEQTIELSE LDAVLMRKDP PFDTEYIYAT YILERAEEQG TLIVNKPQSL 130 140 150 160 170 180 RDCNEKLFTA WFPELTPTTI VTRKAEKIKA FRQEHGDIIL KPLDGMGGAS IFRVKENDPN 190 200 210 220 230 240 VSVIIETLTN HGQNYAMAQT FVPDISNGDK RILVVDGEPM PYCLARIPAK GETRGNLAAG 250 260 270 280 290 300 GSGEPRPLSE TDLKIANAVA PTLKEKGLIF VGLDVIGDKL TEINVTSPTC IREIEAAFDI 310 SITGKLMDAI ERRLQAQA

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YJ016.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000037 Genomic DNA. Translation: BAC95632.1.
RefSeq	NP_935661.1. NC_005139.1.
3D structure databases
ProteinModelPortal	Q7MHK1.
SMR	Q7MHK1. Positions 2-315.
ModBase	Search...
Protein-protein interaction databases
STRING	196600.VV2868.
Proteomic databases
PRIDE	Q7MHK1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC95632; BAC95632; BAC95632.
GeneID	2625686.
KEGG	vvy:VV2868.
PATRIC	20172564. VBIVibVul40472_2855.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0189.
HOGENOM	HOG000265022.
KO	K01920.
OMA	WMRKDPP.
ProtClustDB	PRK05246.
Enzyme and pathway databases
UniPathway	UPA00142; UER00210.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit.
HAMAP	MF_00162. GSH-S.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP-bd. IPR004215. GSHS_N. IPR016185. PreATP-grasp_dom. [Graphical view]
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01380. glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_VIBVY

Accession

Primary (citable) accession number: Q7MHK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program